Cold stress in broiler: global gene expression analyses suggest a major role of CYP genes in cold responses

2011 ◽  
Vol 39 (1) ◽  
pp. 425-429 ◽  
Author(s):  
Xingyong Chen ◽  
Runshen Jiang ◽  
Zhaoyu Geng
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Global Gene Expression ◽  
Gene Expression Analyses ◽  
Cold Responses

scholarly journals Multiple Known Mechanisms and a Possible Role of an Enhanced Immune System in Bt-Resistance in a Field Population of the Bollworm, Helicoverpa zea: Differences in Gene Expression with RNAseq

2020 ◽  
Vol 21 (18) ◽  
pp. 6528
Author(s):  
Roger D. Lawrie ◽  
Robert D. Mitchell III ◽  
Jean Marcel Deguenon ◽  
Loganathan Ponnusamy ◽  
Dominic Reisig ◽  
...  
Keyword(s):  
Gene Expression ◽  
Serine Proteases ◽  
Helicoverpa Zea ◽  
Insect Pests ◽  
Global Gene Expression ◽  
Field Resistance ◽  
Bt Resistance ◽  
Imd Pathway ◽  
Immune Pathway

Several different agricultural insect pests have developed field resistance to Bt (Bacillus thuringiensis) proteins (ex. Cry1Ac, Cry1F, etc.) expressed in crops, including corn and cotton. In the bollworm, Helicoverpa zea, resistance levels are increasing; recent reports in 2019 show up to 1000-fold levels of resistance to Cry1Ac, a major insecticidal protein in Bt-crops. A common method to analyze global differences in gene expression is RNA-seq. This technique was used to measure differences in global gene expression between a Bt-susceptible and Bt-resistant strain of the bollworm, where the differences in susceptibility to Cry1Ac insecticidal proteins were 100-fold. We found expected gene expression differences based on our current understanding of the Bt mode of action, including increased expression of proteases (trypsins and serine proteases) and reduced expression of Bt-interacting receptors (aminopeptidases and cadherins) in resistant bollworms. We also found additional expression differences for transcripts that were not previously investigated, i.e., transcripts from three immune pathways-Jak/STAT, Toll, and IMD. Immune pathway receptors (ex. PGRPs) and the IMD pathway demonstrated the highest differences in expression. Our analysis suggested that multiple mechanisms are involved in the development of Bt-resistance, including potentially unrecognized pathways.

scholarly journals Mechanistic elements and critical factors of cellular reprogramming revealed by stepwise global gene expression analyses

2014 ◽  
Vol 12 (3) ◽  
pp. 730-741 ◽  
Author(s):  
Sung-Jin Park ◽  
Hock Chuan Yeo ◽  
Nam-Young Kang ◽  
Hanjo Kim ◽  
Joyce Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Global Gene Expression ◽  
Cellular Reprogramming ◽  
Critical Factors ◽  
Gene Expression Analyses

The role of the Shigella flexneri yihE gene in LPS synthesis and virulence

Microbiology ◽  
2004 ◽  
Vol 150 (4) ◽  
pp. 1079-1084 ◽  
Author(s):  
Bryn Edwards-Jones ◽  
Paul R. Langford ◽  
J. Simon Kroll ◽  
Jun Yu
Keyword(s):  
Gene Expression ◽  
Global Change ◽  
Unknown Function ◽  
Guinea Pigs ◽  
Antimicrobial Agents ◽  
Shigella Flexneri ◽  
Global Gene Expression ◽  
Wild Type ◽  
In Trans

Previously, the authors have shown that inactivation of Shigella flexneri yihE, a gene of unknown function upstream of dsbA, which encodes a periplasmic disulphide catalyst, results in a global change of gene expression. Among the severely down-regulated genes are galETKM, suggesting that the yihE mutant, Sh54, may inefficiently produce the UDP-glucose and UDP-galactose required for LPS synthesis. This paper demonstrates that LPS synthesis in Sh54 is impaired. As a result, Sh54 is unable to polymerize host cell actin, due to aberrant localization of IcsA, or to cause keratoconjunctivitis in guinea pigs. Furthermore, Sh54 is more sensitive to some antimicrobial agents, and exhibits epithelial cytotoxicity characteristic of neither wild-type nor dsbA mutants. Supplying galETK in trans restores LPS synthesis and corrects all the defects. Hence, it is clear that the Shigella yihE gene is important not only in regulating global gene expression, as shown previously, but also in virulence through LPS synthesis via regulating the expression of the galETK operon.

scholarly journals Global gene expression analyses of hematopoietic stem cell-like cell lines with inducible Lhx2 expression

BMC Genomics ◽  
2006 ◽  
Vol 7 (1) ◽  
Author(s):  
Karin Richter ◽  
Valtteri Wirta ◽  
Lina Dahl ◽  
Sara Bruce ◽  
Joakim Lundeberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Lines ◽  
Global Gene Expression ◽  
Hematopoietic Stem ◽  
Gene Expression Analyses
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