Solid-Phase Fluorimetric Determination of Tetracyclines in Medicinal Preparations on Cellulose Paper and in Thin-Layer Silica Gel Using a Smartphone

2021 ◽  
Author(s):  
V. G. Amelin ◽  
Z. A. C. Shogah ◽  
D. S. Bol’shakov
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Solid Phase ◽  
Fluorimetric Determination ◽  
Cellulose Paper ◽  
Medicinal Preparations

scholarly journals Photoacoustic Determination of Cobalt after Solid-Phase Concentration on an Empore Silica-gel Thin-Layer Plate.

1992 ◽  
Vol 8 (5) ◽  
pp. 655-658 ◽  
Author(s):  
Katsumi UCHIYAMA ◽  
Keiko OHSAWA ◽  
Yoshihiro YOSHIMURA ◽  
Junichi MINOWA ◽  
Takaho WATANABE ◽  
...  
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Solid Phase ◽  
Thin Layer Plate ◽  
Phase Concentration ◽  
Solid Phase Concentration

scholarly journals The Solid Phase Extraction of Some Metal Ions Using Palladium Nanoparticles Attached to Silica Gel Chemically Bonded by Silica-Bonded N-Propylmorpholine as New Sorbent prior to Their Determination by Flame Atomic Absorption Spectroscopy

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
M. Ghaedi ◽  
M. Rezakhani ◽  
S. Khodadoust ◽  
K. Niknam ◽  
M. Soylak
Keyword(s):  
Electron Microscopy ◽  
Metal Ions ◽  
Silica Gel ◽  
Solid Phase ◽  
Food Samples ◽  
Natural Food ◽  
Modified Silica Gel ◽  
Flame Atomic Absorption ◽  
Relative Standard

In this research at first palladium nanoparticle attached to a new chemically bonded silica gel has been synthesized and has been characterized with different techniques such as X-ray diffraction (XRD), fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Then, this new sorbent (chemically modified silica gel with N-propylmorpholine (PNP-SBNPM)) was efficiently used for preconcentration of some metal ions in various food samples. The influence of effective variables including mass of sorbent, flow rate, pH of sample solutions and condition of eluent such as volume, type and concentration on the recoveries of understudy metal ions were investigated. Following the optimization of variables, the interfering effects of some foreign ions on the preconcentration and determination of the investigated metal ions described. At optimum values of variables, all investigated metal ions were efficiently recovered with efficiency more than 95%, relative standard deviation (RSD) between 2.4 and 2.8, and detection limit in the range of 1.4–2.7 ng mL−1. The present method is simple and rapidly applicable for the determination of the understudied metal ions (ng mL−1) in different natural food samples.

Thin Layer Chromatographic Determination of Subsidiary Dyes in D&C Red No. 19 and D&C Red No. 37

1974 ◽  
Vol 57 (4) ◽  
pp. 961-962
Author(s):  
Sandra Bell
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Method ◽  
Chromatographic Determination

Abstract A thin layer chromatographic method is presented by which triethylrhodamine and other lower ethylated subsidiary colors are separated from D&C Red No. 19 and D&C Red No. 37. After removal from the plate, subsidiary dyes are extracted from silica gel C adsorbent and quantitated spectrophotometrically. Recoveries of 1, 2, and 5% of added triethylrhodamine ranged from 89 to 1 0 3% for D&C Red No. 19 and from 85 to 1 0 2% for D&C Red No. 37.

Thin Layer Chromatographic Determination of Citrinin

1979 ◽  
Vol 62 (1) ◽  
pp. 201-202 ◽  
Author(s):  
Robert D Stubblefield
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Linear Relationship ◽  
Silica Gel ◽  
Coefficient Of Variation ◽  
Ethylenediaminetetraacetic Acid ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Tlc Plates

Abstract Clearly defined zones of citrinin can be obtained on thin layer chromatographic (TLC) plates and measured by fluorodensitometry. Silica gel plates were prepared as a slurry with aqueous 0.05M Na2EDTA (ethylenediaminetetraacetic acid), spread at 0.5 mm wet thickness, and activated at 105°C for 1 hr. Plates were developed in acetic acid-benzene (5+95). The limit of detection was 10 ng citrinin/zone. Densitometric analysis (365 nm excitation, 505 nm emission) revealed that a linear relationship exists for levels of 10 ng to at least 100 ng/zone wtih a coefficient of variation of ±5%.

