Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling

2008 ◽  
Vol 46 (4) ◽  
Author(s):  
W. H. Hu ◽  
X. S. Song ◽  
K. Shi ◽  
X. J. Xia ◽  
Y. H. Zhou ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Electron Transport ◽  
Ascorbate Peroxidase ◽  
Peroxidase Isoenzymes

scholarly journals Morphological and Biochemical Alterations of Paddy Rice in Response to Stress Caused by Herbicides and Total Plant Submersion

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
M.G. MARCHEZAN ◽  
L.A. AVILA ◽  
D. AGOSTINETTO ◽  
C.E. SCHAEDLER ◽  
A.C. LANGARO ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Ascorbate Peroxidase ◽  
Paddy Rice ◽  
Total Phenolic ◽  
Total Chlorophyll ◽  
Water Regimes ◽  
Biochemical Alterations ◽  
Response To Stress ◽  
Total Plant ◽  
Induced Changes

ABSTRACT Herbicide selectivity in paddy rice varies in several aspects, among which are the environmental conditions. The aim of the study was to evaluate the effect of herbicide application and total plant submersion on morphological and biochemical changes in paddy rice. Total chlorophyll and carotenoids, catalase activity, ascorbate peroxidase and superoxide dismutase, total phenolic content, lipid peroxidation and hydrogen peroxide levels were assessed. Leaf samples were collected 24 hours and 7 days after the application of water regimes. The results observed in the first experiment show that cultivars Puitá INTA CL, IRGA 417 and IRGA 422 CL are more tolerant to total submersion. The most sensitive cultivars are IRGA 424, BRS Querência, EPAGRI 108 and BRS Taim. In general, cultivar Puitá INTA CL had lower oxidative damage than BRS Querência when under submersion. To eliminate excess free radicals, BRS Querência had increased activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) than Puitá INTA CL under submersion. Formulations with imazethapyr + imazapic and imazapyr + imazapic caused greater reduction in the total chlorophyll and carotenoid contents at 7 days after the establisment of water regimes (DAT). Therefore, the data show that exposing cultivars to total submersion and herbicides increased oxidative stress as well as induced changes in the activities of antioxidant enzymes.

scholarly journals Comparison of defence responses to Botrytis cinerea infection in tomato plants propagated in vitro and grown in vivo

2013 ◽  
Vol 49 (1-2) ◽  
pp. 115-121 ◽  
Author(s):  
Jacek Patykowski ◽  
Elżbieta Kuźniak ◽  
Henryk Urbaniak
Keyword(s):  
Superoxide Dismutase ◽  
Botrytis Cinerea ◽  
Ascorbate Peroxidase ◽  
Guaiacol Peroxidase ◽  
Activity Increase ◽  
Tomato Plants ◽  
Defence Responses ◽  
Defence Reactions

Defence reactions: O<sub>2<sub> - generation, superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase activities after <em>B. cinerea</em> infection in tomato plants propagated <em>in vitro</em> and grown <em>in vivo</em> have been compared. Infection resulted in rapid O<sub>2<sub> - generation. Superoxide dismutase activity increase was slower than O<sub>2<sub> - response. In plants propagated <em>in vitro</em> catalase and guaiacol peroxidase activities after infection were induced less strongly than in plants grown <em>in vivo</em>. K<sub>2<sub>HPO<sub>4<sub> pretreatment of plants grown <em>in vitro</em> enhanced significantly the activities of catalase and guaiacol peroxidase after infection. Slight restriction of <em>B. cinerea</em> infection development in <em>in vitro</em> propagated plants pretreated with K<sub>2<sub>HP0<sub>4<sub> was observed.

