The p38/CYLD Pathway is Involved in Necroptosis Induced by Oxygen-glucose Deprivation Combined with ZVAD in Primary Cortical Neurons

2017 ◽  
Vol 42 (8) ◽  
pp. 2294-2304 ◽  
Author(s):  
Tao Feng ◽  
WeiWei Chen ◽  
CaiYi Zhang ◽  
Jie Xiang ◽  
HongMei Ding ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Primary Cortical Neurons

scholarly journals SUMO2/3 Conjugation is an Endogenous Neuroprotective Mechanism

2011 ◽  
Vol 31 (11) ◽  
pp. 2152-2159 ◽  
Author(s):  
Anna Lena Datwyler ◽  
Gisela Lättig-Tünnemann ◽  
Wei Yang ◽  
Wulf Paschen ◽  
Sabrina Lin Lin Lee ◽  
...  
Keyword(s):  
Stress Response ◽  
Cerebral Ischemia ◽  
Cortical Neurons ◽  
Neuronal Loss ◽  
Glucose Deprivation ◽  
Transient Cerebral Ischemia ◽  
Oxygen Glucose Deprivation ◽  
Primary Cortical Neurons ◽  
Microrna Delivery

Small ubiquitin-like modifier (SUMO)2/3 but not SUMO1 conjugation is activated after transient cerebral ischemia. To investigate its function, we blocked neuronal SUMO2/3 translation through lentiviral microRNA delivery in primary cortical neurons. Viability was unaffected by SUMO2/3 silencing unless neurons were stressed by transient oxygen–glucose deprivation (OGD). Both 15 and 45 minutes of OGD were tolerated by control microRNA-expressing neurons but damaged >60% of neurons expressing SUMO2/3 microRNA. Damaging OGD (75 minutes) increased neuronal loss to 54% (control microRNA) and to 99% (SUMO2/3 microRNA). This suggests that activation of SUMO2/3 conjugation is an endogenous neuroprotective stress response.

Sign in / Sign up

Export Citation Format

Share Document