The Lactic Acid Stress Response of Lactococcus lactis subsp. lactis

1996 ◽  
Vol 33 (3) ◽  
pp. 194-199 ◽  
Author(s):  
Axel Hartke ◽  
Sandrine Bouché ◽  
Jean-Christophe Giard ◽  
Abdellah Benachour ◽  
Philippe Boutibonnes ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Stress Response ◽  
Lactococcus Lactis ◽  
Acid Stress ◽  
Lactococcus Lactis Subsp ◽  
Acid Stress Response

scholarly journals RECTA: Regulon Identification Based on Comparative Genomics and Transcriptomics Analysis

2018 ◽  
Author(s):  
Xin Chen ◽  
Anjun Ma ◽  
Adam McDermaid ◽  
Hanyuan Zhang ◽  
Chao Liu ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Stress Response ◽  
Comparative Genomics ◽  
Gene Regulatory Network ◽  
Lactococcus Lactis ◽  
Regulatory Network ◽  
Acid Stress ◽  
Orthologous Genes ◽  
Gene Regulatory ◽  
Acid Stress Response

ABSTRACTRegulons, which serve as co-regulated gene groups contributing to the transcriptional regulation of microbial genomes, have the potential to aid in understanding of underlying regulatory mechanisms. In this study, we designed a novel computational pipeline, RECTA, for regulon prediction related to the gene regulatory network under certain conditions. To demonstrate the effectiveness of this tool, we implemented RECTA onLactococcus lactisMG1363 data to elucidate acid-response regulons.Lactococcus lactisis one of the most important Gram-positive lactic acid-producing bacteria, widely used in food industry and has been proved to have advantages in oral delivery of drug and vaccine. The pipeline carries out differential gene expression, gene co-expression analysis,cis-regulatory motif finding, and comparative genomics to predict and validate regulons related to acid stress response. A total of 51 regulonswere identified, 14 of which have computational-verified significance. Among these 14 regulons, five of them were computationally predicted to be connected with acid stress response with (i) known transcriptional factors in MEME suite database successfully mapped inLactococcus lactisMG1363; and (ii) differentially expressed genes between pH values of 6.5 (control) and 5.1 (treatment). Validated by 36 literature confirmed acid stress response related proteins and genes, 33 genes inLactococcus lactisMG1363 were found having orthologous genes using BLAST, associated to six regulons. An acid response related regulatory network was constructed, involving two trans-membrane proteins, eight regulons (llrA, llrC, hllA, ccpA, NHP6A,rcfB, regulons #8 and #39), nine functional modules, and 33 genes with orthologous genes known to be associated to acid stress. Our RECTA pipeline provides an effective way to construct a reliable gene regulatory network through regulon elucidation. The predicted response pathways could serve as promising candidates for better acid tolerance engineering inLactococcus lactis. RECTA has strong application power and can be effectively applied to other bacterial genomes where the elucidation of the transcriptional regulation network is needed.

Acid stress response in environmental and clinical strains of enteric bacteria

2012 ◽  
Vol 7 (6) ◽  
pp. 495-505 ◽  
Author(s):  
Gabriel J. Swenson ◽  
J. Stochastic ◽  
Franklyn F. Bolander ◽  
Richard A. Long
Keyword(s):  
Stress Response ◽  
Acid Stress ◽  
Enteric Bacteria ◽  
Clinical Strains ◽  
Acid Stress Response

Integrative multiomics analysis of the acid stress response of Oenococcus oeni mutants at different growth stages

2021 ◽  
pp. 103905
Author(s):  
Qiling Chen ◽  
Xiangke Yang ◽  
Qiang Meng ◽  
Lili Zhao ◽  
Yuxin Yuan ◽  
...  
Keyword(s):  
Stress Response ◽  
Acid Stress ◽  
Oenococcus Oeni ◽  
Growth Stages ◽  
Acid Stress Response ◽  
Different Growth Stages

scholarly journals Differential View on the Bile Acid Stress Response of Clostridioides difficile