scholarly journals Identification and Quantitation of Polymyxin B, Framycetin, and Dexamethasone in an Ointment by Using Thin-Layer Chromatography with Densitometry

2005 ◽  
Vol 88 (5) ◽  
pp. 1549-1554 ◽  
Author(s):  
Jan Krzek ◽  
Anna Maślanka ◽  
Pawel Lipner
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Polymyxin B ◽  
Rapid Identification ◽  
Mobile Phases ◽  
Relative Standard ◽  
Dexamethasone Acetate ◽  
Similar Accuracy ◽  
Layer Chromatography

Abstract A new thin-layer chromatographic-densitometric method has been developed for rapid identification and quantitative determination of polymyxin B, framycetin, and dexamethasone in a dental ointment. Silica gel 60 and F254 silica gel 60 plates were used for separating antibiotics and dexamethasone acetate, respectively. When determining framycetin and polymyxin B, chromatograms were developed by using 2 mobile phases, namely methanol and methanol–n-butanol–ammonia (25%)–chloroform (14 + 4 + 9 + 12, v/v/v/v/). The densitometric measurements were made at 550 nm after detection with 0.3% ninhydrin solution. Dexamethasone was determined by using the mobile phase cyclohexane–ethyl acetate (2 + 3, v/v) and ultraviolet densitometric recording at 245 nm. The results obtained for individual constituents with the chromatographic-densitometric method demonstrate similar accuracy, relative standard deviation values from 1.49 to 2.47%, and relative error values from 0.02 to 0.81% and are comparable to those obtained with the reference methods.

Quantitative Determination of Tris-(2,3,-dibromopropyl) Phosphate by Densitometry of Thin Layer Chromatograms

1979 ◽  
Vol 62 (6) ◽  
pp. 1315-1318
Author(s):  
Albert M Gardner
Keyword(s):  
Sewage Sludge ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Ethyl Acetate ◽  
Silver Nitrate ◽  
Flame Retardant ◽  
Silica Gel ◽  
White Background ◽  
Ppm Level

Abstract The flame retardant tris-(2,3-dibromopropyl) phosphate (Tris) was chromatographed on silica gel thin layer plates, using ethyl acetate-hexane (30+70) as the developing solvent. Tris was visualized by spraying the chromatogram with 1% aqueous silver nitrate followed by exposure to ultraviolet light for 40 min. Tris appeared as dark gray spots on a white background; the spots were quantitated by densitometry at 600 nm. The lower level of sensitivity was 50 ng; calibration plots were linear from 50 to 800 ng. The procedure was developed to determine the levels of unmetabolized Tris remaining after exposure to sewage sludge suspensions. Recovery of Tris from 3 sewage sludge samples fortified at the 1.0 ppm level averaged 97%.

scholarly journals Spectrophotometric Determination of Iron(II) after Solid Phase Extraction of Its 2,2′ Bipyridine Complex on Silica Gel-Polyethylene Glycol

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Nahid Pourreza ◽  
Saadat Rastegarzadeh ◽  
Ali Reza Kiasat ◽  
Hossein Yahyavi
Keyword(s):  
Polyethylene Glycol ◽  
Solid Phase Extraction ◽  
Silica Gel ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Calibration Graph ◽  
Phase Extraction ◽  
Relative Standard

A new solid phase extraction procedure was developed for preconcentration of iron(II) using silica gel-polyethylene glycol (silica-PEG) as an adsorbent. The method is based on retention of iron(II) as 2,2′ bipyridine complex on silica-PEG. The retained complex is eluted by 1.0 mol L−1of sulfuric acid-acetone mixture (1:2) and its absorbance is measured at 518 nm, spectrophotometrically. The effects of different parameters such as pH, concentration of the reagent, eluting reagent, sample volume, amount of adsorbent, and interfering ions were investigated. The calibration graph was linear in the range of 1–60 ng mL−1of iron(II). The limit of detection based on3Sbwas 0.57 ng mL−1and relative standard deviations (R.S.D) for ten replicate measurements of 12 and 42 ng mL−1of iron(II) were 2.4 and 1.7%, respectively. The method was applied to the determination of of iron(II) in water, multivitamin tablet, and spinach samples.

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