The production of laccases by white-rot fungi under solid-state fermentation conditions

White rot fungi of Marasmius sp. is a fungus which produce laccase in high activity. Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production. Laccase was produced in flask by batch process using Solid State Fermentation (SSF). The optimisation was conducted by statistically of full factorial design. The particle size, moisture content, and Cu concentration were investigated in this study. Rice straw was used as solid substrate and the glycerol was used as the carbon sources in modified Kirk medium. The results showed that particle size of rice straw did not affect significantly to the enzyme activity. The highest laccase activity of 4.45 IU/g dry weight was obtained at the moisture content of 61% and Cu concentration of 0.1 mM.Keywords: laccase, Marasmius sp., optimisation, rice straw, solid state fermentation ABSTRAKJamur pelapuk putih, Marasmius sp. merupakan jamur yang menghasilkan enzim lakase dengan aktivitas tinggi. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin. Kemampuan ini dapat digunakan untuk proses pengolahan awal bahan lignoselulosa pada pembuatan bioetanol. Produksi lakase dilakukan dalam labu dengan modus batch menggunakan fermentasi kultur padat. Optimisasi produksi enzim lakase dengan metode fermentasi padat dilakukan dengan rancangan percobaan faktorial penuh. Pengaruh ukuran partikel, kelembapan, dan konsentrasi Cu diuji dengan medium penyangga jerami dengan menambahkan gliserol dalam medium Kirk termodifikasi sebagai sumber karbon. Penelitian ini menunjukkan bahwa ukuran jerami tidak berpengaruh signifikan terhadap aktivitas enzim. Aktivitas enzim lakase maksimum terjadi pada saat kelembapan 61% dan konsentrasi Cu 0,1 mM dengan aktivitas enzim lakase/berat kering tertinggi mencapai 4,45 IU/g.Kata kunci: lakase, Marasmius sp., optimisasi, jerami, fermentasi kultur padat

Download Full-text

Correlation of brightening with cumulative enzyme activity related to lignin biodegradation during biobleaching of kraft pulp by white rot fungi in the solid-state fermentation system.

Applied and Environmental Microbiology ◽

10.1128/aem.61.2.617-622.1995 ◽

1995 ◽

Vol 61 (2) ◽

pp. 617-622 ◽

Cited By ~ 58

Author(s):

N Katagiri ◽

Y Tsutsumi ◽

T Nishida

Keyword(s):

Enzyme Activity ◽

Solid State ◽

Solid State Fermentation ◽

Kraft Pulp ◽

White Rot Fungi ◽

White Rot ◽

Lignin Biodegradation ◽

Fermentation System

Download Full-text

White rot fungi pretreatment to advance volatile fatty acid production from solid-state fermentation of solid digestate: Efficiency and mechanisms

Energy ◽

10.1016/j.energy.2018.08.082 ◽

2018 ◽

Vol 162 ◽

pp. 534-541 ◽

Cited By ~ 23

Author(s):

Wei Fang ◽

Panyue Zhang ◽

Xuedong Zhang ◽

Xuefeng Zhu ◽

Jules B. van Lier ◽

...

Keyword(s):

Fatty Acid ◽

Solid State ◽

Solid State Fermentation ◽

Acid Production ◽

Volatile Fatty Acid ◽

White Rot Fungi ◽

White Rot ◽

Fatty Acid Production

Download Full-text

Lignocellulolytic activity of Pleurotus ostreatus under solid state fermentation using silage, stover, and cobs of maize

BioResources ◽

10.15376/biores.16.2.3797-3807 ◽

2021 ◽

Vol 16 (2) ◽

pp. 3797-3807

Author(s):

Magdah Ganash ◽

Tarek M. Abdel Ghany ◽

Mohamed A. Al Abboud ◽

Mohamed M. Alawlaqi ◽

Husam Qanash ◽

...

Keyword(s):

Solid State ◽

Incubation Period ◽

Solid State Fermentation ◽

Pleurotus Ostreatus ◽

White Rot Fungi ◽

Value Added ◽

Extracellular Protein ◽

White Rot ◽

White Rot Fungus ◽

Lignocellulosic Substrate

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.

Download Full-text