Species-specific mitochondrial DNA markers for identification of non-invasive samples from sympatric carnivores in the Iberian Peninsula

2007 ◽  
Vol 9 (3) ◽  
pp. 681-690 ◽  
Author(s):  
Carlos A. Fernandes ◽  
Catarina Ginja ◽  
Iris Pereira ◽  
Rogério Tenreiro ◽  
Michael W. Bruford ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Iberian Peninsula ◽  
Dna Markers ◽  
Non Invasive ◽  
Sympatric Carnivores ◽  
Species Specific

scholarly journals First Insight into Groundwater Copepods of the Polish Lowland

Water ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2086
Author(s):  
Maciej Karpowicz ◽  
Sabina Smolska ◽  
Magdalena Świsłocka ◽  
Joanna Moroz
Keyword(s):  
Mitochondrial Dna ◽  
Ribosomal Rna ◽  
Dna Markers ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Morphological Description ◽  
Single Plate ◽  
North Eastern ◽  
Insight Into

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

scholarly journals A Comparative Study of 5S rDNA Non-Transcribed Spacers in Elaeagnaceae Species

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 4
Author(s):  
Oleg S. Alexandrov ◽  
Olga V. Razumova ◽  
Gennady I. Karlov
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Markers ◽  
Dna Markers ◽  
5S Rdna ◽  
Chain Reaction ◽  
Closely Related Species ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Species Specific ◽  
Shepherdia Canadensis

5S rDNA is organized as a cluster of tandemly repeated monomers that consist of the conservative 120 bp coding part and non-transcribed spacers (NTSs) with different lengths and sequences among different species. The polymorphism in the 5S rDNA NTSs of closely related species is interesting for phylogenetic and evolutional investigations, as well as for the development of molecular markers. In this study, the 5S rDNA NTSs were amplified with universal 5S1/5S2 primers in some species of the Elaeagnaceae Adans. family. The polymerase chain reaction (PCR) products of five Elaeagnus species had similar lengths near 310 bp and were different from Shepherdia canadensis (L.) Nutt. and Sh. argentea (Pusch.) Nutt. samples (260 bp and 215 bp, respectively). The PCR products were cloned and sequenced. An analysis of the sequences revealed that intraspecific levels of NTS identity are high (approximately 95–96%) and similar in the Elaeagnus L. species. In Sh. argentea, this level was slightly lower due to the differences in the poly-T region. Moreover, the intergeneric and intervarietal NTS identity levels were studied and compared. Significant differences between species (except E. multiflora Thunb. and E. umbellata Thunb.) and genera were found. Herein, a range of the NTS features is discussed. This study is another step in the investigation of the molecular evolution of Elaeagnaceae and may be useful for the development of species-specific DNA markers in this family.

scholarly journals A Review of Murine Cytomegalovirus as a Model for Human Cytomegalovirus Disease—Do Mice Lie?

2020 ◽  
Vol 22 (1) ◽  
pp. 214
Author(s):  
Michelle A. Fisher ◽  
Megan L. Lloyd
Keyword(s):  
Human Cytomegalovirus ◽  
Murine Cytomegalovirus ◽  
Clinical Samples ◽  
Mouse Strains ◽  
Cytomegalovirus Disease ◽  
Wild Mice ◽  
Congenital Cytomegalovirus ◽  
Non Invasive ◽  
Species Specific ◽  
Disseminated Disease

Since murine cytomegalovirus (MCMV) was first described in 1954, it has been used to model human cytomegalovirus (HCMV) diseases. MCMV is a natural pathogen of mice that is present in wild mice populations and has been associated with diseases such as myocarditis. The species-specific nature of HCMV restricts most research to cell culture-based studies or to the investigation of non-invasive clinical samples, which may not be ideal for the study of disseminated disease. Initial MCMV research used a salivary gland-propagated virus administered via different routes of inoculation into a variety of mouse strains. This revealed that the genetic background of the laboratory mice affected the severity of disease and altered the extent of subsequent pathology. The advent of genetically modified mice and viruses has allowed new aspects of disease to be modeled and the opportunistic nature of HCMV infection to be confirmed. This review describes the different ways that MCMV has been used to model HCMV diseases and explores the continuing difficulty faced by researchers attempting to model HCMV congenital cytomegalovirus disease using the mouse model.

