scholarly journals Anatomical and hormonal factors determining the development of haploid and zygotic embryos of oat (Avena sativa L.)

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinga Dziurka ◽  
Michał Dziurka ◽  
Ewa Muszyńska ◽  
Ilona Czyczyło-Mysza ◽  
Marzena Warchoł ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Hormonal Regulation ◽  
Germination Rate ◽  
Doubled Haploids ◽  
Stress Hormones ◽  
Zygotic Embryos ◽  
Stage Of Development ◽  
Oat Cultivars ◽  
Endogenous Phytohormone ◽  
Haploid Embryos

AbstractA critical step in the production of doubled haploids is a conversion of the haploid embryos into plants. Our study aimed to recognize the reasons for the low germination rate of Avena sativa haploid embryos obtained by distant crossing with maize. Oat cultivars of ‘Krezus’ and ‘Akt’ were investigated regarding embryo anatomy, the endogenous phytohormone profiles, and antioxidant capacity. The zygotic embryos of oat were used as a reference. It was found that twenty-one days old haploid embryos were smaller and had a less advanced structure than zygotic ones. Morphology and anatomy modifications of haploid embryos were accompanied by extremely low levels of endogenous auxins. Higher levels of cytokinins, as well as tenfold higher cytokinin to auxin ratio in haploid than in zygotic embryos, may suggest an earlier stage of development of these former. Individual gibberellins reached higher values in ‘Akt’ haploid embryos than in the respective zygotic ones, while the differences in both types of ‘Krezus’ embryos were not noticed. Additionally to the hormonal regulation of haploid embryogenesis, the poor germination of oat haploid embryos can be a result of the overproduction of reactive oxygen species, and therefore higher levels of low molecular weight antioxidants and stress hormones.

scholarly journals Application of chosen factors in the wide crossing method for the production of oat doubled haploids

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Agata Nowakowska ◽  
Edyta Skrzypek ◽  
Izabela Marcińska ◽  
Ilona Czyczyło-Mysza ◽  
Kinga Dziurka ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Doubled Haploids ◽  
Sugar Content ◽  
Colchicine Treatment ◽  
High Survival ◽  
High Survival Rate ◽  
Natural Conditions ◽  
Maize Pollen ◽  
Dh Lines ◽  
Haploid Embryos

AbstractOat (Avena sativa L.) has recently gained importance due to the discovery of a variety of health benefits and new opportunities of use. There is no efficient protocol for the production of oat doubled haploid (DH) lines. The aim of this study was to increase the efficiency of obtaining DHs of oat by the wide crossing method. The study was performed on five oat genotypes. We have compared the induction of embryos after pollination with maize, sorghum and millet pollen as well as the development of haploid embryos isolated 2, 3 and 4 weeks after pollination and cultivated on media with different sugar content. Haploid plants were treated with colchicine after or before acclimation to natural conditions. Of the three types of pollen used, the largest number of haploid embryos was obtained using maize pollen. Three weeks after pollination was the most suitable time for the isolation and cultivation of the embryos. The most efficient medium enabling the development of embryos and conversion to plants was 190-2 containing 9% of maltose. Colchicine treatment of acclimated plants provided high survival rate.

scholarly journals Adaptability and stability of white oat cultivars in relation to chemical composition of the caryopsis

2013 ◽  
Vol 48 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Maraisa Crestani Hawerroth ◽  
Fernando Irajá Félix de Carvalho ◽  
Antonio Costa de Oliveira ◽  
José Antonio Gonzalez da Silva ◽  
Luiz Carlos Gutkoski ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Avena Sativa ◽  
Block Design ◽  
Chemical Properties ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Average Performance ◽  
Randomized Block Design ◽  
Nitrogen Free Extract ◽  
Oat Cultivars

The objective of this work was to characterize the chemical properties of white oat (Avena sativa) caryopsis and to determine the adaptability and stability of cultivars recommended for cultivation in the state of Rio Grande do Sul, Brazil. The trials were carried out in the 2007, 2008 and 2009 crop seasons, in three municipalities: Augusto Pestana, Capão do Leão, and Passo Fundo. Fifteen cultivars were evaluated in a randomized block design, with four replicates. The contents of protein, lipid, and nitrogen-free extract were evaluated in the caryopsis. Cultivar performances for the measured characters varied according to location and year of cultivation. The cultivar URS Guapa showed high content of nitrogen-free extract and low contents of protein and lipid in the caryopsis. 'FAPA Louise' showed high content of lipid, whereas 'Albasul', 'UPF 15', and 'UPF 18' showed high content of protein and low content of nitrogen-free extract. There is no evidence of an ideal biotype for the evaluated characters, which could simultaneously show high average performance, adaptability to favorable and unfavorable environments, and stability.

