scholarly journals Antibacterial Activity and Essential Oil Composition of Satureja spicigera from Iran

2009 ◽  
Vol 64 (1-2) ◽  
pp. 20-24 ◽  
Author(s):  
Fereshteh Eftekhar ◽  
Fereshteh Raei ◽  
Morteza Yousefzadi ◽  
Samad Nejad Ebrahimi ◽  
Javad Hadian
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Resistant Organism ◽  
Disc Diffusion ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Full Flowering

The aerial parts of Satureja spicigera were collected at full flowering stage at Gazvin, Iran. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Fourteen compounds were identified, of which carvacrol (53.74%) and thymol (36.03%) were the main constituents, representing 99.12% of the total oil. The in vitro antibacterial activity of the essential oil was determined against six ATCC standard bacterial strains (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) using disc diffusion as well as measurement of minimum inhibitory concentrations. The disc diffusion results and MIC values indicated high inhibitory activity against the test bacteria. The most susceptible organisms were the Gram-positive B. subtilis and S. aureus followed by E. faecalis, usually resistant to most common antibiotics. Among the Gram-negative bacteria, E. coli and K. pneumoniae were highly sensitive to the different oil concentrations in the disc diffusion method. Finally, P. aeruginosa, a highly resistant organism to most antibiotics, showed moderate susceptibility to Satureja spicigera essential oil.

scholarly journals Anti-Helicobacter pylori activity and Essential Oil Composition of Thymus caramanicus from Iran

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Fereshteh Eftekhar ◽  
Farahnaz Nariman ◽  
Morteza Yousefzadi ◽  
Javad Hadian ◽  
Samad Nejad Ebrahimi
Keyword(s):  
Helicobacter Pylori ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Oil Composition ◽  
Full Flowering ◽  
Main Component

The essential oil of the dried aerial parts of Thymus caramanicus at full flowering stage was prepared by hydrodistillation with a yield of 2.5% oil. The oil analysis by a combination of capillary GC and GC-MS revealed 26 components of which carvacrol (68.9%) was the main component, followed by p-cymene (6.0%), thymol (5.3%), γ-terpinene (4.6%) and borneol (4.0%) representing 98.9% of the total oil. The in vitro antibacterial activity of the essential oil was determined against ten clinical isolates of Helicobacter pylori using disc diffusion, as well as measurement of minimum inhibitory concentrations. The results showed high inhibitory activity against all test bacteria by the disc diffusion method (zones of inhibition of 50.0 – 65.0 mm). Minimum inhibitory concentration values were within the range 14.5 – 58.0 μg/mL for the clinical isolates.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Retrohpyllum rospigliosii Fruits from Colombia

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Clara E. Quijano-Celis ◽  
Mauricio Gaviria ◽  
Consuelo Vanegas-López ◽  
Ina Ontiveros ◽  
Leonardo Echeverri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Positive Bacterium ◽  
Disc Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
Bacterium Bacillus

The essential oil from fruits of Retrophyllum rospigliosii (Pilger) C.N. Page grown in Colombia was analyzed by GC and GC/MS. Ninety-one compounds were identified, of which the most prominent were limonene (37.7%) and α-pinene (16.3%). The in vitro antibacterial activity of the essential oil was studied against seven bacterial strains using the disc diffusion method. The strongest activity of the oil was against the Gram-positive bacterium Bacillus cereus.

Investigation of the Essential Oil of Ormenis africana from Morocco: Revision of Chemical Composition and Antibacterial Activity

2021 ◽  
Vol 1 (30) ◽  
pp. 34-38
Author(s):  
El Hanbali F Barrero A.F
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Proteus Vulgaris ◽  
Oil Composition ◽  
Gram Negative ◽  
Main Compound ◽  
Oxygenated Compounds ◽  
Aerial Parts

Abstract- The essential oil composition from the aerial parts of Ormenis africana (Asteraceae), an endemic species from Morocco, has been investigated by GC/MS. A total of 31 compounds were identified, representing 77%. After fractionation by column chromatography, the main compound was isolated and its structure elucidated by NMR spectroscopy. The essential oil was dominated by oxygenated compounds with spathulenol (45.8%) followed by camphor (7.1%), -cadinol (5.9%) and -bisabolol (5.9%) as the main compounds. This oil can be classified as spathulenol-type according to its spathulenol content. In vitro the antibacterial activity of the whole essential oil against three Gram positive (Bacillus cereus, Enterococcus faecalis, Streptococcus C) bacteria and three Gram negative (Proteus vulgaris, Escherichia coli, Pseudomonas aeroginosa) bacteria, showed significant results. Keywords: Asteraceae, Ormenis africana, Essential oil, Spathulenol, Antibacterial activity.

scholarly journals Essential Oil from Leaves of Lantana canescens and L. lopez-palacii Grown in Colombia

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600
Author(s):  
Andrés F. Peralta-Bohórquez ◽  
Clara Quijano-Célis ◽  
Mauricio Gaviria ◽  
Consuelo Vanegas-López ◽  
Jorge A. Pino
Keyword(s):  
Essential Oil ◽  
Volatile Compounds ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Enterobacter Sakazakii ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Germacrene D ◽  
In Vitro Antibacterial Activity

