scholarly journals Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea

2019 ◽  
Vol 101 (1-2) ◽  
pp. 65-79 ◽  
Author(s):  
Diana L. Zuluaga ◽  
Neil S. Graham ◽  
Annett Klinder ◽  
A. E. Elaine van Ommen Kloeke ◽  
Angelo R. Marcotrigiano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Oleracea ◽  
Aliphatic Glucosinolate

Alfin-like transcription factor family: characterization and expression profiling against stresses in Brassica oleracea

2016 ◽  
Vol 38 (5) ◽  
Author(s):  
Md. Abdul Kayum ◽  
Jong-In Park ◽  
Nasar Uddin Ahmed ◽  
Gopal Saha ◽  
Mi-Young Chung ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Oleracea ◽  
Expression Profiling ◽  
Transcription Factor Family

scholarly journals An ERF transcription factor from Brassica oleracea: a new member of the emerging pathogenicity hub in plant-Xanthomonas interactions

2020 ◽  
Author(s):  
Nikolay Zlobin ◽  
Marina Lebedeva ◽  
Yuliya Monakhova ◽  
Vera Ustinova ◽  
Vasiliy Taranov
Keyword(s):  
Transcription Factor ◽  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Target Genes ◽  
Computational Prediction ◽  
Amplicon Sequencing ◽  
List Type ◽  
Tal Effectors ◽  
Xanthomonas Species ◽  
Rot Disease

Summary- TAL effectors (TALEs), which induce the expression of specific plant genes to promote infection, are the main pathogenic determinants of different Xanthomonas bacteria. However, investigation of TALEs from Xanthomonas campestris pv. campestris, which causes black rot disease of crucifers, is in its infancy.- In this study, we used PCR-based amplification in conjunction with SMRT amplicon sequencing to identify TALE genes in several Xanthomonas campestris pv. campestris strains and performed computational prediction in conjunction with RT-PCR-based analysis to identify their target genes in Brassica oleracea.- Transcription factor from the AP2/ERF family was predicted to be putative target gene for the conserved TALEs present in multiple Xanthomonas campestris pv. campestris strains. Its expression dramatically increased upon leaf inoculation with strains harbouring such TALEs.- Several members of the AP2/ERF factor family from different plant species were identified as targets of TALEs from various Xanthomonas species, which suggests that they constitute a new pathogenicity hub in plant-Xanthomonas interactions.

scholarly journals The Effect of Introgression of an R2R3 MYB Transcription Factor on Sulphur Metabolism in Brassica oleracea

2020 ◽  
Author(s):  
Mikhaela Neequaye ◽  
Shikha Saha ◽  
Martin Trick ◽  
Burkhard Steuernagel ◽  
Perla Troncoso-Rey ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Oleracea ◽  
Genetic Variant ◽  
Myb Transcription Factor ◽  
Sulphur Metabolism ◽  
Expression Of Genes ◽  
Downstream Effects ◽  
Sulphate Assimilation ◽  
Enhanced Expression ◽  
R2r3 Myb

AbstractBackgroundA diet rich in cruciferous vegetables is reported to have beneficial health effects, partially mediated by 4-methylsulfinylbutyl glucosinolate, or glucoraphanin, which is predominantly found within broccoli (Brassica oleracea var italica). We describe the downstream effects on transcription and metabolism in broccoli following the introgression of a genetic variant of MYB28 into broccoli from a wild Brassica relative which has previously been associated with enhancement of glucoraphanin.ResultsWhole genome sequencing, RNA expression and metabolite analyses were used to characterise the consequences of the introgression of either one or two copies of a genetic variant of the MYB28 transcription factor into a commercial broccoli genetic background. The introgression of the variant of MYB28 resulted in enhanced expression of genes involved in primary sulphate assimilation, sulphur metabolism and aliphatic glucosinolate biosynthesis, and enhanced accumulation of 4-methylsulphinyl butyl glucosinolate in florets. Other changes in transcription that may be related to non-targeted introgression events are reported. There were no consistent effects upon sulphur metabolites pools, apart from methionine-derived glucosinolates.ConclusionThis study illustrates the downstream effects on transcription and metabolism of the introgression of a genetic variant of MYB28 from a wild species into a commercial broccoli genotype.

scholarly journals Epistatic Transcription Factor Networks Differentially Modulate Arabidopsis Growth and Defense

2019 ◽  
Author(s):  
Baohua Li ◽  
Michelle Tang ◽  
Céline Caseys ◽  
Ayla Nelson ◽  
Marium Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Energy Costs ◽  
Simple Correlation ◽  
Negative Interaction ◽  
Coordination Model ◽  
High Throughput Phenotyping ◽  
Transcription Factor Networks ◽  
External Signals ◽  
The Relationship ◽  
Aliphatic Glucosinolate

AbstractPlants integrate internal and external signals to finely coordinate growth and defense allowing for maximal fitness within a complex environment. One common model for the relationship between growth and defense is a trade-off model in which there is a simple negative interaction between growth and defense theoretically driven by energy costs. However, there is a developing consensus that the coordination of growth and defense likely involves a more conditional and intricate connection. To explore how a transcription factor network may coordinate growth and defense, we used high-throughput phenotyping to measure growth and flowering in a set of single and pairwise mutants previously linked to the aliphatic glucosinolate defense pathway. Showing the link between growth and aliphatic glucosinolate defense, 17 of the 20 tested TFs significantly influence plant growth and/or flowering time. These effects were conditional upon the environment, age of the plant and more critically varied amongst the phenotypes when using the same genotype. The phenotypic effects of the TF mutants on SC GLS accumulation and on growth did not display a simple correlation, supporting the coordination model. We propose that large transcription factor networks create a system to integrate internal and external signals and separately modulate growth and the accumulation of the defensive aliphatic GLS.

scholarly journals Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots

2020 ◽  
Vol 21 (16) ◽  
pp. 5721
Author(s):  
Ji-Nam Kang ◽  
So Youn Won ◽  
Mi-Suk Seo ◽  
Jeongyeo Lee ◽  
Si Myung Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcriptome Analysis ◽  
Raphanus Sativus ◽  
Biosynthesis Pathway ◽  
Comparative Transcriptome ◽  
Qrt Pcr ◽  
Raphanus Sativus L ◽  
Significant Expression ◽  
Aliphatic Glucosinolate

Glucoraphasatin (GRH) is a specific aliphatic glucosinolate (GSL) that is only abundant in radish (Raphanus sativus L.). The gene expression regulating GRH biosynthesis in radish is still poorly understood. We employed a total of 59 radish accessions to analyze GSL profiles and showed that GRH was specific and predominant among the aliphatic GSLs in radish roots. We selected five accessions roots with high, moderate and low GSL biosynthesis, respectively, to conduct a comparative transcriptome analysis and the qRT-PCR of the biosynthesis genes for aliphatic GSLs. In this study, among all the accessions tested, roots with the accession RA157-74 had a high GRH content and showed a significant expression of the aliphatic GSL biosynthesis genes. We defined the genes involved in the GRH biosynthesis process and found that they were regulated by a transcription factor (RSG00789) at the MYB29 locus in radish roots. We found 13 aliphatic GSL biosynthesis genes regulated by the RSG00789 gene in the GRH biosynthesis pathway.

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