Efficient knockout of the phytoene desaturase gene in a hybrid poplar (Populus alba × Populus glandulosa) using the CRISPR/Cas9 system with a single gRNA

2021 ◽  
Author(s):  
Eun-Kyung Bae ◽  
Hyunmo Choi ◽  
Ji Won Choi ◽  
Hyoshin Lee ◽  
Sang-Gyu Kim ◽  
...  
Keyword(s):  
Hybrid Poplar ◽  
Phytoene Desaturase ◽  
Populus Alba ◽  
Desaturase Gene

Silenced Phytoene desaturase Gene as a Scorable Marker for Plant Genetic Transformation

2014 ◽  
Vol 13 (2) ◽  
pp. 80-84
Author(s):  
Vikas Yadav Patade ◽  
Deepti Khatri ◽  
Atul Grover ◽  
Maya Kumari ◽  
Sanjay Mohan Gupta ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Phytoene Desaturase ◽  
Desaturase Gene

scholarly journals Rose Phytoene Desaturase Gene Silencing by Apple Latent Spherical Virus Vectors

HortScience ◽  
2012 ◽  
Vol 47 (9) ◽  
pp. 1278-1282 ◽  
Author(s):  
Hiroaki Ito ◽  
Masaki Ochiai ◽  
Hiroaki Kato ◽  
Katsuhiro Shiratake ◽  
Daigo Takemoto ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Gene Function ◽  
Molecular Breeding ◽  
Phytoene Desaturase ◽  
Virus Induced Gene Silencing ◽  
Desaturase Gene ◽  
Apple Latent Spherical Virus ◽  
The Rose ◽  
Highly Uniform ◽  
Secondary Shoot

We have succeeded in establishing a virus-induced gene silencing (VIGS) of rose using Apple latent spherical virus (ALSV) vectors. An ALSV infection on rose did not cause any symptoms like those observed on other plant species and grew healthy. We have cloned and sequenced the phytoene desaturase (PDS) gene in wild rose, then used its fragment for silencing the rose internal PDS gene. The silencing phenotypes such as the highly uniform photo-bleached phenotype with PDS inhibitions were observed on the upper leaves of primary shoots and on a secondary shoot of R. rugosa for more than 5 months. ALSV vectors seemed useful for analyzing gene function and for the molecular breeding of rose.

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