Acid stress response in environmental and clinical strains of enteric bacteria

2012 ◽  
Vol 7 (6) ◽  
pp. 495-505 ◽  
Author(s):  
Gabriel J. Swenson ◽  
J. Stochastic ◽  
Franklyn F. Bolander ◽  
Richard A. Long
Keyword(s):  
Stress Response ◽  
Acid Stress ◽  
Enteric Bacteria ◽  
Clinical Strains ◽  
Acid Stress Response

Acid stress response in enteropathogenic gammaproteobacteria: an aptitude for survivalThis paper is one of a selection of papers published in this special issue entitled “Canadian Society of Biochemistry, Molecular & Cellular Biology 52nd Annual Meeting — Protein Folding: Principles and Diseases” and has undergone the Journal's usual peer review process.

2010 ◽  
Vol 88 (2) ◽  
pp. 301-314 ◽  
Author(s):  
Boyu Zhao ◽  
Walid A. Houry
Keyword(s):  
Stress Response ◽  
Peer Review Process ◽  
Shigella Flexneri ◽  
Acid Stress ◽  
Acid Resistance ◽  
Enteric Bacteria ◽  
Vibrio Cholera ◽  
Response Systems ◽  
Acid Stress Response ◽  
Fine Tune

Enteric bacteria such as Escherichia coli have acquired a wide array of acid stress response systems to counteract the extreme acidity encountered when invading the host’s digestive or urinary tracts. These acid stress response systems are both enzyme and chaperone based. The 3 main enzyme-based acid resistance pathways are glutamate-, arginine-, and lysine-decarboxylase pathways. They are under a complex regulatory network allowing the bacteria to fine tune its response to the external environment. HdeA and HdeB are the main chaperones involved in acid stress response. The decarboxylase systems are also found in Vibrio cholera, Vibrio vulnifus, Shigella flexneri, and Salmonella typhimurium, although some differences exist in their functional mechanism and regulation.

Integrative multiomics analysis of the acid stress response of Oenococcus oeni mutants at different growth stages

2021 ◽  
pp. 103905
Author(s):  
Qiling Chen ◽  
Xiangke Yang ◽  
Qiang Meng ◽  
Lili Zhao ◽  
Yuxin Yuan ◽  
...  
Keyword(s):  
Stress Response ◽  
Acid Stress ◽  
Oenococcus Oeni ◽  
Growth Stages ◽  
Acid Stress Response ◽  
Different Growth Stages

scholarly journals Differential View on the Bile Acid Stress Response of Clostridioides difficile

2019 ◽  
Vol 10 ◽  
Author(s):  
Susanne Sievers ◽  
Nicole G. Metzendorf ◽  
Silvia Dittmann ◽  
Daniel Troitzsch ◽  
Viola Gast ◽  
...  
Keyword(s):  
Bile Acid ◽  
Stress Response ◽  
Acid Stress ◽  
Clostridioides Difficile ◽  
Acid Stress Response

scholarly journals Mycobacterium tuberculosis virulence-regulator PhoP interacts with alternative sigma factor SigE during acid-stress response

2017 ◽  
Vol 104 (3) ◽  
pp. 400-411 ◽  
Author(s):  
Roohi Bansal ◽  
Vijjamarri Anil Kumar ◽  
Ritesh Rajesh Sevalkar ◽  
Prabhat Ranjan Singh ◽  
Dibyendu Sarkar
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Stress Response ◽  
Sigma Factor ◽  
Acid Stress ◽  
Alternative Sigma Factor ◽  
Acid Stress Response ◽  
Virulence Regulator

Transcriptome Analysis of the Acid Stress Response of Desulfovibrio vulgaris ATCC 7757

2020 ◽  
Vol 77 (10) ◽  
pp. 2702-2712
Author(s):  
Hang Yu ◽  
Zhiqiang Jiang ◽  
Yueer Lu ◽  
Xurong Yao ◽  
Chongyin Han ◽  
...  
Keyword(s):  
Stress Response ◽  
Transcriptome Analysis ◽  
Acid Stress ◽  
Desulfovibrio Vulgaris ◽  
Acid Stress Response

Involvement of the ornithine decarboxylase gene in acid stress response in probiotic Lactobacillus delbrueckii UFV H2b20

2015 ◽  
Vol 6 (5) ◽  
pp. 719-725 ◽  
Author(s):  
A.B. Ferreira ◽  
M.N.V. de Oliveira ◽  
F.S. Freitas ◽  
A.D. Paiva ◽  
P. Alfenas-Zerbini ◽  
...  
Keyword(s):  
Amino Acid ◽  
Stress Response ◽  
Intracellular Ph ◽  
Ornithine Decarboxylase ◽  
Ph Regulation ◽  
Acid Stress ◽  
Lactobacillus Delbrueckii ◽  
Control Treatment ◽  
Amino Acid Permease ◽  
Acid Stress Response

Amino acid decarboxylation is important for the maintenance of intracellular pH under acid stress. This study aims to carry out phylogenetic and expression analysis by real-time PCR of two genes that encode proteins involved in ornithine decarboxylation in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress. Sequencing and phylogeny analysis of genes encoding ornithine decarboxylase and amino acid permease in L. delbrueckii UFV H2b20 showed their high sequence identity (99%) and grouping with those of L. delbrueckii subsp. bulgaricus ATCC 11842. Exposure of L. delbrueckii UFV H2b20 cells in MRS pH 3.5 for 30 and 60 min caused a significant increase in expression of the gene encoding ornithine decarboxylase (up to 8.1 times higher when compared to the control treatment). Increased expression of the ornithine decarboxylase gene demonstrates its involvement in acid stress response in L. delbrueckii UFV H2b20, evidencing that the protein encoded by that gene could be involved in intracellular pH regulation. The results obtained show ornithine decarboxylation as a possible mechanism of adaptation to an acidic environmental condition, a desirable and necessary characteristic for probiotic cultures and certainly important to the survival and persistence of the L. delbrueckii UFV H2b20 in the human gastrointestinal tract.