The chemical composition of the volatile compounds from the leaves of Lantana canescens Kunth (Verbenaceae) and L. lopez-palacii Moldenke grown in Colombia were analyzed by GC and GC/MS. One hundred and thirty-nine volatile compounds were identified in L. canescens, of which the major ones were β-caryophyllene (13.5%), germacrene D (10.3%) and 1-octen-3-ol (8.4%). In the oil obtained from L. lopez-palacii, eighty-three compounds were identified, of which the most prominent were 1-octen-3-ol (24.4%) and β-caryophyllene (15.2%). The in vitro antibacterial activity of the L. lopez-palacii essential oil was studied against three bacterial strains using the disc diffusion method. No antimicrobial activity was found against Escherichia coli, Enterobacter sakazakii and Listeria monocytogenes.

scholarly journals Essential oil composition and in vitro antibacterial activity of Chenopodium album subsp. striatum

2018 ◽  
Vol 69 (2) ◽  
pp. 144-155 ◽  
Author(s):  
Negin Khomarlou ◽  
Parviz Aberoomand-Azar ◽  
Ardalan Pasdaran Lashgari ◽  
Hamid Tebyanian ◽  
Ali Hakakian ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chenopodium Album ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
In Vitro Antibacterial Activity

scholarly journals CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF THE ESSENTIAL OIL OF THE Toona sureni (Blume) Merr

2015 ◽  
Vol 3 (1) ◽  
pp. 90
Author(s):  
M. Taufik Ekaprasada ◽  
Hazli Nurdin ◽  
Sanusi Ibrahim ◽  
Dachriyanus
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Germacrene D ◽  
Total Oil

 ABSTRACT The essential oil composition of the Toona sureni (Blume) Merr leaf was analyzed by GC-MS. More than 68 peaks, representing 99.99% of total oil, forty three components were identified, this represents 80.65% of the total oil component. The major components were α-terpinene (9.58%), α-copaene (8.39%), bicyclogermacrene (7.61%), δ-cadinene (6.65%), β-elemene (4.88%), germacrene-D (4.65%), δ-selinene (3.58%), caralene (3.10%), β-caryophyllene (2.88%), α-cubebene (2.82%), δ-gurjunene (2.20%), and (-)-isoledene (2.05%). The antibacterial activity of the essential oil of Toona sureni (Blume) Merr leaf was evaluated using disk diffusion method. The oil was effective on the inactivation of Escherichia coli, Staphylococcus aureus and Bacillus subtilis. Keywords: Toona sureni (Blume) Merr, antibacterial activity, GC-MS, essential oil

scholarly journals Chemical composition and antibacterial activity of Origanum ramonense essential oil on the β-lactamase and extended- spectrum β-lactamase urinary tract isolates

2018 ◽  
Vol 13 (3) ◽  
pp. 280 ◽  
Author(s):  
Haitham N. Qaralleh
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Essential Oil ◽  
Video Clip ◽  
Enterobacter Aerogenes ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Extended Spectrum ◽  
Total Oil

<p class="Abstract">The aim of this study was to evaluate the antibacterial activity of <em>Origanum ramonense</em> essential oil extracted from the air-dried leaves against β-lactamase and extended-spectrum β-lactamase obtained from the patients with urinary tract infection. The essential oil was extracted by hydrodistillation and analyzed by GC–MS. In vitro antibacterial activity was studied using disc diffusion and microdilution methods. Twenty compounds were identified representing 97.8% of the total oil. The major components were carvacrol (84.6%), p-cymene (4.3%) and γ-terpinene (3.3%). The oil showed a broad spectrum of antibacterial activity against all tested isolates. <em>Staphylococcus aureus, S. epidermidis, Klebsiella pneumoniae</em> and <em>Enterobacter aerogenes</em> had the lowest minimum inhibitory concentration values (0.015 µg/mL) followed by <em>Escherichia coli</em> (0.14 µg/mL). The lowest susceptible strains to oil were <em>Pseudomonas aeruginosa, Proteus mirabilis, E. coli</em> 25922 and <em>P. aeruginosa</em> 10145. The bacteriostatic and bactericidal effects at concentrations as low as 0.015 µg/mL indicated the potent antibacterial activity of <em>O. ramonense</em>. </p><p><strong>Video Clip of Methodology:</strong></p><p>Disc Diffusion method: 2 min 16 sec   <a href="https://www.youtube.com/v/HUZqSngRvmQ">Full Screen</a>   <a href="https://www.youtube.com/watch?v=HUZqSngRvmQ">Alternate</a></p>

scholarly journals Analysis of Chemical Components and Antibacterial Activity in Essential Oil of Lantana Flowers (Lantana Camara L)

Biomedika ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 19-28
Author(s):  
Yulia Shara Sembiring ◽  
Cut Fatimah Zuhra
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Antibacterial Activities ◽  
Lantana Camara ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Chemical Components ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Zone Of Inhibition