Pulmonary reduction of an intravascular redox polymer

2001 ◽  
Vol 280 (6) ◽  
pp. L1290-L1299 ◽  
Author(s):  
Said H. Audi ◽  
Robert D. Bongard ◽  
Yoshiyuki Okamoto ◽  
Marilyn P. Merker ◽  
David L. Roerig ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Superoxide Dismutase ◽  
Electron Transport ◽  
Kinetic Model ◽  
Electron Acceptors ◽  
Redox Polymer ◽  
Pulmonary Endothelial Cells ◽  
Equilibrium Level ◽  
A Cell

Pulmonary endothelial cells in culture reduce external electron acceptors via transplasma membrane electron transport (TPMET). In studying endothelial TPMET in intact lungs, it is difficult to exclude intracellular reduction and reducing agents released by the lung. Therefore, we evaluated the role of endothelial TPMET in the reduction of a cell-impermeant redox polymer, toluidine blue O polyacrylamide (TBOP+), in intact rat lungs. When added to the perfusate recirculating through the lungs, the venous effluent TBOP+concentration decreased to an equilibrium level reflecting TBOP+ reduction and autooxidation of its reduced (TBOPH) form. Adding superoxide dismutase (SOD) to the perfusate increased the equilibrium TBOP+ concentration. Kinetic analysis indicated that the SOD effect could be attributed to elimination of the superoxide product of TBOPH autooxidation rather than of superoxide released by the lungs, and experiments with lung-conditioned perfusate excluded release of other TBOP+ reductants in sufficient quantities to cause significant TBOP+ reduction. Thus the results indicate that TBOP+ reduction is via TPMET and support the utility of TBOP+ and the kinetic model for investigating TPMET mechanisms and their adaptations to physiological and pathophysiological stresses in the intact lung.

Photosynthetic Electron Transport Regulates the Expression of Cytosolic Ascorbate Peroxidase Genes in Arabidopsis during Excess Light Stress

1997 ◽  
Vol 9 (4) ◽  
pp. 627 ◽  
Author(s):  
Stanislaw Karpinski ◽  
Carolina Escobar ◽  
Barbara Karpinska ◽  
Gary Creissen ◽  
Philip M. Mullineaux
Keyword(s):  
Electron Transport ◽  
Ascorbate Peroxidase ◽  
Light Stress ◽  
Photosynthetic Electron Transport ◽  
Cytosolic Ascorbate Peroxidase ◽  
Excess Light

Relationship between Activities of Enzymes for the Removal of O2·- and H2O2 and Nitrogenase in Root Nodules of Phaseolus vulgaris L.

1995 ◽  
Vol 50 (7-8) ◽  
pp. 543-551
Author(s):  
Bernhard Epping ◽  
Alexander P. Hansen ◽  
Peter Martin
Keyword(s):  
Superoxide Dismutase ◽  
Phaseolus Vulgaris ◽  
Ascorbate Peroxidase ◽  
Peroxidase Activity ◽  
Rhizobium Leguminosarum ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Hypersensitive Reaction ◽  
Guaiacol Peroxidase ◽  
Bacterial Strains

Abstract Nodules of Rhizobium leguminosarum bv. phaseoli in symbiosis with Phaseolus vulgaris were compared with regard to their nitrogenase activity and activities of enzymes involved in the removal of O2·- and H2O2 as well as total ascorbate content. Activities of catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11), and total ascorbate content were consist­ently higher in nodules inhabited by bacterial strains with higher nitrogenase activity. Values for superoxide dismutase (EC 1.15.11), and guaiacol peroxidase activity did not differ for the bacterial strains compared. On the other hand, when different plant cultivars were inoculated with the same bacterial strain, high nitrogenase activity did not correlate with a higher activ­ity of the oxygen scavenging enzyms or a higher content of total ascorbate. In this case, values for guaiacol peroxidase activity were greatly enhanced in nodules with lower nitrogen­ ase activity. This may be part of a hypersensitive reaction of the plant cultivar against the bacterial symbiotic partner. Inhibition of catalase activity in the nodules by addition of triazole to the nutrient solution did not alter nitrogenase activity within the first nine hours after addition. It can be concluded that the activity of catalase, ascorbate peroxidase, and superoxide dismutase is not generally coupled to nitrogenase activity in root nodules of P. vulgaris.

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