Plasma-derived cell-free mitochondrial DNA: A novel non-invasive methodology to identify mitochondrial DNA haplogroups in humans

2018 ◽  
Vol 125 (4) ◽  
pp. 332-337 ◽  
Author(s):  
Christopher Newell ◽  
Stacey Hume ◽  
Steven C. Greenway ◽  
Lynn Podemski ◽  
Jane Shearer ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Non Invasive

Quantitative Detection of Species-Specific DNA in Feedstuffs and Fish Meals

2005 ◽  
Vol 68 (6) ◽  
pp. 1217-1221 ◽  
Author(s):  
PAVEL KRCMAR ◽  
EVA RENCOVA
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Dna Sequences ◽  
Real Time Pcr ◽  
Single Species ◽  
Absolute Quantification ◽  
Quantitative Detection ◽  
Vertebrate Species ◽  
Target Species ◽  
Species Specific

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species–derived material in concentrate feed mixtures and fish meals.

scholarly journals Molecular Methods for the Detection of Natural Hybrids in Sturgeon Populations

2015 ◽  
Vol 16 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Alexandru Burcea ◽  
Iulia Elena Florescu ◽  
Andreea Dudu ◽  
Sergiu Emil Georgescu ◽  
Marieta Costache
Keyword(s):  
Mitochondrial Dna ◽  
Dna Markers ◽  
Molecular Methods ◽  
Hybrid Species ◽  
Habitat Area ◽  
Natural Hybrids ◽  
Sturgeon Species ◽  
Morphological Criteria ◽  
Hybrid Sturgeon

Abstract Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented by microsatellites and mitochondrial DNA. This identification data and methodology is important for use on sturgeon farms due to the need to correctly identify species of sturgeons. Using the proposed methodologies, it is possible to avoid identification errors that might appear when using only morphological criteria to idenfy sturgeons

scholarly journals A phylogenetic framework for the bumblebee species of the subgenus Bombus sensu stricto based on mitochondrial DNA markers, with a short description of the neglected taxon B. minshanicola Bischoff, 1936 n. status. (Hymenoptera: Apidae: Bombus).

2010 ◽  
Vol 60 (2) ◽  
pp. 471-487
Author(s):  
Andreas Bertsch
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Dna Markers ◽  
Sensu Stricto ◽  
Short Description ◽  
Bumblebee Species ◽  
Neue Art ◽  
Phylogenetic Framework ◽  
Bombus Affinis ◽  
N Status

Königinnen von 12 Taxa der Untergattung Bombus sensu stricto (Bombus affinis, B. albocinctus, B. cryptarum, B. lucorum, B. magnus, B. moderatus, B. occidentalis, B. patagiatus, B. sporadicus, B. terrestris, B. terricola and B. tunicatus) wurden im Frühjahr gefangen, um künstliche Kolonien zu züchten. Zusätzlich wurden Männchen von B. franklini und B. lucorum/China gefangen. Mitochondriale Cytochrome Oxidase Untereinheit I (COI) von 53 Proben wurde sequenziert (Teilsequenzen der Länge 1257 bp). GenBank Sequenzen der Ostasiatischen Arten B. hypocrita, B. ignitus und B. lucorum/China wurden einbezogen. Der Unterschied zwischen den Arten beträgt 30 – 92 Basen-Substitutionen und die Tamura-Nei genetische Distanz 0.030-0.103, während der Unterschied innerhalb der Arten nur 1 – 3 Basensubstitution beträgt und die Tamura-Nei genetische Distanz nur 0.001-0.003. Dreizehn Taxa mit Artrang sowie eine neue Art B. minshanicola Bischoff 1936 (= B. terrestris ssp. minshanicola = B. lucorum/China) wurden nachgewiesen. In der Topologie des phylogenetischen Stammbaums gibt es 4 Cluster: ein Artenpaar B. sporadicus – B. ignitus und ein Artenpaar B. terricola – B. occidentalis, ein Cluster für B. magnus, B. patagiatus and B. cryptarum mit den Subspecies B. cryptarum albocinctus und B. cryptarum moderatus sowie ein Cluster für B. hypocrita, B. lucorum, B. minshanicola, B. franklini and B. affinis. Die Arten B. terrestris mit den Subspecies B. terrestris canariensis und B. terrestris sassaricus sowie B. tunicatus sind einzeln abgetrennt. Da sich im Alignment der COI Sequenzen keine Lücken finden, können die einzelnen Nucleotid-Positionen als homolog betrachtet werden. Jede Art besitzt eine Reihe einzigartiger (‘privater’) Positionen, die als diagnostische Merkmale benutzt werden können um die Art zu definieren und zu identifizieren.

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