THE ROLE OF TRANSPOSABLE ELEMENTS IN ENDOCRINE CHANGES DURING AGING

2020 ◽  
pp. 418-428
Author(s):  
Р. Н. Мустафин ◽  
Э. К. Хуснутдинова
Keyword(s):  
Hormonal Regulation ◽  
Endocrine System ◽  
Cell Types ◽  
Key Factors ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Stage Of Development ◽  
Expression Of Genes ◽  
Transposon Activity ◽  
Species Specific

Одним из ключевых механизмов старения является изменение гормональной регуляции, для эффективного воздействия на которую с целью продления жизни необходимо определение первопричины данных процессов. В качестве молекулярных драйверов, управляющих динамикой уровня гормонов, могут служить транспозоны. Это связано с их использованием в качестве источников нуклеотидных последовательностей, воспринимающих специфические сигналы рибозимов, транскрипционных факторов, гормонов и их мессенджеров. В то же время, в эволюции транспозоны являются источниками рибозимов и белков, обладающих ДНК-связывающими доменами. Начиная с деления зиготы, видоспецифический состав и распределение транспозонов в геноме могут использоваться как биологическая кодировка, необходимая для последовательной и специфической для типов клеток экспрессии генов. Сделано предположение, что гормональная регуляция является одним из компонентов сложной системы управления онтогенезом под влиянием мобильных элементов. В качестве подтверждения приведены работы о роли транспозонов в управлении генами эндокринной системы, а также о влиянии гормонов на активность транспозонов. Исследование этих взаимосвязей может иметь перспективы для разработки методов продления жизни, так как эпигенетические изменения под влиянием транспозонов носят обратимый характер. Species-specific changes in the endocrine system are key factors in aging. Therefore, to prolong life, it is necessary to find regulators of the highest level, the changes of which lead to physiological aging. The molecular drivers that control dynamics of hormone levels can be transposons. This is due to the use of nucleotide sequences of transposons as binding sites that perceive specific signals of ribozymes, transcription factors, hormones and their messengers. At the same time, transposons are evolutionary sources of ribozymes and proteins that have DNA-binding domains. Starting from zygote division, the species-specific composition and distribution of transposons in the genome serves as a biological coding, which is necessary for the sequential expression of genes specific to cell types and stage of development. We suggest that hormonal regulation is one of the components of this complex system of regulation of ontogenesis under the control of transposons. To confirm our hypothesis, this review contains articles that prove the importance of transposons for species-specific control of endocrine system genes, as well as the effect of hormones on transposon activity. The research of these relationships is promising for the development of methods for the effective prolongation of life, since epigenetic changes under the influence of transposons are reversible.

scholarly journals Phosphorus requirement and partitioning in oat cultivars

2019 ◽  
Vol 49 (2) ◽  
Author(s):  
Jonas Manica ◽  
Pedro Alexandre Varella Escosteguy
Keyword(s):  
Avena Sativa ◽  
Cover Crop ◽  
Dry Matter ◽  
Phosphorus Content ◽  
Nutrient Requirements ◽  
Physiological Maturity ◽  
P Content ◽  
Phosphorus Requirement ◽  
Oat Cultivars ◽  
Dry Matter Weight

ABSTRACT: Maintenance fertilization changes with nutrient requirements and partitioning, but these are not well known for oat cultivars. The aim of this study was to evaluate whether phosphorus (P) requirements and partitioning vary among cultivars of oat (Avena sativa L.). The UPFA ‘Ouro’, UPFPS ‘Farroupilha’, IPR ‘Afrodite’, URS ‘FAPA Slava’, ‘Barbarasul’, ‘FAEM 007’, URS ‘Taura’, and ‘URS 21’ were cultivated in a Latosol with high P content in a greenhouse in 2015. Plants were sampled at physiological maturity and divided into roots, stem, leaves, and grain tissues. Phosphorus content and the dry matter weight of these parts were evaluated and the uptake amount and partitioning of P were estimated. The values of these variables differed across cultivars, except the P content in the grain (‘average’, 4.0g P kg-1). ‘Afrodite’ and ‘FAPA Slava’ have lower requirements and partitioning of P in the grain, but these did not vary among the other cultivars. Requirements and partitioning of P in stem and leaves increased in the following order: ‘Ouro’ and ‘Farroupilha’<‘Taura’ and ‘FAPA Slava’<‘URS 21’<‘Afrodite’, ‘Barbarasul’ and ‘FAEM 007’. Requirement and partitioning of P in roots, stem, leaves, and grain differs across cultivars, which indicates that this factor, besides how the oat is used (such as for grain, pasture, silage, cover crop), can be used to improve maintenance fertilization.

