Protective Effects of Estrogen via Nanoparticle Delivery to Attenuate Myelin Loss and Neuronal Death after Spinal Cord Injury

2021 ◽  
Author(s):  
Azizul Haque ◽  
Kelsey P. Drasites ◽  
April Cox ◽  
Mollie Capone ◽  
Ali I. Myatich ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Neuronal Death ◽  
Protective Effects ◽  
Nanoparticle Delivery ◽  
Cord Injury

Protective Effects of Zinc on Spinal Cord Injury

2021 ◽  
Author(s):  
Shan Wen ◽  
Yuanlong Li ◽  
Xiaolei Shen ◽  
Zhe Wang ◽  
Kaihua Zhang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Protective Effects ◽  
Cord Injury

scholarly journals Knockdown of long non-coding RNA LEF1-AS1 attenuates apoptosis and inflammatory injury of microglia cells following spinal cord injury

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Sheng-Yu Cui ◽  
Wei Zhang ◽  
Zhi-Ming Cui ◽  
Hong Yi ◽  
Da-Wei Xu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Viability ◽  
Microglial Cells ◽  
Protective Effects ◽  
Loss Of Function ◽  
Sprague Dawley ◽  
Cord Injury ◽  
Apoptosis And Inflammation

Abstract Background Spinal cord injury (SCI) is associated with health burden both at personal and societal levels. Recent assessments on the role of lncRNAs in SCI regulation have matured. Therefore, to comprehensively explore the function of lncRNA LEF1-AS1 in SCI, there is an urgent need to understand its occurrence and development. Methods Using in vitro experiments, we used lipopolysaccharide (LPS) to treat and establish the SCI model primarily on microglial cells. Gain- and loss of function assays of LEF1-AS1 and miR-222-5p were conducted. Cell viability and apoptosis of microglial cells were assessed via CCK8 assay and flow cytometry, respectively. Adult Sprague-Dawley (SD) rats were randomly divided into four groups: Control, SCI, sh-NC, and sh-LEF-AS1 groups. ELISA test was used to determine the expression of TNF-α and IL-6, whereas the protein level of apoptotic-related markers (Bcl-2, Bax, and cleaved caspase-3) was assessed using Western blot technique. Results We revealed that LncRNA LEF1-AS1 was distinctly upregulated, whereas miR-222-5p was significantly downregulated in LPS-treated SCI and microglial cells. However, LEF1-AS1 knockdown enhanced cell viability, inhibited apoptosis, as well as inflammation of LPS-mediated microglial cells. On the contrary, miR-222-5p upregulation decreased cell viability, promoted apoptosis, and inflammation of microglial cells. Mechanistically, LEF1-AS1 served as a competitive endogenous RNA (ceRNA) by sponging miR-222-5p, targeting RAMP3. RAMP3 overexpression attenuated LEF1-AS1-mediated protective effects on LPS-mediated microglial cells from apoptosis and inflammation. Conclusion In summary, these findings ascertain that knockdown of LEF1-AS1 impedes SCI progression via the miR-222-5p/RAMP3 axis.

scholarly journals Experimental study on the protective effects of edaravone against ischemic spinal cord injury

2005 ◽  
Vol 130 (6) ◽  
pp. 1586-1592 ◽  
Author(s):  
Kazuchika Suzuki ◽  
Teruhisa Kazui ◽  
Hitoshi Terada ◽  
Kazuo Umemura ◽  
Yasuhiko Ikeda ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Experimental Study ◽  
Spinal Cord Injury ◽  
Protective Effects ◽  
Cord Injury ◽  
Ischemic Spinal Cord Injury

scholarly journals Protective effects of glucosamine-kynurenic acid after compression-induced spinal cord injury in the rat

2012 ◽  
Vol 7 (6) ◽  
pp. 996-1004 ◽  
Author(s):  
Andrea Korimová ◽  
Dáša Cížková ◽  
Jozsef Toldi ◽  
László Vécsei ◽  
Ivo Vanický
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Amino Acid ◽  
Kynurenic Acid ◽  
Excitatory Amino Acid ◽  
Protective Effects ◽  
Tissue Destruction ◽  
Amino Acid Receptors ◽  
Cord Injury ◽  
Secondary Tissue