2019 ◽  
Vol 10 ◽  
Author(s):  
Susanne Sievers ◽  
Nicole G. Metzendorf ◽  
Silvia Dittmann ◽  
Daniel Troitzsch ◽  
Viola Gast ◽  
...  
Keyword(s):  
Bile Acid ◽  
Stress Response ◽  
Acid Stress ◽  
Clostridioides Difficile ◽  
Acid Stress Response

scholarly journals Mycobacterium tuberculosis virulence-regulator PhoP interacts with alternative sigma factor SigE during acid-stress response

2017 ◽  
Vol 104 (3) ◽  
pp. 400-411 ◽  
Author(s):  
Roohi Bansal ◽  
Vijjamarri Anil Kumar ◽  
Ritesh Rajesh Sevalkar ◽  
Prabhat Ranjan Singh ◽  
Dibyendu Sarkar
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Stress Response ◽  
Sigma Factor ◽  
Acid Stress ◽  
Alternative Sigma Factor ◽  
Acid Stress Response ◽  
Virulence Regulator

The Citrate Transport System of Lactococcus lactis subsp. lactis biovar diacetylactis Is Induced by Acid Stress

1998 ◽  
Vol 64 (3) ◽  
pp. 850-857 ◽  
Author(s):  
Nieves García-Quintáns ◽  
Christian Magni ◽  
Diego de Mendoza ◽  
Paloma López
Keyword(s):  
Stress Response ◽  
Cell Growth ◽  
Lactococcus Lactis ◽  
Growth Medium ◽  
Transport System ◽  
Acid Stress ◽  
Selective Advantage ◽  
Transcriptional Level ◽  
Transport Activity ◽  
Citrate Transport

ABSTRACT Citrate transport in Lactococcus lactis subsp.lactis biovar diacetylactis is catalyzed by citrate permease P (CitP), which is encoded by the plasmidic citPgene. We have shown previously that citP is included in thecitQRP operon, which is mainly transcribed from the P1 promoter in L. lactis subsp. lactis biovar diacetylactis. Furthermore, transcription of citQRP and citrate transport are not induced by the presence of citrate in the growth medium. In this work, we analyzed the influence of the extracellular pH on the expression of citP. The citrate transport system is induced by natural acidification of the medium during cell growth and by a shift to media buffered at acidic pHs. This inducible response to acid stress takes place at the transcriptional level and seems to be due to increased utilization of the P1 promoter. Increased transcription correlates with increased synthesis of CitP and results in higher citrate transport activity catalyzed by the cells. Finally, this acid stress response seems to provide L. lactis subsp. lactis biovar diacetylactis with a selective advantage resulting from cometabolism of glucose and citrate at low pHs.

Transcriptome Analysis of the Acid Stress Response of Desulfovibrio vulgaris ATCC 7757

2020 ◽  
Vol 77 (10) ◽  
pp. 2702-2712
Author(s):  
Hang Yu ◽  
Zhiqiang Jiang ◽  
Yueer Lu ◽  
Xurong Yao ◽  
Chongyin Han ◽  
...  
Keyword(s):  
Stress Response ◽  
Transcriptome Analysis ◽  
Acid Stress ◽  
Desulfovibrio Vulgaris ◽  
Acid Stress Response

scholarly journals Genome Sequence of the Lactic Acid Bacterium Lactococcus lactis subsp. lactis TOMSC161, Isolated from a Nonscalded Curd Pressed Cheese

2014 ◽  
Vol 2 (6) ◽  
Author(s):  
H. Velly ◽  
P. Renault ◽  
A.- L. Abraham ◽  
V. Loux ◽  
A. Delacroix-Buchet ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Lactococcus Lactis ◽  
Genome Sequence ◽  
Lactococcus Lactis Subsp

Involvement of the ornithine decarboxylase gene in acid stress response in probiotic Lactobacillus delbrueckii UFV H2b20