AC Morgan Oat

2001 ◽  
Vol 81 (1) ◽  
pp. 85-87 ◽  
Author(s):  
Solomon Kibite ◽  
James G. Menzies
Keyword(s):  
Key Words ◽  
Avena Sativa ◽  
Oil Content ◽  
Research Centre ◽  
High Protein Content ◽  
Desirable Feature ◽  
Cultivar Description ◽  
Oat Cultivars ◽  
Milling Yield ◽  
High Test

AC Morgan is a high-yielding spring oat (Avena sativa L.) cultivar developed by Agriculture and Agri-Food Canada, Lacombe Research Centre, Lacombe, Alberta and released in 1999. It is described as a medium maturing cultivar with strong straw, plump kernels, high test weight, high protein content, low hull content and high milling yield. It also has low oil content, which is a desirable feature in milling oat cultivars. AC Morgan is well adapted to Alberta and the rust-free areas of Saskatchewan. Key words: Avena sativa, oat (spring), cultivar description

scholarly journals Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media

2016 ◽  
Vol 52 (6) ◽  
pp. 590-597 ◽  
Author(s):  
Angelika Noga ◽  
Edyta Skrzypek ◽  
Marzena Warchoł ◽  
Ilona Czyczyło-Mysza ◽  
Kinga Dziurka ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Avena Sativa ◽  
Haploid Embryos

scholarly journals Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development

2019 ◽  
Vol 55 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Kinga Dziurka ◽  
Michał Dziurka ◽  
Marzena Warchoł ◽  
Ilona Czyczyło-Mysza ◽  
Izabela Marcińska ◽  
...  
Keyword(s):  
Embryo Development ◽  
Avena Sativa ◽  
Haploid Embryo ◽  
Endogenous Phytohormone

Oat β-glucans: An evaluation of eastern Canadian cultivars and unregistered lines

1993 ◽  
Vol 73 (2) ◽  
pp. 429-436 ◽  
Author(s):  
S. S. Miller ◽  
R. G. Fulcher ◽  
D. J. Vincent ◽  
J. Weisz
Keyword(s):  
Content Analysis ◽  
Environmental Factors ◽  
Avena Sativa ◽  
Rank Order ◽  
Positive Association ◽  
Kernel Weight ◽  
Negative Association ◽  
Significant Positive Association ◽  
Factors Influencing ◽  
Oat Cultivars

The (1-3), (1-4)-β-D-glucan (β-glucan) content of a number of domestic Canadian oat cultivars and selected unregistered lines was determined to establish the range of β-glucan content in eastern Canadian oat varieties. Seed samples were taken from oats grown at five locations over 3 years in an attempt to assess the effect of environment on variation in β-glucan content. Analysis of variance indicated that the greater source of variation in β-glucan content was due to genetic rather than environmental factors. The highest β-glucan cultivar (Marion) was about 30% higher than the lowest cultivars (OA516-2 and Donald). Differences in β-glucan content among the intermediate cultivars were generally smaller, and in some cases not significant, although the rank order of the cultivars among environments was consistent. A low, but significant, negative association between β-glucan content and precipitation, and a low, but significant, positive association between β-glucan content and temperature was found, but these were not dominant factors influencing β-glucan levels in oats. There was no consistent association between β-glucan content and protein, oil, thousand kernel weight or grain yield (kg/hectare). Key words: Oat, Avena sativa, β-glucan, variation

scholarly journals Plant Regeneration via Somatic Embryogenesis in Larix principis-rupprechtii Mayr

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1335
Author(s):  
Shuaifei Jiang ◽  
Xiaoyi Chen ◽  
Ying Gao ◽  
Ying Cui ◽  
Lisheng Kong ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
High Efficiency ◽  
Germination Rate ◽  
Culture Method ◽  
Culture Conditions ◽  
Zygotic Embryos ◽  
Multiplication Rate ◽  
Embryo Maturation ◽  
Somatic Embryo Maturation

Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr) is a native conifer in North China with great economic and ecological values. Somatic embryogenesis (SE) is a powerful tool for the mass clonal propagation in plants. In this study, we described a high-efficiency SE system via indirect pathways and investigated the effect of genotype, culture conditions and phytohormones on SE. Immature zygotic embryos (IZEs) of L. principis-rupprechtii Mayr were used as explant materials. In the induction stage, embryogenic tissues (ETs) were induced on mLV medium supplemented with 2.0 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg L−1 6-benzylaminopurine (6-BA). The initiation frequencies showed significant differences (p < 0.05) among 20 genotypes of open-pollinated mother trees with the highest induction frequency reaching 30%. For tissue proliferation, proliferation in liquid medium was more efficient compared with proliferation in semi-solid medium, providing a multiplication rate of 3.12 in an 8-day subculture period. As a necessary exogenous plant growth regulator (PGR) for somatic embryo maturation in conifers, abscisic acid (ABA) was optimized at 16 mg L−1 in this system. Next, an orthogonal test on osmotic pressure factors showed 50 g L−1 sucrose, 7 g L−1 phytagel and 75 g L−1 polyethylene glycol (PEG) was the optimal combination for somatic embryo maturation in L. principis-rupprechtii Mayr. Moreover, the dispersion culture method provided a more efficient somatic embryo maturation, up to 545 per gram of fresh weight (FW). Finally, 2 g L−1 of active charcoal (AC) was found to increase the somatic embryo germination rate to 63.46%. The improved protocol of SE will serve as a foundation for establishing mass propagation and genetic transformation of L. principis-rupprechtii Mayr.

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