Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge

2003 ◽  
Vol 48 (6) ◽  
pp. 9-16 ◽  
Author(s):  
F.J. Cervantes ◽  
T. Duong-Dac ◽  
K. Roest ◽  
A.D.L. Akkermans ◽  
G. Lettinga ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Model Compound ◽  
Microbial Population ◽  
Basal Medium ◽  
Granular Sludge ◽  
Anaerobic Granular Sludge ◽  
Terminal Electron Acceptor ◽  
Reducing Activity ◽  
Reducing Capacity

The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing the humic model compound, anthraquinone-2,6-disulfonate (AQDS), as a terminal electron acceptor. Complete reduction of AQDS was achieved by the granular sludge for a prolonged period in an anaerobic bioreactor provided with a mixture of volatile fatty acids as a substrate. Phylogenetic analysis revealed the enrichment and immobilization of AQDS-respiring bacteria appearing as dominant organisms in the microbial population of the AQDS-supplemented reactor, compared to a reactor control operated under methanogenic conditions. The consistent quinone-reducing capacity observed in the consortium indicates that it is feasible to apply quinone-reducing microorganisms in continuous bioreactors and this ability can potentially be important in wastewaters rich in humic substances. The quinone reducing activity could also be applied to accelerate the conversion of xenobiotics susceptible to reductive biotransformations such as azo dyes and polychlorinated compounds in continuous bioreactors.

Hybrid reactor performance in pentachlorophenol (pcp) removal by anaerobic granules

2001 ◽  
Vol 44 (4) ◽  
pp. 137-144 ◽  
Author(s):  
M. De Almeida Prado Montenegro ◽  
E. De Mattos Moraes ◽  
H. Moreira Soares ◽  
R. Filomena Vazoller
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Culture Media ◽  
Biogas Production ◽  
Removal Rate ◽  
Oxygen Demand ◽  
Granular Sludge ◽  
Concentration Addition ◽  
Hybrid Reactor ◽  
Reactor Performance

The present research aimed at evaluating pentachlorophenol (PCP) degradation in a hybrid reactor supplied with a mixture of fatty acids (propionic, butyric, acetic and lactic) and methanol. The performance of the reactor is remarkably stable and efficient during PCP additions at range of 2.0 to 21.0 mg/L. The reduction of chemical oxygen demand (COD) was around 97% and methane was found to be 86% in the biogas production. The efficiency of volatile fatty acids breakdown was 93%, 64% and 74% respectively for butyric, propionic and acetic. PCP total removal of more than 99% was reached by granular sludge activities formed during 21 months of reactor operation. Methanogenic microorganisms predominance was noticed with 105 to 106 cells/mL during enumeration on methanol or lactate added to sulfate culture media. The removal rate was 1.07 mg PCP · g−1 VS · d−1 during the highest PCP concentration addition.

Optimalisasi Penggunaan Level Sabun Kalsium Minyak Kedelai dalam Ransum Terhadap Karakteristik Fermentasi, Populasi Mikroba dan Kecernaan Nutrien Secara In Vitro Menggunakan Cairan Rumen Sapi Bali

2018 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Ali Bain ◽  
Komang G. Wiryawan ◽  
Dewi Apri Astuti ◽  
Chairrusyuhur Arman ◽  
Sri Suharti
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Microbial Population ◽  
Total Volatile ◽  
Calcium Soap ◽  
Total Bacteria ◽  
Range Test

