Response of soil bacterial communities to lead and zinc pollution revealed by Illumina MiSeq sequencing investigation

Salinized land in the China’s Xinjiang Region is being reclaimed for continuous cotton production. The specific objectives of this field study were (i) to compare bacterial composition and diversity in unfarmed (i.e., unreclaimed) and continuously (5, 10, 15, and 20 years) cropped soils and (ii) to explore correlations between soil properties and the bacterial communities identified by Illumina MiSeq sequencing. The results showed that bacterial species richness and diversity increased for 10–15 years and then declined when salinized land was reclaimed for cotton production. Proteobacteria and Firmicutes were the dominant phyla in unfarmed soil. Continuous cropping reduced the abundance of Firmicutes but increased that of Chloroflexi, Acidobacteria, and Actinobacteria. Cluster analyses showed that the greatest similarities in bacterial communities were between the 5- and 10-year treatments and between the 15- and 20-year treatments. Soil pH, electrical conductivity, alkali-hydrolyzable N, and available P were significantly correlated with bacterial community distribution. Overall, cotton production improved soil physicochemical properties and altered the structure and composition of soil bacterial communities compared with unfarmed soil. These positive effects began to decrease after 10–15 years of continuous cotton production.

Download Full-text

Airborne Bacterial Communities of PM2.5 in Beijing-Tianjin-Hebei Megalopolis, China as Revealed by Illumina MiSeq Sequencing: A Case Study

Aerosol and Air Quality Research ◽

10.4209/aaqr.2016.02.0087 ◽

2017 ◽

Vol 17 (3) ◽

pp. 788-798 ◽

Cited By ~ 26

Author(s):

Jing-Feng Gao ◽

Xiao-Yan Fan ◽

Hong-Yu Li ◽

Kai-Ling Pan

Keyword(s):

Bacterial Communities ◽

Illumina Miseq ◽

Illumina Miseq Sequencing ◽

Miseq Sequencing

Download Full-text

High Diversity of Bacterial Communities in Developmental Stages of Bactrocera carambolae (Insecta: Tephritidae) Revealed by Illumina MiSeq Sequencing of 16S rRNA Gene

Current Microbiology ◽

10.1007/s00284-017-1287-x ◽

2017 ◽

Vol 74 (9) ◽

pp. 1076-1082 ◽

Cited By ~ 20

Author(s):

Hoi-Sen Yong ◽

Sze-Looi Song ◽

Kah-Ooi Chua ◽

Phaik-Eem Lim

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Bacterial Communities ◽

Developmental Stages ◽

Illumina Miseq ◽

Rrna Gene ◽

Illumina Miseq Sequencing ◽

Miseq Sequencing ◽

Bactrocera Carambolae ◽

High Diversity

Download Full-text

Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon

Plant Soil and Environment ◽

10.17221/419/2021-pse ◽

2021 ◽

Vol 67 (No. 12) ◽

pp. 721-728

Author(s):

Jibo Shi ◽

Xiaoya Gong ◽

Muhammad Khashi u Rahman ◽

Qing Tian ◽

Xingang Zhou ◽

...

Keyword(s):

Root Exudates ◽

Bacterial Communities ◽

Plant Root ◽

Microbial Community Composition ◽

Illumina Miseq ◽

Wheat Root ◽

Illumina Miseq Sequencing ◽

Miseq Sequencing ◽

Streptomyces Spp ◽

Total Bacteria

In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing. The qPCR results showed that wheat root exudates significantly increased the abundance of total bacteria, Pseudomonas, Bacillus and Streptomyces spp. Illumina MiSeq sequencing results showed that wheat root exudates significantly changed the bacterial community structure and composition. These results indicated that plant root exudates play a role in plant-to-plant signalling, strongly affect the microbial community composition.

Download Full-text

Assessing the spatial and temporal variability of bacterial communities in two Bardenpho wastewater treatment systems via Illumina MiSeq sequencing

The Science of The Total Environment ◽

10.1016/j.scitotenv.2018.12.141 ◽

2019 ◽

Vol 657 ◽

pp. 1543-1552 ◽

Cited By ~ 17

Author(s):

Jia Xue ◽

Bradley W. Schmitz ◽

Kevin Caton ◽

Bowen Zhang ◽

Jovanny Zabaleta ◽

...

Keyword(s):

Wastewater Treatment ◽

Temporal Variability ◽

Bacterial Communities ◽

Illumina Miseq ◽

Spatial And Temporal Variability ◽

Illumina Miseq Sequencing ◽

Miseq Sequencing ◽

Wastewater Treatment Systems

Download Full-text

Effects of temperature onParamoeba peruransgrowth in culture and the associated microbial community

Parasitology ◽

10.1017/s0031182018001798 ◽

2018 ◽

Vol 146 (4) ◽

pp. 533-542 ◽

Cited By ~ 2

Author(s):

O. Benedicenti ◽

C. J. Secombes ◽

C. Collins

Keyword(s):

Microbial Community ◽

Bacterial Communities ◽

Bacterial Community Composition ◽

Growth Dynamics ◽

Illumina Miseq ◽

Illumina Miseq Sequencing ◽

Miseq Sequencing ◽

Effects Of Temperature ◽

Over Time

AbstractPopulation growth,in vitro, of threeParamoeba peruranscultures, one polyclonal (G) and two clonal (B8, CE6, derived from G), previously shown to differ in virulence (B8 > G > CE6), was compared at 10 and 15 °C. B8 showed a significantly higher increase in attached and in suspended amoebae over time at 15 and 10 °C, respectively. CE6 and G also had significantly higher numbers of suspended amoebae at 10 °C compared with 15 °C at experiment termination. However, in contrast to B8, numbers of attached amoebae were significantly higher at 10 °C in CE6 but showed a similar trend in G at the end of the experiment. Numbers of both suspended and attached amoebae were lower in B8 compared with CE6 and G. Significant differences in bacterial community composition and/or relative abundances were found, between cultures, between temperatures and between the same culture with and without amoebae, based on 16S rRNA Illumina MiSeq sequencing. Bacterial diversity was lower in B8 and CE6 compared with G, possibly reflecting selection during clonal isolation. The results indicate that polyclonalP. peruranspopulations may contain amoebae displaying different growth dynamics. Further studies are required to determine if these differences are linked to differences seen in the bacterial communities.

Download Full-text