The Lactic Acid Stress Response of Lactococcus lactis subsp. lactis

1996 ◽  
Vol 33 (3) ◽  
pp. 194-199 ◽  
Author(s):  
Axel Hartke ◽  
Sandrine Bouché ◽  
Jean-Christophe Giard ◽  
Abdellah Benachour ◽  
Philippe Boutibonnes ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Stress Response ◽  
Lactococcus Lactis ◽  
Acid Stress ◽  
Lactococcus Lactis Subsp ◽  
Acid Stress Response

scholarly journals Inactivation of PadR, the Repressor of the Phenolic Acid Stress Response, by Molecular Interaction with Usp1, a Universal Stress Protein from Lactobacillus plantarum, in Escherichia coli

2009 ◽  
Vol 75 (16) ◽  
pp. 5273-5283 ◽  
Author(s):  
Jérôme Gury ◽  
Hélène Seraut ◽  
Ngoc Phuong Tran ◽  
Lise Barthelmebs ◽  
Stéphanie Weidmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Lactobacillus Plantarum ◽  
Phenolic Acid ◽  
Stress Protein ◽  
Acid Stress ◽  
Universal Stress Protein ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Acid Stress Response

ABSTRACT The phenolic acid decarboxylase gene padA is involved in the phenolic acid stress response (PASR) in gram-positive bacteria. In Lactobacillus plantarum, the padR gene encodes the negative transcriptional regulator of padA and is cotranscribed with a downstream gene, usp1, which encodes a putative universal stress protein (USP), Usp1, of unknown function. The usp1 gene is overexpressed during the PASR. However, the role and the mechanism of action of the USPs are unknown in gram-positive bacteria. Therefore, to gain insights into the role of USPs in the PASR; (i) a usp1 deletion mutant was constructed; (ii) the two genes padR and usp1 were coexpressed with padA under its own promoter as a reporter gene in Escherichia coli; and (iii) molecular in vitro interactions between the PadR, Usp1, and the padA promoter were studied. Although the usp1 mutant strain retained phenolic acid-dependent PAD activity, it displayed a greater sensitivity to strong acidic conditions compared to that of the wild-type strain. PadR cannot be inactivated directly by phenolic acid in E. coli recombinant cultures but is inactivated by Usp1 when the two proteins are coexpressed in E. coli. The PadR inactivation observed in recombinant E. coli cells was supported by electrophoretic mobility shift assays. Although Usp1 seems not to be absolutely required for the PASR, its capacity to inactivate PadR indicates that it could serve as an important mediator in acid stress response mechanisms through its capacity to interact with transcriptional regulators.

Metabolomic analysis of acid stress response in Saccharomyces cerevisiae

2015 ◽  
Vol 120 (4) ◽  
pp. 396-404 ◽  
Author(s):  
Riyanto Heru Nugroho ◽  
Katsunori Yoshikawa ◽  
Hiroshi Shimizu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Acid Stress ◽  
Metabolomic Analysis ◽  
Acid Stress Response

scholarly journals Supramolecular assembly of the Escherichia coli LdcI upon acid stress

2020 ◽  
Vol 118 (2) ◽  
pp. e2014383118
Author(s):  
Matthew Jessop ◽  
Clarissa Liesche ◽  
Jan Felix ◽  
Ambroise Desfosses ◽  
Megghane Baulard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Fluorescent Protein ◽  
Mutational Analysis ◽  
Acid Stress ◽  
Low Ph ◽  
Supramolecular Organization ◽  
Cell Periphery ◽  
Wide Field ◽  
Acid Stress Response

Pathogenic and commensal bacteria often have to resist the harsh acidity of the host stomach. The inducible lysine decarboxylase LdcI buffers the cytosol and the local extracellular environment to ensure enterobacterial survival at low pH. Here, we investigate the acid stress-response regulation of Escherichia coli LdcI by combining biochemical and biophysical characterization with negative stain and cryoelectron microscopy (cryo-EM) and wide-field and superresolution fluorescence imaging. Due to deleterious effects of fluorescent protein fusions on native LdcI decamers, we opt for three-dimensional localization of nanobody-labeled endogenous wild-type LdcI in acid-stressed E. coli cells and show that it organizes into distinct patches at the cell periphery. Consistent with recent hypotheses that in vivo clustering of metabolic enzymes often reflects their polymerization as a means of stimulus-induced regulation, we show that LdcI assembles into filaments in vitro at physiologically relevant low pH. We solve the structures of these filaments and of the LdcI decamer formed at neutral pH by cryo-EM and reveal the molecular determinants of LdcI polymerization, confirmed by mutational analysis. Finally, we propose a model for LdcI function inside the enterobacterial cell, providing a structural and mechanistic basis for further investigation of the role of its supramolecular organization in the acid stress response.

Sign in / Sign up

Export Citation Format

Share Document