Development of a GFP-Expressing Aspergillus flavus Strain to Study Fungal Invasion, Colonization, and Resistance in Cottonseed

2008 ◽  
Vol 165 (2) ◽  
pp. 89-97 ◽  
Author(s):  
Kanniah Rajasekaran ◽  
Jeffrey W. Cary ◽  
Peter J. Cotty ◽  
Thomas E. Cleveland
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Invasion

scholarly journals Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of Aspergillus flavus to study fungal invasion, colonization, and competition in maize kernels

2014 ◽  
Vol 5 ◽  
Author(s):  
Zuzana Hruska ◽  
Kanniah Rajasekaran ◽  
Haibo Yao ◽  
Russell Kincaid ◽  
Dawn Darlington ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Invasion ◽  
Maize Kernels

The 'omics' tools: genomics, proteomics, metabolomics and their potential for solving the aflatoxin contamination problem

2008 ◽  
Vol 1 (1) ◽  
pp. 3-12 ◽  
Author(s):  
D. Bhatnagar ◽  
K. Rajasekaran ◽  
G. Payne ◽  
R. Brown ◽  
J. Yu ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin Contamination ◽  
Control Measures ◽  
Clear Understanding ◽  
Fungal Invasion ◽  
Toxigenic Fungi ◽  
Additional Information ◽  
Time Control ◽  
Crop Resistance ◽  
Food And Feed

Aflatoxins are highly carcinogenic secondary metabolites produced primarily by the fungi Aspergillus flavus and Aspergillus parasiticus. Aflatoxin contamination of food and feed is an age old problem of particular concern over the last four decades. Now, for the first time control measures for this problem appear within reach. For practical and sustainable control of aflatoxin contamination to be realised, however, additional information is needed rather rapidly, particularly for understanding the specific molecular factors (both in the plant and the fungus) involved during host plant-fungus interaction. The information derived from the use of novel tools such as genomics, proteomics and metabolomics provides us with the best and the quickest opportunity to achieve a clear understanding of the survival of toxigenic fungi in the field, the ability of the fungus to invade crops, and the process of toxin contamination under various environmental conditions. Significant progress has been made recently in understanding the genomic makeup of the most significant aflatoxin producing field fungus, namely Aspergillus flavus. Progress also has been made in the study of host crop resistance to fungal invasion through the use of proteomics. The information available on production of aflatoxin and other metabolites by Aspergillus flavus is reasonably extensive, although the application of metabolomics as a tool in this study is relatively new. In this review there is a discussion of the use of genomics, proteomics and metabolomics in deriving the requisite information for developing effective strategies to interrupt the machinery in the fungus for production of these toxins, as well as to assist in the development of host-resistance against fungal invasion and aflatoxin contamination of crops.

Systemic aspergillosis caused by an aflatoxin-producing strain of Aspergillus flavus

1998 ◽  
Vol 36 (2) ◽  
pp. 107-112 ◽  
Author(s):  
T. MORI ◽  
M. MATSUMURA ◽  
K. YAMADA ◽  
S. IRIE ◽  
K. OSHIMI ◽  
...  
Keyword(s):  
Aspergillus Flavus

Identificação de Espécies de Aspergillus e Potencial Toxígeno de Aspergillus flavus Isolados de Rações Comerciais

2014 ◽  
Vol 16 (2) ◽  
pp. 131-136
Author(s):  
V.C. Alves ◽  
F.C. Cardoso Filho ◽  
M.M.G. Pereira ◽  
A.P.R. Costa ◽  
M.C.S. Muratori
Keyword(s):  
Aspergillus Flavus

Synthesis, photosensitization and antimicrobial activity evaluation of some novel Merocyanine dyes

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Flavus ◽  
Elemental Analysis ◽  
Cyanine Dyes ◽  
Fungal Strains ◽  
Activity Evaluation ◽  
Visible Spectra ◽  
Merocyanine Dyes

Novel acyclic and cyclic merocyanine dyes derived from the nucleu of furo [(3,2-d) pyrazole; ( d 2 , 3 )imidazole]were prepared. The electronic visible absorptionspectra of all the synthesized new cyanine dyes were examined in 95% ethanolsolution to evaluate their photosensitization properties. Antibacterial andantifungal activities for some selected dyes were tested against various bacterialand fungal strains (Escherichia coli, Staphylococcus aureus, Aspergillus flavus andCandida albicans) to evaluate their antimicrobial activity. Structural identificationwas carried out via elemental analysis, visible spectra, IR and 1H NMRspectroscopic data.

Chemical Composition and Active Properties Evaluation of Wild Oregano (Origanum Vulgare) and Ginger (Zingiber Officinale-Roscoe) Essential Oils

2018 ◽  
Vol 69 (8) ◽  
pp. 1927-1933 ◽  
Author(s):  
Mariana Deleanu ◽  
Elisabeta E. Popa ◽  
Mona E. Popa
Keyword(s):  
Essential Oil ◽  
Growth Inhibition ◽  
Gallic Acid ◽  
Aspergillus Flavus ◽  
Zingiber Officinale ◽  
Penicillium Expansum ◽  
Origanum Vulgare ◽  
Zingiber Officinale Roscoe ◽  
Oregano Oil ◽  
Gallic Acid Equivalent

The compounds in Ginger (Zingiber officinale-Roscoe) essential oil provenience China and wild oregano (Origanum vulgare) essential oil of Romanian origin were identified by GC/MS and their antioxidant and antifungal properties were evaluated. Wild oregano oil was characterized by high content of oxygenated monoterpenes hydrocarbons (84.05%) of which carvacrol was the most abundant (73.85%) followed by b-linalool (3.46%) and thymol (2.29%). Ginger oil had a higher content of sesquiterpene hydrocarbons including zingiberene (31.47%), b-sesquiphellandrene (13.76%), a-curcumene (10.41%), a-farnesene (8.31%) and b-bisabolene (7.55%) but a lower content of oxygenated monoterpenes (7.97%). The high content of oxygenated monoterpens of wild oregano oil is in accordance with total content of polyphenols determined by the Folin�Ciocalteu method (6.71�0.73 mg of gallic acid equivalent per g oil). Ginger oil had only 1.34�0.22 mg gallic acid equivalent per g oil. Wild oregano oils exhibited appreciable in vitro antioxidant activity as assessed by 2, 2`-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and 2,2�-azino-bis (3 ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). The sample concentration required to scavenge 50% of the DPPH free radicals was 0.76�0.13 mg/mL for wild oregano oil compared to 20.22�2.12 mg/mL for ginger oil. Also, wild oregano oils showed significant inhibitory activity against selected pathogenic fungi (Fusarium oxysporum, Aspergillus flavus and Penicillium expansum). 1�L of oregano oil is sufficient for almost 75% growth inhibition of Aspergillus flavus compared to ginger oil which shows antifungal activity at 240�L for 78% growth inhibition. It can be concluded that wild oregano oil could be used as food preservative in some food products in which Fusarium oxysporum, Aspergillus flavus and Penicillium expansum could grow and have potential to produce health hazards mycotoxines.

EGF-Mediated Suppression of Cell Extrusion During Mucosal Damage Attenuates Opportunistic Fungal Invasion

2020 ◽  
Author(s):  
Sebastian Wurster ◽  
Oscar E. Ruiz ◽  
Krystin M. Samms ◽  
Alexander M. Tatara ◽  
Nathaniel D. Albert ◽  
...  
Keyword(s):  
Mucosal Damage ◽  
Fungal Invasion ◽  
Cell Extrusion
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