scholarly journals In vitro propagation, DNA content and essential oil composition of Teucrium scorodonia L. ssp. scorodonia

2016 ◽  
Vol 127 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Joanna Makowczyńska ◽  
Elwira Sliwinska ◽  
Danuta Kalemba ◽  
Ewelina Piątczak ◽  
Halina Wysokińska
Keyword(s):  
Essential Oil ◽  
Dna Content ◽  
In Vitro Propagation ◽  
Essential Oil Composition ◽  
Oil Composition

Investigation of the Essential Oil of Ormenis africana from Morocco: Revision of Chemical Composition and Antibacterial Activity

2021 ◽  
Vol 1 (30) ◽  
pp. 34-38
Author(s):  
El Hanbali F Barrero A.F
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Proteus Vulgaris ◽  
Oil Composition ◽  
Gram Negative ◽  
Main Compound ◽  
Oxygenated Compounds ◽  
Aerial Parts

Abstract- The essential oil composition from the aerial parts of Ormenis africana (Asteraceae), an endemic species from Morocco, has been investigated by GC/MS. A total of 31 compounds were identified, representing 77%. After fractionation by column chromatography, the main compound was isolated and its structure elucidated by NMR spectroscopy. The essential oil was dominated by oxygenated compounds with spathulenol (45.8%) followed by camphor (7.1%), -cadinol (5.9%) and -bisabolol (5.9%) as the main compounds. This oil can be classified as spathulenol-type according to its spathulenol content. In vitro the antibacterial activity of the whole essential oil against three Gram positive (Bacillus cereus, Enterococcus faecalis, Streptococcus C) bacteria and three Gram negative (Proteus vulgaris, Escherichia coli, Pseudomonas aeroginosa) bacteria, showed significant results. Keywords: Asteraceae, Ormenis africana, Essential oil, Spathulenol, Antibacterial activity.

scholarly journals Essential oil composition and in vitro antibacterial activity of Chenopodium album subsp. striatum

2018 ◽  
Vol 69 (2) ◽  
pp. 144-155 ◽  
Author(s):  
Negin Khomarlou ◽  
Parviz Aberoomand-Azar ◽  
Ardalan Pasdaran Lashgari ◽  
Hamid Tebyanian ◽  
Ali Hakakian ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chenopodium Album ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
In Vitro Antibacterial Activity

scholarly journals Premna odorata: Seasonal Metabolic Variation in the Essential Oil Composition of Its Leaf and Verification of Its Anti-Ageing Potential via In Vitro Assays and Molecular Modelling

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 879
Author(s):  
Ahmed E. Altyar ◽  
Mohamed L. Ashour ◽  
Fadia S. Youssef
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Molecular Modelling ◽  
Essential Oil Composition ◽  
In Vitro Assays ◽  
Oil Composition ◽  
Different Seasons ◽  
Metabolic Variation ◽  
Active Centres

The metabolic variation in the essential oil composition of Premna odorata leaves obtained from different seasons was quantitatively and qualitatively determined employing GC/MS (Gas Chromatography coupled with Mass Spectrometry) and GC/FID (Gas chromatography equipped with flame ionization detector) techniques. It displayed the existence of 97 constituents accounting for 94.19%, 92.27%, 91.95% and 92.63% for POS (spring), POM (summer), POA (autumn) and POW (winter) whole essential oils. β-Caryophyllene constituting the main metabolite in the oil in the different seasons. To better visualize the differences between them, GC data were exposed to chemometric analysis. A PCA (principal component analysis) score plot revealed the closeness of POS and POW. Molecular modelling on collagenase, elastase and hyaluronidase enzymes active centres shows that different compounds existing in the essential oil of Premna odorata leaves shows binding to the active sites with variable degrees that suggested its anti-ageing potential. Palmitic acid displayed the highest fitting for both the collagenase and elastase active centres in both pH-based and rule-based ionization methods with ∆G equals −78.27 and −44.77 kcal/mol, respectively; meanwhile, heptacosane showed the highest fitting score in the hyaluronidase centre with ∆G = −43.78 kcal/mol. In vitro assays consolidates the obtained modelling studies in which essential oil shows considerable anti-elastase and anti-hyaluronidase potential as evidenced by their IC50 values being 49.3 and 37.7 μg/mL, respectively; meanwhile, the essential oil of Premna odorata leaves displayed mild anti-collagenase potential. Thus, it can be concluded that Premna odorata could serve as a promising anti-ageing naturally occurring drug that could be effectively incorporated by pharmaceutical industries in cosmetics combating ageing and skin wrinkling.

scholarly journals Antibacterial Activity and Essential Oil Composition of Satureja spicigera from Iran

2009 ◽  
Vol 64 (1-2) ◽  
pp. 20-24 ◽  
Author(s):  
Fereshteh Eftekhar ◽  
Fereshteh Raei ◽  
Morteza Yousefzadi ◽  
Samad Nejad Ebrahimi ◽  
Javad Hadian
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Resistant Organism ◽  
Disc Diffusion ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Full Flowering