The purpose of this study was to evaluate the essential oil composition as well as antibacterial activities of essential oil of Lantana camara L flowers against four bacterial strains. Essential oil of Lantana camara L flowers was obtained by hydrodistillation method using Sthal and analyzed by GC-MS. The antibacterial activities of essential oil were tested by using disk diffusion method against four bacterial strains. Results showed  72 compounds for Lantana flowers essential oil, of which caryophyllene   (10,87%),   davanone   (9.84%),   a-humulene   (7.59%),   a-curcumene (3.35%), germacrene D (3.09%), calarene (2.42%), a-muurolene (2.27%), p-cymene (1.79%), 1,8 cineole (1.59%) , δ-cadinene (1.59%), a-copaene (1.12%), nerolidol B (1%) and ß-ocimene (0.54%) were detected as major components. Antibacterial activity assay of Lantana camara L flowers essential oil was examined against four bacterial strains including Gram (+and-) by using disk diffusion agar method. All the tested Gram (+and-) bacterial strains displayed slight to moderate antibacterial activity (7 – 10 mm) against all concentrations of essential oil. The essential oil of Lantana camara L flowers showed remarkable antibacterial activity with inhibition in concentrations of 10% and 20% against the phatogen bacterias Basillus subtilis (9.6 mm; 10.8 mm), Propionibacterium acnes ( 7.6 mm; 8.7 mm), Eschechia coli ( 8.4 mm ; 9.6 mm) and Pseudomonas aeruginosa ( 8.3 mm ; 9.3 mm) showed from zone of inhibition that was formed, zone of inhibition in concentration 20% was bigger than concentration 10%.

scholarly journals Essential Oil Composition and Antimicrobial Activity of Achillea biebersteinii Afan. (Asteraceae) from Erzincan Region, Turkey

2018 ◽  
Vol 10 (3) ◽  
pp. 328-332 ◽  
Author(s):  
Emre SEVİNDİK ◽  
Sinem AYDIN ◽  
Elif EREN APAYDIN ◽  
Mustafa SÜRMEN
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Yersinia Pseudotuberculosis ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Oil Composition ◽  
Ecological Conditions ◽  
Achillea Biebersteinii

In the present study, chemical composition determination and in vitro antimicrobial effects of essential oil of Achillea biebersteinii plant grown under Erzincan ecological conditions were evaluated. Extractions were carried out with Clevenger apparatus and essential oil composition was determined by Gas Chromatography-Mass Spectrometry (GC-MS). Microorganisms used for the antimicrobial studies were Salmonella enterica serovar typhimirium ATCC 14028, Staphylococcus aureus subsp. aureus ATCC 25923, Yersinia pseudotuberculosis ATCC 911, Bacillus cereus 702 ROMA, Enterobacter aerogenes CCM 2531, Bacillus subtilis IMG 22 and Proteus vulgaris FMC 1. As a result, a total of 29 components were detected in Achillea biebersteinii.  Among them, 1,8-cineole (20.36%), cyclohexanone (8.39%), 2-cyclohexen-1-one (5.38%) and spathulenol (4.19%)  were found as the major components.  For the in vitro antimicrobial activity determination of essential oil, disc diffusion method was used in our study. Furthermore, 12-14 mm zone diameters were detected in antimicrobial activity assay. The highest resistance zone was detected against B. subtilis with 14 mm diameter while the least resistance zone was detected against Y. pseudotuberculosis, E. aerogenes and P. vulgaris with 12 mm diameter. Consequently, it was concluded that the essential oil extracted from the A. biebersteinii grown under Erzincan ecological conditions had an inhibitory effects on the pathogenic microorganisms in used method.  

INVESTIGATION INTO THE ANTIBACTERIAL ACTIVITY AND CHEMICAL COMPOSITION OF ‘CEDRUS ATLANTICA’ ESSENTIAL OIL

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (06) ◽  
pp. 51-55
Author(s):  
N Belloula ◽  
◽  
S. Dridi ◽  
A Khattaf
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Volatile Oil ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Oil Composition ◽  
Cedrus Atlantica

In the present study, Cedrus atlantica leaves volatile oil were screened for their antibacterial activity and chemical composition. The essential oil chemical constituents obtained by hydro-distillation and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The antibacterial activity was tested in vitro on Gram-negative bacteria: Escherichia coli, Pseudomonas and Klebsiella pneumonia and Gram-positive: Staphylococcus aureus, Bacillus, and Enterococcus faecalis, using disc-diffusion method. Twenty five compounds were identified in leaves oil representing 66.0.9 % of the total oil composition. Cedrus atlantica essential oil yield was 1.64% and major compounds were α-pinene (8,65%), himachalene (8.41%), β-himachalene (8,14%), σ-himachalene (6,26%), cis-α-atlantone (5,11%), himachalol (4,25%) and α-himachalene (3,25%), germacrene D (3.15%), β-caryophyllene (2.14%), cadinene (2.00%), β-pinene (1.95%), humulene (1.20%) and β-copaene (1.16%). In both strain, Cedrus atlantica oil exhibited very considerable antibacterial activity.

Sign in / Sign up

Export Citation Format

Share Document