PbGP43 Genotyping Using Paraffin-Embedded Biopsies of Human Paracoccidioidomycosis Reveals a Genetically Distinct Lineage in the Paracoccidioides brasiliensis Complex

2021 ◽  
Author(s):  
Giannina Ricci ◽  
Emeline Boni Campanini ◽  
Angela Satie Nishikaku ◽  
Rosana Puccia ◽  
Mariângela Marques ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Distinct Lineage

scholarly journals PbGP43 Genotyping Using Paraffin-embedded Biopsies of Human Paracoccidioidomycosis Reveals a Genetically Distinct Lineage in the Paracoccidioides Brasiliensis Complex

2021 ◽  
Author(s):  
Giannina Ricci ◽  
Emeline Boni Campanini ◽  
Angela Satie Nishikaku ◽  
Rosana Puccia ◽  
Mariangela Ester Alencar Marques ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Paracoccidioides Brasiliensis ◽  
Haplotype Network ◽  
Histopathological Diagnosis ◽  
Gene Products ◽  
High Genetic Diversity ◽  
Tissue Samples ◽  
Ffpe Samples ◽  
Distinct Lineage ◽  
Phylogenetic Lineages

Abstract Paracoccidioidomycosis (PCM) is a systemic mycosis caused by a group of cryptic species embedded in the Paracoccidioides brasiliensis complex and Paracoccidioides lutzii. Four species were recently inferred to belong to the P. brasiliensis complex, but the high genetic diversity found in both human and environmental samples have suggested that the number of lineages may be higher.This study aimed to assess the 43-kilodalton glycoprotein genotypes (PbGP43) in paraffin-embedded samples from PCM patients to infer the phylogenetic lineages of the P. brasiliensis complex responsible for causing the infection.Formalin-fixed, paraffin-embedded (FFPE) tissue samples from patients with histopathological diagnosis of PCM were analyzed. DNAs were extracted and amplified for a region of the second exon of the PbGP43 gene. Products were sequenced and aligned with other PbGP43 sequences available. A haplotype network and the phylogenetic relationships among sequences were inferred. Amino acid substitutions were investigated regarding the potential to modify physicochemical properties in the proteins.Six phylogenetic lineages were identified as belonging to the P. brasiliensis complex. Two lineages did not group with any of the four recognized species of the complex, and, interestingly, one of them comprised only FFPE samples. A coinfection involving two lineages was found. Five parsimony-informative sites were identified and three of them showed radical non-synonymous substitutions with the potential to promote changes in the protein.This study expands the knowledge regarding the genetic diversity existing in the P. brasiliensis complex and shows the potential of FFPE samples in species identification and in detecting coinfections.

Identification, N-terminal region sequencing and similarity analysis of differentially expressed proteins in Paracoccidioides brasiliensis

1999 ◽  
Vol 37 (2) ◽  
pp. 115-121 ◽  
Author(s):  
A. F. Cunha ◽  
M. V. Sousa ◽  
S. P. Silva ◽  
R. S. A. JesuIno ◽  
C. M. A. Soares ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Terminal Region ◽  
Differentially Expressed ◽  
Similarity Analysis ◽  
Differentially Expressed Proteins

Recognition of Fungal Components by the Host Immune System

2020 ◽  
Vol 21 (3) ◽  
pp. 245-264 ◽  
Author(s):  
Laura C. García-Carnero ◽  
José A. Martínez-Álvarez ◽  
Luis M. Salazar-García ◽  
Nancy E. Lozoya-Pérez ◽  
Sandra E. González-Hernández ◽  
...  
Keyword(s):  
Immune System ◽  
Histoplasma Capsulatum ◽  
Paracoccidioides Brasiliensis ◽  
Sporothrix Schenckii ◽  
Clinical Importance ◽  
Pathogenic Fungi ◽  
Diagnostic Tools ◽  
Future Research ◽  
Host Immune System ◽  
Fungal Antigens

: By being the first point of contact of the fungus with the host, the cell wall plays an important role in the pathogenesis, having many molecules that participate as antigens that are recognized by immune cells, and also that help the fungus to establish infection. The main molecules reported to trigger an immune response are chitin, glucans, oligosaccharides, proteins, melanin, phospholipids, and others, being present in the principal pathogenic fungi with clinical importance worldwide, such as Histoplasma capsulatum, Paracoccidioides brasiliensis, Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Blastomyces dermatitidis, and Sporothrix schenckii. Knowledge and understanding of how the immune system recognizes and responds to fungal antigens are relevant for the future research and development of new diagnostic tools and treatments for the control of mycosis caused by these fungi.

