Mapping of two loci conferring resistance to wheat stem rust pathogen races TTKSK (Ug99) and TRTTF in the elite hard red spring wheat line SD4279

Humphreys, D. G., Townley-Smith, T. F., Lukow, O. M., McCallum, B. D., Fetch, T. G., Gilbert, J. A., Menzies, J. G., Tkachuk, V., Brown, P. D. and Fox, S. L. 2014. Peace hard red spring wheat. Can. J. Plant Sci. 94: 1297–1302. Peace is a hard red spring wheat (Triticum aestivum L.) adapted to the shorter-season wheat-growing regions of the Canadian prairies. Peace was evaluated in the Parkland Wheat Cooperative Test in 1999, 2000 and 2001. Peace exhibited grain yield similar to the check cultivars over 2 yr (1999–2000; Neepawa and Roblin) and over 3 yr (1999–2001; AC Barrie and AC Splendor) Peace matured a day earlier than AC Barrie but was 2 d later than AC Splendor over 3 yr of testing (1999–2001) and was a day later than Neepawa and Roblin over 2 yr of testing (1999–2000). Peace had test weight similar to the check cultivars. Peace was moderately resistant to leaf rust and loose smut and resistant to stem rust including the highly virulent Ug99 race of stem rust and common bunt. Peace was moderately susceptible to Fusarium head blight. Peace meets the end-use quality specifications of the Canada Western Red Spring wheat class.

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Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Nature Communications ◽

10.1038/s41467-019-12927-7 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 20

Author(s):

Feng Li ◽

Narayana M. Upadhyaya ◽

Jana Sperschneider ◽

Oadi Matny ◽

Hoa Nguyen-Phuc ◽

...

Keyword(s):

Stem Rust ◽

Wheat Stem Rust ◽

Haploid Nucleus ◽

Somatic Hybridisation ◽

Global Food Security ◽

Proximity Analysis ◽

Rust Pathogen ◽

Fungal Populations ◽

Nuclear Exchange ◽

Global Food

Abstract Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.

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Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen

Theoretical and Applied Genetics ◽

10.1007/s00122-014-2442-4 ◽

2014 ◽

Vol 128 (3) ◽

pp. 431-443 ◽

Cited By ~ 15

Author(s):

Guotai Yu ◽

Qijun Zhang ◽

Timothy L. Friesen ◽

Matthew N. Rouse ◽

Yue Jin ◽

...

Keyword(s):

Stem Rust ◽

Aegilops Tauschii ◽

Wheat Stem Rust ◽

Tauschii Accession ◽

Rust Pathogen

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AC Abbey hard red spring wheat

Canadian Journal of Plant Science ◽

10.4141/p99-089 ◽

2000 ◽

Vol 80 (1) ◽

pp. 123-127 ◽

Cited By ~ 16

Author(s):

R. M. DePauw ◽

J. M. Clarke ◽

R. E. Knox ◽

M. R. Fernandez ◽

T. N. McCaig ◽

...

Keyword(s):

Triticum Aestivum ◽

Spring Wheat ◽

Stem Rust ◽

Wheat Yield ◽

Triticum Aestivum L ◽

Common Bunt ◽

Canadian Prairies ◽

Wheat Stem Sawfly ◽

Hard Red Spring Wheat ◽

Moderate Resistance

AC Abbey, hard red spring wheat (Triticum aestivum L.), is adapted to the Canadian prairies. It is significantly shorter than any of the check cultivars and has solid stems. AC Abbey expressed higher grain yield, earlier maturity, and heavier kernels than AC Eatonia, the solidstem check cultivar. It is resistant to the wheat stem sawfly (Cephus cinctus Nort.) and to prevalent races of common bunt and has moderate resistance to leaf rust and stem rust. AC Abbey is eligible for grades of Canada Western Red Spring wheat. Key words: Triticum aestivum L., red spring wheat, yield, wheat stem sawfly, plant height, maturity

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A Genome-Wide Association Study of Field and Seedling Response to Individual Stem Rust Pathogen Races Reveals Combinations of Race-Specific Genes in North American Spring Wheat

Frontiers in Plant Science ◽

10.3389/fpls.2018.00052 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 11

Author(s):

Erena A. Edae ◽

Michael O. Pumphrey ◽

Matthew N. Rouse

Keyword(s):

Association Study ◽

Spring Wheat ◽

North American ◽

Stem Rust ◽

Genome Wide Association Study ◽

Genome Wide Association ◽

Genome Wide ◽

A Genome ◽

Rust Pathogen

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Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

10.1101/692640 ◽

2019 ◽

Cited By ~ 1

Author(s):

Feng Li ◽

Narayana M. Upadhyaya ◽

Jana Sperschneider ◽

Oadi Matny ◽

Hoa Nguyen-Phuc ◽

...

Keyword(s):

Stem Rust ◽

Puccinia Graminis ◽

Wheat Stem Rust ◽

Haploid Nucleus ◽

Somatic Hybridisation ◽

Global Food Security ◽

Proximity Analysis ◽

Rust Pathogen ◽

Nuclear Exchange ◽

Global Food

AbstractParasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here we show that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis revealed that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or reassortment. Generation of genetic variation by nuclear exchange may favour the evolution of dikaryotism by providing an advantage over diploidy.

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The effects of selected factors on measured ergot alkaloid content in Claviceps purpurea-infected hexaploid and durum wheat

World Mycotoxin Journal ◽

10.3920/wmj2015.2019 ◽

2016 ◽

Vol 9 (4) ◽

pp. 555-564 ◽

Cited By ~ 3

Author(s):

S.A. Tittlemier ◽

D. Drul ◽

M. Roscoe ◽

J.G. Menzies

Keyword(s):

Durum Wheat ◽

Host Plant ◽

Spring Wheat ◽

Ergot Alkaloid ◽

Ergot Alkaloids ◽

Alkaloid Content ◽

Claviceps Purpurea ◽

Wheat Line ◽

Hard Red Spring Wheat ◽

Plant Line

Four wheat genotypes, including the ergot-susceptible durum ‘AC Avonlea’ and hard red spring wheat ‘AC Cadillac’, as well as the resistant durum wheat line 9260B-173A and the hard red spring wheat line ‘Kenya Farmer’ wereinoculated with different Claviceps purpurea isolates. Honeydew and sclerotia were collected and analysed for 10 ergot alkaloids. Total concentrations of the 10 ergot alkaloids ranged from 16 µg/kg in honeydew to 1,798 mg/kg insclerotia. Ergonovine and ergosine were the predominant alkaloids in honeydew obtained from plants inoculated with various isolates, whereas ergocristine and ergocryptine were the main alkaloids observed in sclerotia. Bothhost plant and C. purpurea isolate were significant factors affecting total ergot alkaloid concentrations in sclerotia. Irrespective of host plant line, all mean total ergot alkaloid concentrations were higher in sclerotia produced from the EI-2 isolate (695-1,010 mg/kg), as compared to EI-4 (255-594 mg/kg). The mass of total ergot alkaloids was alsopositively correlated with the mass of individual sclerotia produced from these two C. purpurea isolates, with the slope of the regression higher for the EI-2 isolate. The total ergot alkaloid concentrations in sclerotia from various plants inoculated with the same C. purpurea isolate differed; however, the resistance of host plant line did notappear to be consistent with ergot alkaloid content in sclerotia. Concentrations of total ergot alkaloids were highestand lowest in sclerotia from the two lines that are both classified as ‘resistant’, suggesting that the mechanism ofresistance for these lines is not restriction on the production of ergot alkaloids in sclerotia.

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