The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum

2008 ◽  
Vol 51 (4) ◽  
pp. 353-361 ◽  
Author(s):  
ChengYun Yang ◽  
YouGuo Li ◽  
Li Wei ◽  
GuoJun Cheng ◽  
JunChu Zhou
Keyword(s):  
Rhizobium Leguminosarum ◽  
Symbiotic Interaction ◽  
Indigenous Plasmids

scholarly journals Genetic and Metabolic Divergence within a Rhizobium leguminosarum bv. trifolii Population Recovered from Clover Nodules

2010 ◽  
Vol 76 (14) ◽  
pp. 4593-4600 ◽  
Author(s):  
Jerzy Wielbo ◽  
Monika Marek-Kozaczuk ◽  
Andrzej Mazur ◽  
Agnieszka Kubik-Komar ◽  
Anna Skorupska
Keyword(s):  
Population Genetic ◽  
Rhizobium Leguminosarum ◽  
Local Population ◽  
Test Results ◽  
Adaptive Potential ◽  
Symbiotic Interaction ◽  
Large Size ◽  
Metabolic Versatility ◽  
Physiological Properties ◽  
Nodule Population

ABSTRACT Rhizobia are able to establish symbiosis with leguminous plants and usually occupy highly complex soil habitats. The large size and complexity of their genomes are considered advantageous, possibly enhancing their metabolic and adaptive potential and, in consequence, their competitiveness. A population of Rhizobium leguminosarum bv. trifolii organisms recovered from nodules of several clover plants growing in each other's vicinity in the soil was examined regarding possible relationships between their metabolic-physiological properties and their prevalence in such a local population. Genetic and metabolic variability within the R. leguminosarum bv. trifolii strains occupying nodules of several plants was of special interest, and both types were found to be considerable. Moreover, a prevalence of metabolically versatile strains, i.e., those not specializing in utilization of any group of substrates, was observed by combining statistical analyses of Biolog test results with the frequency of occurrence of genetically distinct strains. Metabolic versatility with regard to nutritional requirements was not directly advantageous for effectiveness in the symbiotic interaction with clover: rhizobia with specialized metabolism were more effective in symbiosis but rarely occurred in the population. The significance of genetic and, especially, metabolic complexity of bacteria constituting a nodule population is discussed in the context of strategies employed by bacteria in competition.

scholarly journals Mutation in the pssZ Gene Negatively Impacts Exopolysaccharide Synthesis, Surface Properties, and Symbiosis of Rhizobium leguminosarum bv. trifolii with Clover

Genes ◽  
2018 ◽  
Vol 9 (7) ◽  
pp. 369 ◽  
Author(s):  
Paulina Lipa ◽  
José-María Vinardell ◽  
Joanna Kopcińska ◽  
Agnieszka Zdybicka-Barabas ◽  
Monika Janczarek
Keyword(s):  
Host Plant ◽  
Surface Properties ◽  
Essential Role ◽  
Rhizobium Leguminosarum ◽  
Symbiotic Interaction ◽  
High Identity ◽  
Tn5 Transposon ◽  
Transposon Insertion ◽  
Altered Cell ◽  
Enzymatic Complex

Rhizobium leguminosarum bv. trifolii is a soil bacterium capable of establishing a nitrogen-fixing symbiosis with clover plants (Trifolium spp.). This bacterium secretes large amounts of acidic exopolysaccharide (EPS), which plays an essential role in the symbiotic interaction with the host plant. This polymer is biosynthesized by a multi-enzymatic complex located in the bacterial inner membrane, whose components are encoded by a large chromosomal gene cluster, called Pss-I. In this study, we characterize R. leguminosarum bv. trifolii strain Rt297 that harbors a Tn5 transposon insertion located in the pssZ gene from the Pss-I region. This gene codes for a protein that shares high identity with bacterial serine/threonine protein phosphatases. We demonstrated that the pssZ mutation causes pleiotropic effects in rhizobial cells. Strain Rt297 exhibited several physiological and symbiotic defects, such as lack of EPS production, reduced growth kinetics and motility, altered cell-surface properties, and failure to infect the host plant. These data indicate that the protein encoded by the pssZ gene is indispensable for EPS synthesis, but also required for proper functioning of R. leguminosarum bv. trifolii cells.

scholarly journals Towards understanding Afghanistan pea symbiotic phenotype through the molecular modeling of the interaction between LykX-Sym10 receptor heterodimer and Nod factors

2020 ◽  
Author(s):  
Ya.V. Solovev ◽  
A.A. Igolkina ◽  
P.O. Kuliaev ◽  
A.S. Sulima ◽  
V.A. Zhukov ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Rhizobium Leguminosarum ◽  
Genetic Determinants ◽  
Amino Acid Polymorphism ◽  
Symbiotic Interaction ◽  
Modeling Methodology ◽  
European Variant ◽  
Symbiotic Phenotype ◽  
The Difference ◽  
The Stability