AbstractKynurenic acid (KYNA), a metabolite of the essential amino acid L-tryptophan, is a broad spectrum antagonist of excitatory amino acid receptors, which have also anticonvulsant and neuroprotective properties. After spinal cord injury (SCI), excitotoxicity is considered to play a significant role in the processes of secondary tissue destruction in both grey and white matter of the spinal cord. In this study, we have tested the potential therapeutic effect of glucosamine-kynurenic acid, administered after experimental compression-induced SCI in the rat. Spinal application of glucosamine-kynurenic acid continually for 24 hr after experimental SCI resulted in improved motor function recovery, beginning from the first week of evaluation and continuing until the end of the study (4 weeks). After 4 weeks’ survival, quantitative morphometric analysis of the spinal cord showed that glucosamine-kynurenic acid treatment was associated with improved tissue preservation at the lesion site. These findings indicate that spinal application of glucosaminekynurenic acid is neuroprotective and improves the outcome even when administered after spinal trauma. Our results suggest that the treatments initiated in early posttraumatic period can alleviate secondary injury and improve the final outcome after SCI.

scholarly journals Methylprednisolone Induces Neuro-Protective Effects via the Inhibition of A1 Astrocyte Activation in Traumatic Spinal Cord Injury Mouse Models

2021 ◽  
Vol 15 ◽  
Author(s):  
Hong-jun Zou ◽  
Shi-Wu Guo ◽  
Lin Zhu ◽  
Xu Xu ◽  
Jin-bo Liu
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Mouse Models ◽  
Neuronal Cells ◽  
Neurological Function ◽  
Traumatic Spinal Cord Injury ◽  
Protective Effects ◽  
Astrocyte Activation ◽  
Cord Injury

Traumatic spinal cord injury (TSCI) leads to pathological changes such as inflammation, edema, and neuronal apoptosis. Methylprednisolone (MP) is a glucocorticoid that has a variety of beneficial effects, including decreasing inflammation and ischemic reaction, as well as inhibiting lipid peroxidation. However, the efficacy and mechanism of MP in TSCI therapy is yet to be deciphered. In the present study, MP significantly attenuated the apoptotic effects of H2O2 in neuronal cells. Western blot analysis demonstrated that the levels of apoptotic related proteins, Bax and cleaved caspase-3, were reduced while levels of anti-apoptotic Bcl-2 were increased. In vivo TUNEL assays further demonstrated that MP effectively protected neuronal cells from apoptosis after TSCI, and was consistent with in vitro studies. Furthermore, we demonstrated that MP could decrease expression levels of IBA1, Il-1α, TNFα, and C3 and suppress A1 neurotoxic reactive astrocyte activation in TSCI mouse models. Neurological function was evaluated using the Basso Mouse Scale (BMS) and Footprint Test. Results demonstrated that the neurological function of MP-treated injured mice was significantly increased. In conclusion, our study demonstrated that MP could attenuate astrocyte cell death, decrease microglia activation, suppress A1 astrocytes activation, and promote functional recovery after acute TSCI in mouse models.

Morphine increases macrophages at the lesion site following spinal cord injury: Protective effects of minocycline

2019 ◽  
Vol 79 ◽  
pp. 125-138 ◽  
Author(s):  
Miriam Aceves ◽  
Mabel N. Terminel ◽  
Andre Okoreeh ◽  
Alejandro R. Aceves ◽  
Yan Ming Gong ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Protective Effects ◽  
Lesion Site ◽  
Cord Injury

scholarly journals Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats

Spinal Cord ◽  
2006 ◽  
Vol 44 (11) ◽  
pp. 662-667 ◽  
Author(s):  
Q Ao ◽  
X-H Sun ◽  
A-J Wang ◽  
P-F Fu ◽  
K Gong ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Ginkgo Biloba ◽  
Nerve Cells ◽  
Protective Effects ◽  
Egb 761 ◽  
Cord Injury

scholarly journals Protective effects of oral creatine supplementation on spinal cord injury in rats

Spinal Cord ◽  
2002 ◽  
Vol 40 (9) ◽  
pp. 449-456 ◽  
Author(s):  
ON Hausmann ◽  
K Fouad ◽  
T Wallimann ◽  
ME Schwab
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Creatine Supplementation ◽  
Protective Effects ◽  
Cord Injury
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