Attenuation of cellular toxicity by calpain inhibitor induced by bacterial endotoxin: a mechanistic study using muscle precursor cells as a model system

2015 ◽  
Vol 42 (8) ◽  
pp. 1281-1288 ◽  
Author(s):  
Ke Shang ◽  
Junfeng Zhang ◽  
Touseef Amna ◽  
Jieun Yang ◽  
Xiangchao Cheng ◽  
...  
Keyword(s):  
Model System ◽  
Precursor Cells ◽  
Bacterial Endotoxin ◽  
Mechanistic Study ◽  
Calpain Inhibitor ◽  
Cellular Toxicity ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

506: Restoration of Innervated Urinary Sphincter Function Using Muscle Precursor Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 135-135
Author(s):  
Rene Yiou ◽  
James J. Yoo ◽  
Anthony Atala
Keyword(s):  
Precursor Cells ◽  
Sphincter Function ◽  
Muscle Precursor ◽  
Urinary Sphincter ◽  
Muscle Precursor Cells

scholarly journals Fibroblast growth factor 2-stimulated proliferation is lower in muscle precursor cells from old rats

2009 ◽  
Vol 94 (6) ◽  
pp. 739-748 ◽  
Author(s):  
Seth S. Jump ◽  
Tom E. Childs ◽  
Kevin A. Zwetsloot ◽  
Frank W. Booth ◽  
Simon J. Lees
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Precursor Cells ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Muscle Precursor ◽  
Old Rats ◽  
Muscle Precursor Cells

scholarly journals Regulation and Function of SF/HGF during Migration of Limb Muscle Precursor Cells in Chicken

1996 ◽  
Vol 180 (2) ◽  
pp. 566-578 ◽  
Author(s):  
Silke Heymann ◽  
Maria Koudrova ◽  
H.-H. Arnold ◽  
Markus Köster ◽  
Thomas Braun
Keyword(s):  
Precursor Cells ◽  
Limb Muscle ◽  
Muscle Precursor ◽  
And Function ◽  
Muscle Precursor Cells

A role for inflammatory mediators in the modulation of the neurotrophin receptor p75NTR on human muscle precursor cells

2012 ◽  
Vol 243 (1-2) ◽  
pp. 100-102 ◽  
Author(s):  
Emanuela Colombo ◽  
Stefania Romaggi ◽  
Marina Mora ◽  
Lucia Morandi ◽  
Cinthia Farina
Keyword(s):  
Inflammatory Mediators ◽  
Precursor Cells ◽  
Human Muscle ◽  
Neurotrophin Receptor ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

An evaluation of cell separation techniques in a model mixed cell population

1992 ◽  
Vol 102 (4) ◽  
pp. 789-798
Author(s):  
S.J. Murphy ◽  
D.J. Watt ◽  
G.E. Jones
Keyword(s):  
Cell Population ◽  
Cell Separation ◽  
Separation Efficiency ◽  
Neuronal Cell ◽  
Quantitative Measure ◽  
Precursor Cells ◽  
Muscle Fibres ◽  
Myogenic Cells ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

Muscle precursor cells may act not only as a means of inserting normal genes into diseased muscle fibres, in order to correct or alleviate a genetically inherited myopathy, but recent demonstrations have shown they may prove an invaluable tool for the expression of, and systemic dissemination of, non-muscle gene products. If muscle precursor cells are proved to act as such widespread vectors in terms of gene therapy, then it is imperative that methods are properly elucidated to produce large populations of pure viable myogenic cells for such purposes. In the past, many methods of cell separation have been investigated but carry with them the problems of either a lack of myogenic purity of the population or poor percentage recovery of the original cell population. In the present work we have investigated two methods for segregating myogenic from non-myogenic cells and have critically reviewed the efficiency of separation of the two techniques used. To obtain a quantitative measure of separation efficiency, segregation was carried out on a 1:1 mixture of murine C2 myogenic and murine 3T3 fibroblastic cells. To distinguish between C2 and 3T3 cells, the latter were prelabelled with the fluorescent strain carboxyfluorescein diacetate succinimyl ester (CFSE). Once incorporated into the cell, CFSE remains there, thus preventing transfer of the label to C2 cells. Both methods of separation used depend on the affinity of myogenic cells for the monoclonal antibody Mab H28, which specifically binds to the mouse neuronal cell adhesion molecule N-CAM, but differ in that one method, “panning”, completes segregation by adherence of N-CAM positive cells to a dish precoated with secondary IgG antibody whereas in the other separation proceeds by the use of commercially available IgG-coated magnetic beads. Results indicate magnetic bead separation to be more efficient than panning if the beads are precoated with 0.1% gelatin.