AbstractThe difference in symbiotic specificity between peas of Afghanistan and European phenotypes was interrogated using molecular modeling. Considering segregating amino acid polymorphism, we examined interactions of pea LykX-Sym10 receptor heterodimers with four forms of Nod factor (NF) that varied in natural decorations (acetylation and length of the glucosamine chain). First, we showed the stability of the LykX-Sym10 dimer during molecular dynamics (MD) in solvent and in the presence of a membrane. Then, four NFs were separately docked to one European and two Afghanistan dimers, and the results of these interactions were in line with corresponding pea symbiotic phenotypes. The European variant of the LykX-Sym10 dimer effectively interacts with both acetylated and non-acetylated forms of NF, while the Afghanistan variants successfully interact with the acetylated form only. We additionally demonstrated that the length of the NF glucosamine chain contributes to controlling the effectiveness of the symbiotic interaction. The obtained results support a recent hypothesis that the LykX gene is a suitable candidate for the unidentified Sym2 allele, the determinant of pea specificity towards Rhizobium leguminosarum bv. viciae strains producing NFs with or without an acetylation decoration. The developed modeling methodology demonstrated its power in multiple searches for genetic determinants, when experimental detection of such determinants has proven extremely difficult.

The Vicia sativa spp. nigra - Rhizobium leguminosarum bv. viciae symbiotic interaction is improved by Azospirillum brasilense

2011 ◽  
Vol 356 (1-2) ◽  
pp. 165-174 ◽  
Author(s):  
Lara Star ◽  
Ofra Matan ◽  
Marta S. Dardanelli ◽  
Yoram Kapulnik ◽  
Saul Burdman ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Rhizobium Leguminosarum ◽  
Vicia Sativa ◽  
Symbiotic Interaction

scholarly journals Genetic Variation in Host-Specific Competitiveness of the Symbiont Rhizobium leguminosarum Symbiovar viciae

2021 ◽  
Vol 12 ◽  
Author(s):  
Stéphane Boivin ◽  
Frederic Mahé ◽  
Frédéric Debellé ◽  
Marjorie Pervent ◽  
Mathilde Tancelin ◽  
...  
Keyword(s):  
Core Collection ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Dna Barcode ◽  
Partner Choice ◽  
Comparative Genomic ◽  
Emergent Properties ◽  
Symbiotic Interaction ◽  
Dna Metabarcoding ◽  
Nodulated Root

Legumes of the Fabeae tribe form nitrogen-fixing root nodules resulting from symbiotic interaction with the soil bacteria Rhizobium leguminosarum symbiovar viciae (Rlv). These bacteria are all potential symbionts of the Fabeae hosts but display variable partner choice when co-inoculated in mixture. Because partner choice and symbiotic nitrogen fixation mostly behave as genetically independent traits, the efficiency of symbiosis is often suboptimal when Fabeae legumes are exposed to natural Rlv populations present in soil. A core collection of 32 Rlv bacteria was constituted based on the genomic comparison of a collection of 121 genome sequences, representative of known worldwide diversity of Rlv. A variable part of the nodD gene sequence was used as a DNA barcode to discriminate and quantify each of the 32 bacteria in mixture. This core collection was co-inoculated on a panel of nine genetically diverse Pisum sativum, Vicia faba, and Lens culinaris genotypes. We estimated the relative Early Partner Choice (EPC) of the bacteria with the Fabeae hosts by DNA metabarcoding on the nodulated root systems. Comparative genomic analyses within the bacterial core collection identified molecular markers associated with host-dependent symbiotic partner choice. The results revealed emergent properties of rhizobial populations. They pave the way to identify genes related to important symbiotic traits operating at this level.

scholarly journals Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
E. Menéndez ◽  
M. Robledo ◽  
J. I. Jiménez-Zurdo ◽  
E. Velázquez ◽  
R. Rivas ◽  
...  
Keyword(s):  
Root Hair ◽  
Cell Walls ◽  
Rhizobium Leguminosarum ◽  
Plant Responses ◽  
Symbiotic Interaction ◽  
Growing Cell ◽  
Ensifer Meliloti ◽  
Rhizobial Species ◽  
Related Plant

