Mitochondrial DNA markers reveal genetic connectivity among populations of Osteoglossiform fish Chitala chitala

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

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Concordance of microsatellite and mitochondrial DNA markers in detecting genetic population structure in the boring giant clamTridacna croceaacross the Indo-Malay Archipelago

Marine Ecology ◽

10.1111/maec.12389 ◽

2016 ◽

Vol 38 (1) ◽

pp. e12389 ◽

Cited By ~ 7

Author(s):

Min Hui ◽

Agus Nuryanto ◽

Marc Kochzius

Keyword(s):

Mitochondrial Dna ◽

Population Structure ◽

Dna Markers ◽

Genetic Population Structure ◽

Genetic Population ◽

Malay Archipelago

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Molecular Methods for the Detection of Natural Hybrids in Sturgeon Populations

Transylvanian Review of Systematical and Ecological Research ◽

10.1515/trser-2015-0003 ◽

2015 ◽

Vol 16 (1) ◽

pp. 65-72 ◽

Cited By ~ 1

Author(s):

Alexandru Burcea ◽

Iulia Elena Florescu ◽

Andreea Dudu ◽

Sergiu Emil Georgescu ◽

Marieta Costache

Keyword(s):

Mitochondrial Dna ◽

Dna Markers ◽

Molecular Methods ◽

Hybrid Species ◽

Habitat Area ◽

Natural Hybrids ◽

Sturgeon Species ◽

Morphological Criteria ◽

Hybrid Sturgeon

Abstract Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented by microsatellites and mitochondrial DNA. This identification data and methodology is important for use on sturgeon farms due to the need to correctly identify species of sturgeons. Using the proposed methodologies, it is possible to avoid identification errors that might appear when using only morphological criteria to idenfy sturgeons

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A phylogenetic framework for the bumblebee species of the subgenus Bombus sensu stricto based on mitochondrial DNA markers, with a short description of the neglected taxon B. minshanicola Bischoff, 1936 n. status. (Hymenoptera: Apidae: Bombus).

Beiträge zur Entomologie = Contributions to Entomology ◽

10.21248/contrib.entomol.60.2.471-487 ◽

2010 ◽

Vol 60 (2) ◽

pp. 471-487

Author(s):

Andreas Bertsch

Keyword(s):

Mitochondrial Dna ◽

Cytochrome Oxidase ◽

Dna Markers ◽

Sensu Stricto ◽

Short Description ◽

Bumblebee Species ◽

Neue Art ◽

Phylogenetic Framework ◽

Bombus Affinis ◽

N Status

Königinnen von 12 Taxa der Untergattung Bombus sensu stricto (Bombus affinis, B. albocinctus, B. cryptarum, B. lucorum, B. magnus, B. moderatus, B. occidentalis, B. patagiatus, B. sporadicus, B. terrestris, B. terricola and B. tunicatus) wurden im Frühjahr gefangen, um künstliche Kolonien zu züchten. Zusätzlich wurden Männchen von B. franklini und B. lucorum/China gefangen. Mitochondriale Cytochrome Oxidase Untereinheit I (COI) von 53 Proben wurde sequenziert (Teilsequenzen der Länge 1257 bp). GenBank Sequenzen der Ostasiatischen Arten B. hypocrita, B. ignitus und B. lucorum/China wurden einbezogen. Der Unterschied zwischen den Arten beträgt 30 – 92 Basen-Substitutionen und die Tamura-Nei genetische Distanz 0.030-0.103, während der Unterschied innerhalb der Arten nur 1 – 3 Basensubstitution beträgt und die Tamura-Nei genetische Distanz nur 0.001-0.003. Dreizehn Taxa mit Artrang sowie eine neue Art B. minshanicola Bischoff 1936 (= B. terrestris ssp. minshanicola = B. lucorum/China) wurden nachgewiesen. In der Topologie des phylogenetischen Stammbaums gibt es 4 Cluster: ein Artenpaar B. sporadicus – B. ignitus und ein Artenpaar B. terricola – B. occidentalis, ein Cluster für B. magnus, B. patagiatus and B. cryptarum mit den Subspecies B. cryptarum albocinctus und B. cryptarum moderatus sowie ein Cluster für B. hypocrita, B. lucorum, B. minshanicola, B. franklini and B. affinis. Die Arten B. terrestris mit den Subspecies B. terrestris canariensis und B. terrestris sassaricus sowie B. tunicatus sind einzeln abgetrennt. Da sich im Alignment der COI Sequenzen keine Lücken finden, können die einzelnen Nucleotid-Positionen als homolog betrachtet werden. Jede Art besitzt eine Reihe einzigartiger (‘privater’) Positionen, die als diagnostische Merkmale benutzt werden können um die Art zu definieren und zu identifizieren.

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