Cisplatin-induced human peripheral blood mononuclear cells’ oxidative stress and nephrotoxicity in head and neck cancer patients: the influence of hydrogen peroxide

2017 ◽  
Vol 440 (1-2) ◽  
pp. 139-145 ◽  
Author(s):  
Júlia C. F. Quintanilha ◽  
Marília B. Visacri ◽  
Vanessa M. Sousa ◽  
Larissa B. Bastos ◽  
Camila O. Vaz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Head And Neck Cancer ◽  
Cancer Patients ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Solaleh Emamgholipour ◽  
Arash Hossein-Nezhad ◽  
Mohammad Ansari
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Fold Increase ◽  
Gene Expressions ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Purpose.We aimed to investigate the possible effects of melatonin on gene expressions and activities of MnSOD and catalase under conditions of oxidative stress induced by hydrogen peroxide (H2O2) in peripheral blood mononuclear cells (PBMCs).Materials and Methods.PBMCs were isolated from healthy subjects and treated as follows: (1) control (only with 0.1% DMSO for 12 h); (2) melatonin (1 mM) for 12 h; (3) H2O2(250 μM) for 2 h; (4) H2O2(250 μM) for 2 h following 10 h pretreatment with melatonin (1 mM). The gene expression was evaluated by real-time PCR. MnSOD and catalase activities in PBMCs were determined by colorimetric assays.Results.Pretreatment of PBMCs with melatonin significantly augmented expression and activity of MnSOD which were diminished by H2O2. Melatonin treatment of PBMCs caused a significant upregulation of catalase by almost 2-fold in comparison with untreated cells. However, activity and expression of catalase increased by 1.5-fold in PBMCs under H2O2-induced oxidative stress compared with untreated cell. Moreover, pretreatment of PBMCs with melatonin resulted in a significant 1.8-fold increase in catalase expression compared to PBMCs treated only with H2O2.Conclusion.It seems that melatonin could prevent from undesirable impacts of H2O2-induced oxidative stress on MnSOD downregulation. Moreover, melatonin could promote inductive effect of H2O2on catalase mRNA expression.

scholarly journals Malvidin prevents lipopolysaccharide‐induced oxidative stress and inflammation in human peripheral blood mononuclear cells

IUBMB Life ◽  
2020 ◽  
Vol 72 (7) ◽  
pp. 1504-1514 ◽  
Author(s):  
Ali R. Bastin ◽  
Asie Sadeghi ◽  
Moslem Abolhassani ◽  
Amir H. Doustimotlagh ◽  
Abbas Mohammadi
Keyword(s):  
Oxidative Stress ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Methamidophos induces cytotoxicity and oxidative stress in human peripheral blood mononuclear cells

2015 ◽  
Vol 32 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Marco Antonio Ramirez-Vargas ◽  
Gerardo Huerta-Beristain ◽  
Iris Paola Guzman-Guzman ◽  
Luz del Carmen Alarcon-Romero ◽  
Eugenia Flores-Alfaro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
And Oxidative Stress
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