MicroRNA-192 suppresses cell proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes by downregulating caveolin 1

2017 ◽  
Vol 432 (1-2) ◽  
pp. 123-130 ◽  
Author(s):  
Supin Li ◽  
Zhenmu Jin ◽  
Xiaobing Lu
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Human Rheumatoid Arthritis ◽  
Caveolin 1 ◽  
Fibroblast Like Synoviocytes

MicroRNA-16-5p Promoted Fibroblast-Like Synoviocyte Proliferation and Suppressed Apoptosis via Targeting Suppressor of Cytokine Signaling 6 (SOCS6) in Human Rheumatoid Arthritis

2021 ◽  
Vol 11 (9) ◽  
pp. 1744-1751
Author(s):  
Deqian Meng ◽  
Wenyou Pan ◽  
Ju Li
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Luciferase Reporter Assay ◽  
Luciferase Reporter ◽  
Cytokine Signaling ◽  
Human Rheumatoid Arthritis ◽  
Reporter Assay ◽  
Functional Roles ◽  
Proliferation And Apoptosis ◽  
Fibroblast Like Synoviocytes

Accumulating evidence have indicated that MicroRNAs (miRNAs) are key regulators in human rheumatoid arthritis (RA). The aim of this study was to explore the functional roles of miR-16-5p in proliferation, inflammation, and apoptosis of fibroblast-like synoviocytes (FLS). The expression of miR-16-5p and SOCS6 in FLA was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation and apoptosis were measured by CCK-8 assay and flow cytometry, respectively. Luciferase reporter assay was used to verify the direct target of miR-16-5p. Western blot analysis was performed to analysis the levels of SOCS6, Bcl-2, Bax and cleaved caspase 3. miR-16-5p expression was significantly upregulated while SOCS6 level was decreased in RA-FLS compared with normal FLS. In addition, luciferase reporter assay confirmed that SOCS6 was the target of miR-16-5p. Silencing of miR-16-5p inhibited cell proliferation, releases of TNF-α, IL-1β, IL-6 and IL-8, and induced the apoptosis. The effects of miR-16-5p silencing on RA-FLS were reversed by downregulation of SOCS6. In summary, knockdown of miR-16-5p could suppress cell proliferation and accelerate the apoptosis of RA-FLS through targeting SOCS6, which may provide a potential therapeutic target for patients with RA.

scholarly journals LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

EBioMedicine ◽  
2019 ◽  
Vol 50 ◽  
pp. 408-420 ◽  
Author(s):  
Xuan Bi ◽  
Xing Hua Guo ◽  
Bi Yao Mo ◽  
Man Li Wang ◽  
Xi Qing Luo ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Migration And Invasion ◽  
Fibroblast Like Synoviocytes

scholarly journals miR-483-3p promotes cell proliferation and suppresses apoptosis in rheumatoid arthritis fibroblast-like synoviocytes by targeting IGF-1

2020 ◽  
Vol 130 ◽  
pp. 110519
Author(s):  
YueJiao Wang ◽  
LinXin Hou ◽  
XiaoWei Yuan ◽  
NeiLi Xu ◽  
Shuai Zhao ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Fibroblast Like Synoviocytes

scholarly journals Modulation of platelet-derived microparticles to adhesion and motility of human rheumatoid arthritis fibroblast-like synoviocytes

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181003 ◽  
Author(s):  
Wenwen Wang ◽  
Jiahuan Liu ◽  
Binzhou Yang ◽  
Zhongshuang Ma ◽  
Guiping Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Rheumatoid Arthritis ◽  
Fibroblast Like Synoviocytes
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