Abstract Primary infection of legumes by rhizobia involves the controlled localized enzymatic breakdown of cell walls at root hair tips. Previous studies determined the role of rhizobial CelC2 cellulase in different steps of the symbiotic interaction Rhizobium leguminosarum-Trifolium repens. Recent findings also showed that CelC2 influences early signalling events in the Ensifer meliloti-Medicago truncatula interaction. Here, we have monitored the root hair phenotypes of two legume plants, T. repens and M. sativa, upon inoculation with strains of their cognate and non-cognate rhizobial species, R. leguminosarum bv trifolii and E. meliloti, (over)expressing the CelC2 coding gene, celC. Regardless of the host, CelC2 specifically elicited ‘hole-on-the-tip’ events (Hot phenotype) in the root hair apex, consistent with the role of this endoglucanase in eroding the noncrystalline cellulose found in polarly growing cell walls. Overproduction of CelC2 also increased root hair tip redirections (RaT phenotype) events in both cognate and non-cognate hosts. Interestingly, heterologous celC expression also induced non-canonical alterations in ROS (Reactive Oxygen Species) homeostasis at root hair tips of Trifolium and Medicago. These results suggest the concurrence of shared unspecific and host-related plant responses to CelC2 during early steps of symbiotic rhizobial infection. Our data thus identify CelC2 cellulase as an important determinant of events underlying early infection of the legume host by rhizobia.

scholarly journals Specificity of the symbiotic interaction of bacteria of the genus Rhizobium leguminosarum bv. viciae with plants of the tribe Vicieae

2018 ◽  
Vol 16 (4) ◽  
pp. 51-60
Author(s):  
Sofya A. Khapchaeva ◽  
Svetlana V. Didovich ◽  
Alexey F. Topunov ◽  
Andrey L. Mulyukin ◽  
Vasiliy S. Zotov
Keyword(s):  
Rhizobium Leguminosarum ◽  
Rpob Gene ◽  
Symbiotic Interaction ◽  
Rhizobium Strains ◽  
Industrial Strains ◽  
Nodulation Competitiveness ◽  
Total Dna ◽  
Lathyrus Sativus L ◽  
Microbial Systems ◽  
Selection Of

The estimation of nodulation competitiveness of industrial strains against the native nodule bacteria and also the analyses of distribution of strain’s genotypes which formed nodules on roots of 4 plant species was the purpose of this work. The objects of the research were rhizobium strains which formed nodules on roots of plant (the nodule-forming units – NFU), obtained in field experiment with application of preseeding processing of seeds of pea (Pisum satіvum L.), fava beans (Vicia faba L.), lathyrus (Lathyrus sativus L.) and lentil (Lens culinaris L.). The mixture of the collection strains allocated from nodules of peas and beans, and having various combinations of chromosomal and symbiotic genotypes was used for inoculation of seeds. Identification of NFU was carried out with the use of the analysis of the emitted nodule total DNA on several chromosomal markers: the rpoB gene and the hin-region, and the plasmid marker – nodD gene. It is established that only about 50% of nodules were formed by the strains used at inoculation of seeds. Besides, the combinations of chromosomal and symbiotic genotypes specific for a rhizobium – symbionts of concrete host plants have been established: IA genotype with sym-2 for P. sativum; IB genotype with sym-4 for V. faba. The results of this study create prerequisites for selection of couples: macro- and microsymbiont for the purpose of increasing efficiency of plant-microbial systems, in which the nature of symbiotic interaction defines efficiency of partners.

scholarly journals Towards Understanding Afghanistan Pea Symbiotic Phenotype Through the Molecular Modeling of the Interaction Between LykX-Sym10 Receptor Heterodimer and Nod Factors

2021 ◽  
Vol 12 ◽  
Author(s):  
Yaroslav V. Solovev ◽  
Anna A. Igolkina ◽  
Pavel O. Kuliaev ◽  
Anton S. Sulima ◽  
Vladimir A. Zhukov ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Rhizobium Leguminosarum ◽  
Genetic Determinants ◽  
Amino Acid Polymorphism ◽  
Symbiotic Interaction ◽  
Modeling Methodology ◽  
European Variant ◽  
Symbiotic Phenotype ◽  
The Difference ◽  
The Stability

The difference in symbiotic specificity between peas of Afghanistan and European phenotypes was investigated using molecular modeling. Considering segregating amino acid polymorphism, we examined interactions of pea LykX-Sym10 receptor heterodimers with four forms of Nodulation factor (NF) that varied in natural decorations (acetylation and length of the glucosamine chain). First, we showed the stability of the LykX-Sym10 dimer during molecular dynamics (MD) in solvent and in the presence of a membrane. Then, four NFs were separately docked to one European and two Afghanistan dimers, and the results of these interactions were in line with corresponding pea symbiotic phenotypes. The European variant of the LykX-Sym10 dimer effectively interacts with both acetylated and non-acetylated forms of NF, while the Afghanistan variants successfully interact with the acetylated form only. We additionally demonstrated that the length of the NF glucosamine chain contributes to controlling the effectiveness of the symbiotic interaction. The obtained results support a recent hypothesis that the LykX gene is a suitable candidate for the unidentified Sym2 allele, the determinant of pea specificity toward Rhizobium leguminosarum bv. viciae strains producing NFs with or without an acetylation decoration. The developed modeling methodology demonstrated its power in multiple searches for genetic determinants, when experimental detection of such determinants has proven extremely difficult.

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