scholarly journals Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development

2019 ◽  
Vol 55 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Kinga Dziurka ◽  
Michał Dziurka ◽  
Marzena Warchoł ◽  
Ilona Czyczyło-Mysza ◽  
Izabela Marcińska ◽  
...  
2008 ◽  
Vol 36 (1) ◽  
pp. 43-51 ◽  
Author(s):  
S. Prodanovic ◽  
F. Matzk ◽  
D. Zoric

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinga Dziurka ◽  
Michał Dziurka ◽  
Ewa Muszyńska ◽  
Ilona Czyczyło-Mysza ◽  
Marzena Warchoł ◽  
...  

AbstractA critical step in the production of doubled haploids is a conversion of the haploid embryos into plants. Our study aimed to recognize the reasons for the low germination rate of Avena sativa haploid embryos obtained by distant crossing with maize. Oat cultivars of ‘Krezus’ and ‘Akt’ were investigated regarding embryo anatomy, the endogenous phytohormone profiles, and antioxidant capacity. The zygotic embryos of oat were used as a reference. It was found that twenty-one days old haploid embryos were smaller and had a less advanced structure than zygotic ones. Morphology and anatomy modifications of haploid embryos were accompanied by extremely low levels of endogenous auxins. Higher levels of cytokinins, as well as tenfold higher cytokinin to auxin ratio in haploid than in zygotic embryos, may suggest an earlier stage of development of these former. Individual gibberellins reached higher values in ‘Akt’ haploid embryos than in the respective zygotic ones, while the differences in both types of ‘Krezus’ embryos were not noticed. Additionally to the hormonal regulation of haploid embryogenesis, the poor germination of oat haploid embryos can be a result of the overproduction of reactive oxygen species, and therefore higher levels of low molecular weight antioxidants and stress hormones.


Author(s):  
B. K. Kirchoff ◽  
L.F. Allard ◽  
W.C. Bigelow

In attempting to use the SEM to investigate the transition from the vegetative to the floral state in oat (Avena sativa L.) it was discovered that the procedures of fixation and critical point drying (CPD), and fresh tissue examination of the specimens gave unsatisfactory results. In most cases, by using these techniques, cells of the tissue were collapsed or otherwise visibly distorted. Figure 1 shows the results of fixation with 4.5% formaldehyde-gluteraldehyde followed by CPD. Almost all cellular detail has been obscured by the resulting shrinkage distortions. The larger cracks seen on the left of the picture may be due to dissection damage, rather than CPD. The results of observation of fresh tissue are seen in Fig. 2. Although there is a substantial improvement over CPD, some cell collapse still occurs.Due to these difficulties, it was decided to experiment with cold stage techniques. The specimens to be observed were dissected out and attached to the sample stub using a carbon based conductive paint in acetone.


Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
S Chon ◽  
R Earland ◽  
A Pappas ◽  
KA Reynertson ◽  
MD Southall

Author(s):  
Francisco Gavi Reyes ◽  
César Botello-Aguillón ◽  
Leonardo Tijerina-Chávez ◽  
Arturo Galvis-Spíndola ◽  
Rodrigo Roblero-Hidalgo

E Objetivo: Desarrollar un procedimiento para estimar biomasa con imágenes digitales captadas desde un dron y modelación 3D (ID-Dron-3D) aplicable en alfalfa (Medicago sativa L.) y avena forrajera (Avena sativa L.). Diseño/metodología/aproximación: Con una cámara digital acoplada al dron se obtuvieron imágenes antes de la cosecha de los cultivos, que fueron procesadas con software para luego estimar volumen de biomasa. En cada cultivo se midió altura de la planta y área cosechada, volumen aparente y real de biomasa, y peso de biomasa fresca y seca. Resultados: Con base en el análisis de regresión se obtuvieron modelos lineales a una p<0.05 para predecir: biomasa fresca en avena (R2=0.70) y alfalfa (R2 =0.47); y biomasa seca en avena (R2=0.78) y en alfalfa (R2=0.31) mediante ID-Dron-3D. Limitaciones del estudio/implicaciones: Considerando las R2 de los modelos obtenidos, los resultados en la avena forrajera fueron mejores, respecto a los detectados en alfalfa, lo cual se puede deber a la mayor variabilidad de la cobertura vegetal, ya que, en algunas unidades de muestreo, las plantas de alfalfa no cubrían completamente el suelo. Hallazgos/conclusiones: El rendimiento de biomasa fresca y seca de ambos cultivos se correlacionó significativamente con su respectivo volumen aparente estimado con imágenes digitales tomadas desde un dron y su procesamiento 3D (ID-Dron-3D).


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