scholarly journals The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants

2021 ◽  
Author(s):  
Alireza Abbasi ◽  
Meysam Malekpour ◽  
Sajjad Sobhanverdi
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Drought Stress Tolerance ◽  
Expansin Gene

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

scholarly journals The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants

2021 ◽  
Author(s):  
Alireza Abbasi ◽  
Meysam Malekpour ◽  
Sajjad Sobhanverdi
Keyword(s):  
Drought Stress ◽  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Dry Weight ◽  
Conventional Polymerase Chain Reaction ◽  
Specific Gene ◽  
Severe Drought ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Reverse Transcription Pcr

Abstract Expansins are cell wall proteins that, due to changes in pH, causing the expansion of the cell walls. In this study, a previously gene construct designed based on a root-specific gene, AtEXPA18, was utilized to assess its potential roles on different morphological, physiological, and cellular levels of generated transgenic tobacco plants in response to moderate and severe drought stress. AtEXPA18 gene was successfully transferred to the tobacco plants through an agrobacterium-mediate transformation system. Upon obtaining the second generation, tobacco transgenic plants were confirmed by conventional polymerase chain reaction (PCR) technique alongside reverse transcription PCR (RT-PCR) using specific primers. Under drought stress, the transgenic lines showed remarkable growth and significantly improved based on morphological traits such as height and stem diameter, leaf area, leaf number, root dry weight, and Abscisic acid (ABA) levels of leaves compared control plants. As a result, the Cytokinin content of transgenic plants has increased under severe stress levels. Notably, the area's expansion for abaxial epidermal cells under the microscope confirmed in transgene cells compared with the -transgene cells. These results, altogether, could support the AtEXPA18 gene implication in cell expansion and improving tolerance capacity of transgenic crops under drought stress.

Root growth of transgenic tobacco plants with constitutive expression of the expansin gene PnEXPA3 under the action of stress factors.

Biomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 545-551
Author(s):  
Z.A. Berezhneva ◽  
B.R. Kuluev
Keyword(s):  
Heavy Metals ◽  
Transgenic Tobacco ◽  
Constitutive Expression ◽  
Stress Factors ◽  
Cultivated Plants ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants ◽  
Expansin Gene ◽  
Agricultural Plants

Plant α-expansin genes are of great interest for genetic engineering, since they can be used to improve the growth of the roots of agricultural plants. Previously, we obtained transgenic tobacco plants Nicotiana tabacum overexpressing the PnEXPA3 expansin gene from black poplar Populus nigra. In this work, the growth of roots of transgenic plants was studied under normal conditions and under the influence of stress factors such as hypothermia, salinity and heavy metals. Constitutive expression of the PnEXPA3 gene improved the growth of roots of transgenic tobacco plants as compared to the wild type under normal conditions and under hypothermia (+10°C), salinity (50 and 100 mM NaCl) and the action of heavy metals (200 and 400 μM acetate cadmium). The totality of the obtained data suggests that the constitutive expression of the PnEXPA3 gene can be used to improve the growth of roots of cultivated plants both under normal conditions and the action of stress factors.

Enhanced tolerance to drought stress in transgenic tobacco plants overexpressing VTE1 for increased tocopherol production from Arabidopsis thaliana

2008 ◽  
Vol 30 (7) ◽  
pp. 1275-1280 ◽  
Author(s):  
Xiaoli Liu ◽  
Xuejun Hua ◽  
Juan Guo ◽  
Dongmei Qi ◽  
Lijuan Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants
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