scholarly journals An efficient and cost-effective approach for genic microsatellite marker-based large-scale trait association mapping: identification of candidate genes for seed weight in chickpea

2014 ◽  
Vol 34 (1) ◽  
pp. 241-265 ◽  
Author(s):  
Alice Kujur ◽  
Deepak Bajaj ◽  
Maneesha S. Saxena ◽  
Shailesh Tripathi ◽  
Hari D. Upadhyaya ◽  
...  
1997 ◽  
Vol 36 (8-9) ◽  
pp. 307-311 ◽  
Author(s):  
R. Y. G. Andoh ◽  
C. Declerck

Rapid urbanisation and its consequent increase in impermeable surface areas and changes in land use has generally resulted in problems of flooding and heavy pollution of urban streams and other receiving waters. This has often been coupled with ground water depletion and a threat to water resources. The first part of this paper presents an alternative drainage philosophy and strategy which mimics nature's way by slowing down (attenuating) the movement of urban runoff. This approach results in cost-effective, affordable and sustainable drainage schemes. The alternative strategy can be described as one of prevention rather than cure by effecting controls closer to source rather than the traditional approach which results in the transfer of problems downstream, resulting in its cumulation and the need for large scale, centralised control. The second part describes a research project which has been launched in order to quantify the cost and operational benefits of source control and distributed storage. Details of the methodology of the modelling and simulation processes which are being followed to achieve this target are presented.


2019 ◽  
Vol 21 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Kaiyu Xie ◽  
Ali Reza Kamali

Molten salt electrolytic reduction of Fe2O3 in the presence of water is proposed as a sustainable and cost-effective approach for large-scale production of iron.


Environmental science and management is often the most discussed subject nowadays all over the world. In a number of countries, presently plenty of harms are associated with the effluent by industrial due to growing industrialization; this issue should be considered at large scale. Textile sector is one of the leading areas, which uses a high amount of chemicals and creating environmental pollution. Textile wet processing sector uses a lot of chemicals, surfactants and synthetic dyes, hence produce a large amount of wastewater having a high concentration of chemicals. This research is an effort to investigate the amount of residue remained in liquor after bleaching and reuses this liquor by adding a few chemicals according to the requirement. Afterward, the comparison was made between the bleached sample with fresh liquor and bleached sample with reused liquor. It was observed that bleaching with reused liquor shows good results though these results are insignificantly less than fresh bleaching liquor. On the other hand, fortunately, the color yield of dyed fabric bleached with reused liquor is higher than fresh liquor.


Author(s):  
D. T. Ingersoll ◽  
Z. J. Houghton ◽  
R. Bromm ◽  
C. Desportes

Nuclear energy plants are attractive energy source for large scale water desalination since the thermal energy produced in a nuclear reactor can provide both electricity and steam to desalt water without the production of greenhouse gases. A particularly attractive option is to couple a desalination plant with the new generation of nuclear plant designs: small modular reactors (SMR). This allows regions with smaller electrical grids and limited infrastructure to add new electrical and water capacity in more appropriate increments and allows countries to consider siting plants at a broader range of distributed locations. The NuScale SMR plant design is especially well suited for the co-generation of electricity and desalted water. The enhanced safety, improved affordability, and deployment flexibilities of the NuScale design provide a cost-effective approach to expanding global desalination capacity. Parametric studies have been performed to evaluate technical options for coupling a NuScale plant to a variety of different desalination technologies. An economic comparison of these options was performed for each of the different desalination technologies coupled to an appropriately sized NuScale plant capable of providing sufficient carbon-free electricity and clean water to support a city of 300,000 people.


2017 ◽  
Author(s):  
Jian Li ◽  
Aierken Yiming ◽  
Ping Wang

AbstractHereditary retinal disease (HRD) is a series of Mendelian diseases affecting the retina in the eye. The genetic basis of HRD is very complicated, with more than 100 disease-causing genes being identified. Though NGS has allowed rapid and large-scale mutation screening of Mendelian disease, the cost of NGS still prevents its universal application all over the world, for an accurate molecular diagnosis. Here, by clinical guidance from patient phenotypes, we performed targeted molecular diagnosis by direct Sanger sequencing of the most likely candidate gene in two families diagnosed with HRD. Then we identified two novel protein-truncating variants in the gene CRB1. Our results demonstrated the notion that molecular diagnosis and clinical diagnosis can be mutually supplemented and clinically guided direct sequencing is a cost-effective approach for molecular diagnosis and subsequent genetic counseling.