scholarly journals Metabolic Peculiarities of Paracoccidioides brasiliensis Dimorphism as Demonstrated by iTRAQ Labeling Proteomics

2019 ◽  
Vol 10 ◽  
Author(s):  
Danielle Silva Araújo ◽  
Maristela Pereira ◽  
Igor Godinho Portis ◽  
Agenor de Castro Moreira dos Santos Junior ◽  
Wagner Fontes ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Itraq Labeling

Brazilian Red Propolis shows antifungal and immunomodulatory activities against Paracoccidioides brasiliensis

2021 ◽  
pp. 114181
Author(s):  
Lauana Aparecida Santos ◽  
Pedro Luiz Rosalen ◽  
Nayara Andrade Dias ◽  
Julianne Caravita Grisolia ◽  
Bruno José Nascimento Gomes ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis

scholarly journals Protective Response in Experimental Paracoccidioidomycosis Elicited by Extracellular Vesicles Containing Antigens of Paracoccidioides brasiliensis

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1813
Author(s):  
Ludmila Matos Baltazar ◽  
Gabriela Fior Ribeiro ◽  
Gustavo J. Freitas ◽  
Celso Martins Queiroz-Junior ◽  
Caio Tavares Fagundes ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Treatment Effectiveness ◽  
Ex Vivo ◽  
Paracoccidioides Brasiliensis ◽  
Systemic Disease ◽  
Host Immune System ◽  
Antigen Delivery ◽  
Activated T Lymphocytes ◽  
Cell Mobilization

Paracoccidioidomycosis (PCM) is a systemic disease caused by Paracoccidioides spp. PCM is endemic in Latin America and most cases are registered in Brazil. This mycosis affects mainly the lungs, but can also spread to other tissues and organs, including the liver. Several approaches have been investigated to improve treatment effectiveness and protection against the disease. Extracellular vesicles (EVs) are good antigen delivery vehicles. The present work aims to investigate the use of EVs derived from Paracoccidioides brasiliensis as an immunization tool in a murine model of PCM. For this, male C57BL/6 were immunized with two doses of EVs plus adjuvant and then infected with P. brasiliensis. EV immunization induced IgM and IgG in vivo and cytokine production by splenocytes ex vivo. Further, immunization with EVs had a positive effect on mice infected with P. brasiliensis, as it induced activated T lymphocytes and NKT cell mobilization to the infected lungs, improved production of proinflammatory cytokines and the histopathological profile, and reduced fungal burden. Therefore, the present study shows a new role for P. brasiliensis EVs in the presence of adjuvant as modulators of the host immune system, suggesting their utility as immunizing agents.

scholarly journals Ecology of Paracoccidioides brasiliensis, P. lutzii and related species: infection in armadillos, soil occurrence and mycological aspects

2018 ◽  
Author(s):  
Marluce Francisca Hrycyk ◽  
Hans Garcia Garces ◽  
Sandra de Moraes Gimenes Bosco ◽  
Sílvio Luis de Oliveira ◽  
Sílvio Alencar Marques ◽  
...  
Keyword(s):  
Related Species ◽  
Paracoccidioides Brasiliensis

Paracoccidioidomycosis due to Paracoccidioides brasiliensis S1 associated with acquired immunodeficiency syndrome: A case report

2020 ◽  
Author(s):  
Paulo Mendes Peçanha ◽  
Isabela Cruz Bahiense ◽  
Wdson Luis Lima Kruschewsky ◽  
Cláudia Biasutti ◽  
Carlos Urbano Gonçalves Ferreira Júnior ◽  
...  
Keyword(s):  
Case Report ◽  
Acquired Immunodeficiency Syndrome ◽  
Paracoccidioides Brasiliensis ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

Outbreaks of Vesicular Stomatitis in Brazil caused by a distinct lineage of Alagoas vesiculovirus

2021 ◽  
Author(s):  
Anapolino Macedo de Oliveira ◽  
Mateus Laguardia-Nascimento ◽  
Mariana Lázaro Sales ◽  
Anselmo Rivetti Vasconcelos Júnior ◽  
Marcelo Fernandes Camargos ◽  
...  
Keyword(s):  
Distinct Lineage ◽  
Vesicular Stomatitis
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