ABSTRAKPenelitian ini bertujuan untuk mengevaluasi optimalisasi penggunaan level sabun kalsium yang berbeda dalam ransum secara in vitro terhadap karakteristik fermentasi, populasi mikroba dan kecernaan nutrien ransum, menggunakan sumber inokulum cairan rumen sapi Bali. Penelitian dilaksanakan menggunakan rancangan acak kelompok dengan 4 jenis perlakuan dalam 3 ulangan.  Ransum penelitian terdiri atas : R1, 40% rumput lapang (RL) + 60% konsentrat (K), R2 (40% RL + 60% K, mengandungn 2.5% SCa-kedelai), R3 (40%  RL + 60% K, mengandung 5% SCa-kedelai), R4 (40%  RL + 60% K, mengandung 7.5% SCa-kedelai). Variabel yang diukur terdiri atas karakteristik fermentasi in vitro (pH, N-NH3, produksi total volatile fatty acids), populasi mikroorganisme (total bakteri dan total protozoa), dan kecernaan nutrien (kecernaan bahan kering dan kecernaan bahan organik). Data dianalisis menggunakan analysis of varians  (ANOVA) dan perbedaan antara perlakuan diuji dengan Duncan’s Multiple Range Test.  Hasil penelitian menunjukkan, penambahan SCa-kedelai pada level yang berbeda dalam konsentrat tidak mempengaruhi pH, konsentrasi amonia, populasi mikroba, kecernaan bahan kering dan kecernaan bahan organik fermentasi tetapi berpengaruh sangat nyata (P<0.002) terhadap produksi total VFA. Produksi total VFA tertinggi diperoleh pada ransum perlakuan R4 (konsentrat yang mengandung 7.5% SCa-kedelai). Produksi total VFA tertinggi diperoleh pada perlakuan R4 dan produksi total VFA paling rendah terjadi pada perlakuan ransum R1 (kontrol).  Produksi total VFA  ransum R1 dan R2 dan ransum R2 dan R3 tidak berbeda nyata namun produksi total VFA ransum R3 lebih tinggi dibanding ransum kontrol. Penambahan SCa-kedelai dalam konsentrat menghasilkan produksi total VFA tetinggi namun mulai menekan populasi total bakteri. Mempertimbangkan hasil peubah fermentasi dan populasi mikroba serta biaya pembuatan produk sabun kalsium, SCa-kedelai pada level 5% merupakan level penggunaan SCa-kedelai yang terbaik dalam ransum.Kata kunci: sabun kalsium, fermentasi, in vitro, kecernaanABSTRACTThe study to evaluate the optimalization the different level of soybean oil calcium soap (CaS-soybean) in ration on in vitro fermentation using Bali cattle rumen fluid. The experiment was arranged in a complete randomized block design with 4 different ration treatments and 3 replicates. Ration treatments were R1: 40% native grass (NG) + 60% concentrate (C), R2 : 40% NG + 60% C, supplemented with 2.5% soybean oil calcium soap (CaS-soybean), and R3 (40% NG + 60% C, supplemented with 5.0% CaS-soybean and R4 (40% NG + 60% C, supplemented with 7.5% CaS-soybean). Variables measured were fermentation characteristics (pH, NH3-N, total volatile fatty acids), microbial population (total bacteria and total protozoa), and nutrient digestibility (dry matter and organic matter digestibility). Data were analyzed using analysis of variance (ANOVA) and the differences between treatments means were examined by Duncan Multiple Range Test. Results of the study  showed that the four different level of CaS-soybean in ration did not have any significant effect (P>0,05) on pH, NH3-N, total bacteria, total protozoa, dry matter and organic matter digestibility. The treatments significantly increased (P<0.05) the production of total volatile fatty acids.  The highest production of total VFA was obtained from ration R4 (concentrate containing 7.5% CaS-soybean) and the lowest was obtained from treatment R1 (control). There were no significant differences between R1 and  R2, and between R2 and R3 on the total VFA production. However, the total productions of VFA in ration R3 were higher than that of the control ration. The addition of CaS-soybean in the concentrate had increased the total production of VFA, but at the same time, it began pressing the total population of bacteria. Considering the results of the fermentation variables and microbial population as well as the cost of making calcium soap products, CaS-soybean at 5% level was selected as the best level of CaS-soybean in ration.Keywords : calcium soap, fermentation, in vitro, digestibility

scholarly journals Influence of adsorption and anaerobic granular sludge characteristics on long chain fatty acids inhibition process

2012 ◽  
Vol 46 (16) ◽  
pp. 5268-5278 ◽  
Author(s):  
J. Palatsi ◽  
R. Affes ◽  
B. Fernandez ◽  
M.A. Pereira ◽  
M.M. Alves ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Granular Sludge ◽  
Anaerobic Granular Sludge ◽  
Long Chain Fatty Acids ◽  
Sludge Characteristics ◽  
Inhibition Process ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Psychrophilic anaerobic treatment of low strength wastewaters

1999 ◽  
Vol 39 (5) ◽  
pp. 203-210 ◽  
Author(s):  
Salih Rebac ◽  
Jules B. van Lier ◽  
Piet Lens ◽  
Alfons J. M. Stams ◽  
Freddy Dekkers ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Oxygen Demand ◽  
Granular Sludge ◽  
Anaerobic Treatment ◽  
Cod Removal ◽  
High Rate ◽  
Organic Loading Rate ◽  
Substrate Conversion ◽  
Low Strength

Psychrophilic (2 to 20°C) anaerobic treatment of low strength synthetic and malting wastewater was investigated using a single and two module expanded granular sludge bed (EGSB) reactor system. The chemical oxygen demand (COD) removal efficiencies found in the experiments exceeded 90 % in the single module reactor at an organic loading rate up to 12 g COD dm−3 day−1 and a HRT of 1.6 h at 10-12°C ambient temperature using influent concentrations ranging from 500 to 800 mg COD dm−3. When a two module EGSB system was used at the temperature range 10-15°C, soluble COD removal and volatile fatty acids removal of 67-78% and 90-96% were achieved, respectively, and an OLR between 2.8-12.3 kg COD m−3 day−1 and a HRT of 3.5 h. The second module serves mainly as a scavenger of non-degraded volatile fatty acids (VFA) from the first module. The optimal temperatures for substrate conversion of reactor sludge, after it has been exposed to long term psychrophilic conditions, were similar to those of the original mesophilic inoculum. The specific activities of the sludge in the reactor increased in time by a factor 3, indicating enrichment of methanogens and acetogens even at low temperatures. By adapting the process design to the expected prevailing conditions inside the reactor, the loading potentials and overall stability of the anaerobic high-rate process may be distinctly improved under psychrophilic conditions. The results obtained clearly reveal the big potentials of anaerobic wastewater treatment under low ambient (10-12°C) temperature conditions for low strength wastewaters, very likely including domestic sewage.

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