Cerium oxide nanoparticles protect primary mouse bone marrow stromal cells from apoptosis induced by oxidative stress

Applications of europium-doped NaYF4 (NaYF4:Eu3+) nanoparticles in biomedical fields will inevitably increase their exposure to humans, therefore, the assessment of toxicities must be taken into consideration.

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Cysteine Dioxygenase Type 1 Inhibits Osteogenesis by Regulating Wnt Signaling in Primary Mouse Bone Marrow Stromal Cells

Scientific Reports ◽

10.1038/srep19296 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 6

Author(s):

Xuefeng Zhao ◽

Peng Deng ◽

Jie Feng ◽

Zheng Wang ◽

Zichao Xiang ◽

...

Keyword(s):

Bone Marrow ◽

Wnt Signaling ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Mouse Bone Marrow ◽

Cysteine Dioxygenase ◽

Primary Mouse

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Mutant U2AF1-induced differential alternative splicing causes an oxidative stress in bone marrow stromal cells

Experimental Biology and Medicine ◽

10.1177/15353702211010130 ◽

2021 ◽

pp. 153537022110101

Author(s):

Zhe Liu ◽

Xuanjia Dong ◽

Zhijie Cao ◽

Shaowei Qiu ◽

Yihui Li ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Bone Marrow ◽

Alternative Splicing ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Hematopoietic Cells ◽

Marrow Stromal Cells ◽

Gene Set Enrichment Analysis ◽

Mouse Bone Marrow

Alternative splicing (AS) is a critical regulatory process of gene expression. In bone marrow microenvironment, AS plays a critical role in mesenchymal stem cells fate determination by forming distinct isoforms of important regulators. As a spliceosome factor, U2AF1 is essential for the catalysis of pre-mRNA splicing, and its mutation can cause differential AS events. In the present study, by forced expression of mutant U2AF1 (U2AF1S34F) in the mouse bone marrow stroma OP9 cells, we determine AS changes in U2AF1S34F transduced OP9 cells and investigate their role in stroma cell biological functions. We find that abundant differential RNA splicing events are induced by U2AF1S34F in OP9 cells. U2AF1S34F causes increased generation of hydrogen peroxide, promotes production of cytokines and chemokines. U2AF1S34F transduced OP9 cells also exhibit dysfunction of mitochondria. RNA-seq data, gene ontology (GO), and gene set enrichment analysis reveal that differentially expressed genes downregulated in response to U2AF1S34F are enriched in peroxisome component and function. U2AF1S34F can also cause release of hydrogen peroxide from OP9 cells. Furthermore, we investigate the influence of U2AF1S34F-induced oxidative stress in stromal cells on hematopoietic cells. When co-culturing mouse bone marrow mononuclear cells with OP9 cells, the U2AF1S34F expressing OP9 cells induce phosphorylation of histone H2AX in hematopoietic cells. Collectively, our results reveal that mutant U2AF1-induced differential AS events cause oxidative stress in bone marrow stromal cells and can further lead to DNA damage and genomic instability in hematopoietic cells.

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Effects of La3+ on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells

Journal of Rare Earths ◽

10.1016/s1002-0721(10)60645-x ◽

2012 ◽

Vol 30 (1) ◽

pp. 90-93 ◽

Cited By ~ 11

Author(s):

Jinchao ZHANG ◽

Jing SUN ◽

Guangqi GU ◽

Xiaohong HAO ◽

Dandan LIU ◽

...

Keyword(s):

Bone Marrow ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Adipogenic Differentiation ◽

Marrow Stromal Cells ◽

Mouse Bone Marrow ◽

Primary Mouse

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Rapamycin inhibits osteoblast proliferation and differentiation in MC3T3-E1 cells and primary mouse bone marrow stromal cells

Journal of Cellular Biochemistry ◽

10.1002/jcb.21411 ◽

2008 ◽

Vol 103 (2) ◽

pp. 434-446 ◽

Cited By ~ 111

Author(s):

Ujjal K. Singha ◽

Yu Jiang ◽

Shibing Yu ◽

Min Luo ◽

Yi Lu ◽

...

Keyword(s):

Bone Marrow ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Mouse Bone Marrow ◽

Osteoblast Proliferation ◽

Primary Mouse ◽

Proliferation And Differentiation

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Influence of Titanium Alloy Scaffolds on Enzymatic Defense against Oxidative Stress and Bone Marrow Cell Differentiation

International Journal of Biomaterials ◽

10.1155/2020/1708214 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Lais Morandini Rodrigues ◽

Elis Andrade Lima Zutin ◽

Elisa Mattias Sartori ◽

Daniela Baccelli Silveira Mendonça ◽

Gustavo Mendonça ◽

...

Keyword(s):

Oxidative Stress ◽

Bone Marrow ◽

Titanium Alloy ◽

Antioxidant Enzymes ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Control Group ◽

Mouse Bone Marrow ◽

Mrna Levels

Studies have been directed towards the production of new titanium alloys, aiming for the replacement of Ti-6 Aluminium-4 Vanadium (TiAlV) alloy in the future. Many mechanisms related to biocompatibility and chemical characteristics have been studied in the field of implantology, but enzymatic defenses against oxidative stress remain underexplored. Bone marrow stromal cells have been explored as source of cells, which have the potential to differentiate into osteoblasts and therefore could be used as cells-based therapy. The objective of this study was to evaluate the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) in porous scaffolds of Ti-6 Aluminium-4 Vanadium (TiAlV), Ti-35 Niobium (TiNb), and Ti-35 Niobium-7 Zirconium-5 Tantalum (TiNbZrTa) on mouse bone marrow stromal cells. Porous titanium alloy scaffolds were prepared by powder metallurgy. After 24 hours, cells plated on the scaffolds were analyzed by scanning electron microscopy (SEM). The antioxidant enzyme activity was measured 72 hours after cell plating. Quantitative real time PCR (qRT-PCR) was performed after 3, 7, and 14 days, and Runx2 (Runt-related transcription factor2) expression was evaluated. The SEM images showed the presence of interconnected pores and growth, adhesion, and cell spreading in the 3 scaffolds. Although differences were noted for SOD and CAT activity for all scaffolds analyzed, no statistical differences were observed (p>0.05). The osteogenic gene Runx2 presented high expression levels for TiNbZrTa at day 7, compared to the control group (TiAlV day 3). At day 14, all scaffolds had more than 2-fold induction for Runx2 mRNA levels, with statistically significant differences compared to the control group. Even though we were not able to confirm statistically significant differences to justify the replacement of TiAlV regarding antioxidant enzymes, TiNbZrTa was able to induce faster bone formation at early time points, making it a good choice for biomedical and tissue bioengineering applications.

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