The aerial parts of Satureja spicigera were collected at full flowering stage at Gazvin, Iran. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Fourteen compounds were identified, of which carvacrol (53.74%) and thymol (36.03%) were the main constituents, representing 99.12% of the total oil. The in vitro antibacterial activity of the essential oil was determined against six ATCC standard bacterial strains (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) using disc diffusion as well as measurement of minimum inhibitory concentrations. The disc diffusion results and MIC values indicated high inhibitory activity against the test bacteria. The most susceptible organisms were the Gram-positive B. subtilis and S. aureus followed by E. faecalis, usually resistant to most common antibiotics. Among the Gram-negative bacteria, E. coli and K. pneumoniae were highly sensitive to the different oil concentrations in the disc diffusion method. Finally, P. aeruginosa, a highly resistant organism to most antibiotics, showed moderate susceptibility to Satureja spicigera essential oil.

In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species

2004 ◽  
Vol 95 (2-3) ◽  
pp. 253-258 ◽  
Author(s):  
A.C.U. Lourens ◽  
D. Reddy ◽  
K.H.C. Başer ◽  
A.M. Viljoen ◽  
S.F. Van Vuuren
Keyword(s):  
Biological Activity ◽  
Essential Oil ◽  
South African ◽  
Essential Oil Composition ◽  
Oil Composition

scholarly journals Antifungal Activity of the Essential Oils from Ocimum gratissimum L. Grown in Togo

2008 ◽  
Vol 1 (1) ◽  
pp. 164-171 ◽  
Author(s):  
Koffi Koba ◽  
P W Poutouli ◽  
Christine Raynaud ◽  
Komla Sanda
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Filamentous Fungi ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Minimum Fungicidal Concentration ◽  
Ocimum Gratissimum ◽  
Aerial Parts ◽  
In Vitro Antifungal Activity

The aerial parts of Ocimum gratissimum L. (Lamiaceae) harvested in Togo was steam-distilled and investigated for essential oil composition (GC and GC/MS) and in vitro antifungal activities. Thymol (31.79 %), p-cymene (15.57 %) and γ-terpinene (12.34 %) and were the major components of the oil. Other notable components identified in this oil were myrcene (6.94 %) and α-thujene (6.11 %).The in vitro antifungal activity was recorded with the minimum inhibitory concentrations (MICs) ranging from 80 to 150 µl.l-1, 150 to 500 µl.l-1  and from 100 to 150 µl.l-1 respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Likewise, on tested fungi the minimum fungicidal concentration (MFC) varied from 300 µl.l-1 to 500 µl.l-1, 500 to 700 µl.l-1 and from 250 to 300 µl.l-1, respectively on dermatophytes, imperfect filamentous fungi and pathogenic yeasts. Keywords: O.gratissimum,  Antifungal, Essential oil; Thymol. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1131 

scholarly journals Essential Oil Composition of Two Sphagnum Species Grown in Portugal and their In Vitro Culture Establishment

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Manuela Sim-Sim ◽  
Margarida Abreu ◽  
César Garcia ◽  
Cecília Sérgio ◽  
A. Cristina Figueiredo
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
In Vitro Culture ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Peat Moss ◽  
Oil Composition ◽  
Moss Species

Two peat moss species, frequent both in the mainland Portugal and in the Azores archipelago, were evaluated for essential oil composition and establishment under in vitro culture. Sphagnum auriculatum and Sphagnum subnitens essential oils were isolated by hydrodistillation and analysed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS). The essential oil of S. auriculatum was dominated by an as yet unidentified sesquiterpene, whereas zierene was the main component of S. subnitens essential oil. The in vitro cultures were successfully established for future studies of their chemical profile. The components present in essential oils obtained from S. auriculatum and S. subnitens together with morphological traits could be used to support the taxonomy of this plant group.

scholarly journals Anti-Helicobacter pylori activity and Essential Oil Composition of Thymus caramanicus from Iran

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Fereshteh Eftekhar ◽  
Farahnaz Nariman ◽  
Morteza Yousefzadi ◽  
Javad Hadian ◽  
Samad Nejad Ebrahimi
Keyword(s):  
Helicobacter Pylori ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Oil Composition ◽  
Full Flowering ◽  
Main Component

The essential oil of the dried aerial parts of Thymus caramanicus at full flowering stage was prepared by hydrodistillation with a yield of 2.5% oil. The oil analysis by a combination of capillary GC and GC-MS revealed 26 components of which carvacrol (68.9%) was the main component, followed by p-cymene (6.0%), thymol (5.3%), γ-terpinene (4.6%) and borneol (4.0%) representing 98.9% of the total oil. The in vitro antibacterial activity of the essential oil was determined against ten clinical isolates of Helicobacter pylori using disc diffusion, as well as measurement of minimum inhibitory concentrations. The results showed high inhibitory activity against all test bacteria by the disc diffusion method (zones of inhibition of 50.0 – 65.0 mm). Minimum inhibitory concentration values were within the range 14.5 – 58.0 μg/mL for the clinical isolates.

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