Estimation of dominant microbial population sizes in the anaerobic granular sludge of a full-scale UASB treating streptomycin wastewater by PCR-DGGE

The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing the humic model compound, anthraquinone-2,6-disulfonate (AQDS), as a terminal electron acceptor. Complete reduction of AQDS was achieved by the granular sludge for a prolonged period in an anaerobic bioreactor provided with a mixture of volatile fatty acids as a substrate. Phylogenetic analysis revealed the enrichment and immobilization of AQDS-respiring bacteria appearing as dominant organisms in the microbial population of the AQDS-supplemented reactor, compared to a reactor control operated under methanogenic conditions. The consistent quinone-reducing capacity observed in the consortium indicates that it is feasible to apply quinone-reducing microorganisms in continuous bioreactors and this ability can potentially be important in wastewaters rich in humic substances. The quinone reducing activity could also be applied to accelerate the conversion of xenobiotics susceptible to reductive biotransformations such as azo dyes and polychlorinated compounds in continuous bioreactors.

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Startup and performance of full-scale anaerobic granular sludge blanket reactor treating high strength inhibitory acrylic acid wastewater

Bioresource Technology ◽

10.1016/j.biortech.2020.123975 ◽

2020 ◽

Vol 317 ◽

pp. 123975

Author(s):

Kuan-Yeow Show ◽

Yue-Gen Yan ◽

Jian Zhao ◽

Jie Shen ◽

Zhong-Xu Han ◽

...

Keyword(s):

Acrylic Acid ◽

Granular Sludge ◽

High Strength ◽

Full Scale ◽

Anaerobic Granular Sludge ◽

And Performance

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Identical full-scale biogas-lift reactors (BLRs) with anaerobic granular sludge and residual activated sludge for brewery wastewater treatment and kinetic modeling

Journal of Environmental Sciences ◽

10.1016/s1001-0742(12)60268-x ◽

2013 ◽

Vol 25 (10) ◽

pp. 2031-2040 ◽

Cited By ~ 12

Author(s):

Fu Xu ◽

Zhenxing Huang ◽

Hengfeng Miao ◽

Hongyan Ren ◽

Mingxing Zhao ◽

...

Keyword(s):

Wastewater Treatment ◽

Activated Sludge ◽

Kinetic Modeling ◽

Granular Sludge ◽

Full Scale ◽

Anaerobic Granular Sludge ◽

Brewery Wastewater

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Toxicity of Sulfite and Cadmium to Anaerobic Granular Sludge

Water Quality Research Journal ◽

10.2166/wqrj.1994.037 ◽

1994 ◽

Vol 29 (4) ◽

pp. 581-598

Author(s):

C.F. Shew ◽

N. Kosaric

Keyword(s):

Granular Sludge ◽

Upflow Anaerobic Sludge Blanket ◽

Shock Load ◽

Anaerobic Sludge ◽

Anaerobic Granular Sludge ◽

Scanning Electron Micrographs ◽

Bacterial Growth Rate ◽

Digestion Rate ◽

Sulfate Reducing ◽

Load Conditions

Abstract Toxicity of sulfite (Na2SO3) and cadmium (CdCl2) ions to anaerobic granular sludge was investigated in 1.2 litre bench-scale upflow anaerobic sludge blanket (UASB) reactors during process acclimation and shock load conditions. Minimal sulfite toxicity was observed under gradual and shock load conditions at sulfite concentrations of up to 1000 mg S/L if proper acclimation was allowed to occur. No long-term toxic effects were observed although the COD digestion rate was temporarily inhibited by shock load of sulfite. Scanning electron micrographs indicated that more sulfate-reducing bacteria were present in the granules developed in the reactors with sulfite supplement although rod-shaped Methanosaeta-like bacteria were still dominant. High bacterial growth rate was observed in the reactors which were supplied with the feed containing sulfite. The COD digestion rate was inhibited at a cadmium loading rate of 2.4 g Cd per day under both acclimation and shock load conditions. Acclimation did not seem to improve the bacteria to tolerate the toxicity of cadmium. The concentration of free cadmium was very low in the reactors under normal conditions, but increased rapidly when the COD digestion in the reactors ceased. The bacteria could not be reactivated after inhibited by cadmium. When reactors were operated at low specific COD loading rates, more inorganic precipitates were formed inside the granules which consequently settled faster.

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