2019 ◽  
Author(s):  
Gianpiero Marconi ◽  
Stefano Capomaccio ◽  
Cinzia Comino ◽  
Alberto Acquadro ◽  
Ezio Portis ◽  
...  

AbstractMethods for investigating DNA methylation nowadays either require a reference genome and high coverage, or investigate only CG methylation. Moreover, no large-scale analysis can be performed for N6-methyladenosine (6mA). Here we describe the methylation content sensitive enzyme double-digest restriction-site-associated DNA (ddRAD) technique (MCSeEd), a reduced-representation, reference-free, cost-effective approach for characterizing whole genome methylation patterns across different methylation contexts (e.g., CG, CHG, CHH, 6mA). MCSeEd can also detect genetic variations among hundreds of samples. MCSeEd is based on parallel restrictions carried out by combinations of methylation insensitive and sensitive endonucleases, followed by next-generation sequencing. Moreover, we present a robust bioinformatic pipeline (available at https://bitbucket.org/capemaster/mcseed/src/master/) for differential methylation analysis combined with single nucleotide polymorphism calling without or with a reference genome.


2013 ◽  
Vol 115 (12) ◽  
pp. 1-35
Author(s):  
Stuart S. Yeh

Background In principle, value-added modeling (VAM) might be justified if it can be shown to be a more reliable indicator of teacher quality than existing indicators for existing low-stakes decisions that are already being made, such as the award of small merit bonuses. However, a growing number of researchers now advocate the use of VAM to identify and replace large numbers of low-performing teachers. There is a need to evaluate these proposals because the active termination of large numbers of teachers based on VAM requires a much higher standard of reliability and validity. Furthermore, these proposals must be evaluated to determine if they are cost-effective compared to alternative proposals for raising student achievement. While VAM might be justified as a replacement for existing indicators (for existing decisions regarding merit compensation), it might not meet the higher standard of reliability and validity required for large-scale teacher termination, and it may not be the most cost-effective approach for raising student achievement. If society devotes its resources to approaches that are not cost-effective, the increase in achievement per dollar of resources expended will remain low, inhibiting reduction of the achievement gap. Objective This article reviews literature regarding the reliability and validity of VAM, then focuses on an evaluation of a proposal by Chetty, Friedman, and Rockoff to use VAM to identify and replace the lowest-performing 5% of teachers with average teachers. Chetty et al. estimate that implementation of this proposal would increase the achievement and lifetime earnings of students. The results appear likely to accelerate the adoption of VAM by school districts nationwide. The objective of the current article is to evaluate the Chetty et al. proposal and the strategy of raising student achievement by using VAM to identify and replace low-performing teachers. Method This article analyzes the assumptions of the Chetty et al. study and the assumptions of similar VAM-based proposals to raise student achievement. This analysis establishes a basis for evaluating the Chetty et al. proposal and, in general, a basis for evaluating all VAM-based policies to raise achievement. Conclusion VAM is not reliable or valid, and VAM-based polices are not cost-effective for the purpose of raising student achievement and increasing earnings by terminating large numbers of low-performing teachers.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gianpiero Marconi ◽  
Stefano Capomaccio ◽  
Cinzia Comino ◽  
Alberto Acquadro ◽  
Ezio Portis ◽  
...  

Abstract Methods for investigating DNA methylation nowadays either require a reference genome and high coverage, or investigate only CG methylation. Moreover, no large-scale analysis can be performed for N6-methyladenosine (6 mA) at an affordable price. Here we describe the methylation content sensitive enzyme double-digest restriction-site-associated DNA (ddRAD) technique (MCSeEd), a reduced-representation, reference-free, cost-effective approach for characterizing whole genome methylation patterns across different methylation contexts (e.g., CG, CHG, CHH, 6 mA). MCSeEd can also detect genetic variations among hundreds of samples. MCSeEd is based on parallel restrictions carried out by combinations of methylation insensitive and sensitive endonucleases, followed by next-generation sequencing. Moreover, we present a robust bioinformatic pipeline (available at https://bitbucket.org/capemaster/mcseed/src/master/) for differential methylation analysis combined with single nucleotide polymorphism calling without or with a reference genome.


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