The role of Lbx1 in migration of muscle precursor cells

Development ◽  
2000 ◽  
Vol 127 (2) ◽  
pp. 437-445 ◽  
Author(s):  
H. Brohmann ◽  
K. Jagla ◽  
C. Birchmeier
Keyword(s):  
Homeobox Gene ◽  
Branchial Arch ◽  
Precursor Cells ◽  
Muscle Precursor ◽  
Hindlimb Muscles ◽  
Expression Of Genes ◽  
Extensor Muscles ◽  
Septum Transversum ◽  
Muscle Precursor Cells

The homeobox gene Lbx1 is expressed in migrating hypaxial muscle precursor cells during development. These precursors delaminate from the lateral edge of the dermomyotome and form distinct streams that migrate over large distances, using characteristic paths. The targets of migration are limbs, septum transversum and the floor of the first branchial arch where the cells form skeletal muscle of limbs and shoulders, diaphragm and hypoglossal cord, respectively. We used gene targeting to analyse the function of Lbx1 in the mouse. Myogenic precursor cells delaminate from the dermomyotome in Lbx1 mutants, but migrate in an aberrant manner. Most critically affected are migrating cells that move to the limbs. Precursor cells that reach the dorsal limb field are absent. In the ventral limb, precursors are present but distributed in an abnormal manner. As a consequence, at birth some muscles in the forelimbs are completely lacking (extensor muscles) or reduced in size (flexor muscles). Hindlimb muscles are affected strongly, and distal limb muscles are more affected than proximal ones. Other migrating precursor cells heading towards the floor of the first branchial arch move along the appropriate path in Lbx1 mutants. However, these cells migrate less efficiently and reduced numbers of precursors reach their distal target. At birth, the internal lingual muscle is therefore reduced in size. We suggest that Lbx1 controls the expression of genes that are essential for the recognition or interpretation of cues that guide migrating muscle precursors and maintain their migratory potential.

rolling pebbles(rols) is required inDrosophilamuscle precursors for recruitment of myoblasts for fusion

Development ◽  
2001 ◽  
Vol 128 (24) ◽  
pp. 5061-5073 ◽  
Author(s):  
Annette Rau ◽  
Detlev Buttgereit ◽  
Anne Holz ◽  
Richard Fetter ◽  
Stephen K. Doberstein ◽  
...  
Keyword(s):  
Amino Acids ◽  
Transcription Initiation ◽  
Muscle Development ◽  
Precursor Cells ◽  
Malpighian Tubules ◽  
Muscle Precursor ◽  
Terminal Amino ◽  
Founder Cells ◽  
Fusion Competent Myoblasts ◽  
Muscle Precursor Cells

Mutations in the rolling pebbles (rols) gene result in severe defects in myoblast fusion. Muscle precursor cells are correctly determined, but myogenesis does not progress significantly beyond this point because recognition and/or cell adhesion between muscle precursor cells and fusion-competent myoblasts is disturbed. Molecular analysis of the rols genomic region reveals two variant transcripts of rols due to different transcription initiation sites, rols6 and rols7. rols6 mRNA is detectable mainly in the endoderm during differentiation as well as in malpighian tubules and in the epidermis. By contrast, rols7 expression is restricted to the mesoderm and later to progenitor descendants during somatic and pharyngeal muscle development. Transcription starts at the extended germ band stage when progenitor/founder cells are determined and persists until stage 13. The proteins encoded by the rols gene are 1670 (Rols6) and 1900 (Rols7) amino acids in length. Both forms contain an N-terminal RING-finger motif, nine ankyrin repeats and a TPR repeat eventually overlaid by a coiled-coil domain. The longer protein, Rols7, is characterized by 309 unique N-terminal amino acids, while Rols6 is distinguishable by 79 N-terminal amino acids. Expression of rols7 in muscle founder cells indicates a function of Rols7 in these cells. Transplantation assays of rols mutant mesodermal cells into wild-type embryos show that Rols is required in muscle precursor cells and is essential to recruit fusion-competent myoblasts for myotube formation.

Microcarrier culture of muscle precursor cells for clinical application

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S90-S91
Author(s):  
W. Tra ◽  
J. Prange ◽  
S. Burer ◽  
D. Mohr ◽  
R. Das ◽  
...  
Keyword(s):  
Clinical Application ◽  
Precursor Cells ◽  
Microcarrier Culture ◽  
Muscle Precursor ◽  
Muscle Precursor Cells
Sign in / Sign up

Export Citation Format

Share Document