2015 ◽  
Vol 6 (5) ◽  
pp. 719-725 ◽  
Author(s):  
A.B. Ferreira ◽  
M.N.V. de Oliveira ◽  
F.S. Freitas ◽  
A.D. Paiva ◽  
P. Alfenas-Zerbini ◽  
...  
Keyword(s):  
Amino Acid ◽  
Stress Response ◽  
Intracellular Ph ◽  
Ornithine Decarboxylase ◽  
Ph Regulation ◽  
Acid Stress ◽  
Lactobacillus Delbrueckii ◽  
Control Treatment ◽  
Amino Acid Permease ◽  
Acid Stress Response

Amino acid decarboxylation is important for the maintenance of intracellular pH under acid stress. This study aims to carry out phylogenetic and expression analysis by real-time PCR of two genes that encode proteins involved in ornithine decarboxylation in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress. Sequencing and phylogeny analysis of genes encoding ornithine decarboxylase and amino acid permease in L. delbrueckii UFV H2b20 showed their high sequence identity (99%) and grouping with those of L. delbrueckii subsp. bulgaricus ATCC 11842. Exposure of L. delbrueckii UFV H2b20 cells in MRS pH 3.5 for 30 and 60 min caused a significant increase in expression of the gene encoding ornithine decarboxylase (up to 8.1 times higher when compared to the control treatment). Increased expression of the ornithine decarboxylase gene demonstrates its involvement in acid stress response in L. delbrueckii UFV H2b20, evidencing that the protein encoded by that gene could be involved in intracellular pH regulation. The results obtained show ornithine decarboxylation as a possible mechanism of adaptation to an acidic environmental condition, a desirable and necessary characteristic for probiotic cultures and certainly important to the survival and persistence of the L. delbrueckii UFV H2b20 in the human gastrointestinal tract.

scholarly journals Inactivation of PadR, the Repressor of the Phenolic Acid Stress Response, by Molecular Interaction with Usp1, a Universal Stress Protein from Lactobacillus plantarum, in Escherichia coli

2009 ◽  
Vol 75 (16) ◽  
pp. 5273-5283 ◽  
Author(s):  
Jérôme Gury ◽  
Hélène Seraut ◽  
Ngoc Phuong Tran ◽  
Lise Barthelmebs ◽  
Stéphanie Weidmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Lactobacillus Plantarum ◽  
Phenolic Acid ◽  
Stress Protein ◽  
Acid Stress ◽  
Universal Stress Protein ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Acid Stress Response

ABSTRACT The phenolic acid decarboxylase gene padA is involved in the phenolic acid stress response (PASR) in gram-positive bacteria. In Lactobacillus plantarum, the padR gene encodes the negative transcriptional regulator of padA and is cotranscribed with a downstream gene, usp1, which encodes a putative universal stress protein (USP), Usp1, of unknown function. The usp1 gene is overexpressed during the PASR. However, the role and the mechanism of action of the USPs are unknown in gram-positive bacteria. Therefore, to gain insights into the role of USPs in the PASR; (i) a usp1 deletion mutant was constructed; (ii) the two genes padR and usp1 were coexpressed with padA under its own promoter as a reporter gene in Escherichia coli; and (iii) molecular in vitro interactions between the PadR, Usp1, and the padA promoter were studied. Although the usp1 mutant strain retained phenolic acid-dependent PAD activity, it displayed a greater sensitivity to strong acidic conditions compared to that of the wild-type strain. PadR cannot be inactivated directly by phenolic acid in E. coli recombinant cultures but is inactivated by Usp1 when the two proteins are coexpressed in E. coli. The PadR inactivation observed in recombinant E. coli cells was supported by electrophoretic mobility shift assays. Although Usp1 seems not to be absolutely required for the PASR, its capacity to inactivate PadR indicates that it could serve as an important mediator in acid stress response mechanisms through its capacity to interact with transcriptional regulators.

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