In vitro morpho-physiological performance and DNA stability of banana under cadmium and drought stresses

2021 ◽  
Author(s):  
Marwa T. El-Mahdy ◽  
Dalia A. Abdel-Wahab ◽  
Muhammad Youssef
Keyword(s):  
Dna Stability ◽  
Physiological Performance

DNA stability and lipid peroxidation in vitamin E–deficient rats in vivo and colon cells in vitro

2004 ◽  
Vol 44 (4) ◽  
pp. 195-203 ◽  
Author(s):  
S. J. Duthie ◽  
P. T. Gardner ◽  
P. C. Morrice ◽  
S. G. Wood ◽  
L. Pirie ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin E ◽  
Dna Stability ◽  
Colon Cells

In vitro physiological performance factors of a catalase-based biosensor for real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals

2016 ◽  
Vol 8 (42) ◽  
pp. 7614-7622 ◽  
Author(s):  
Saidhbhe L. O'Riordan ◽  
Kelly Mc Laughlin ◽  
John P. Lowry
Keyword(s):  
Hydrogen Peroxide ◽  
Real Time ◽  
Electrochemical Detection ◽  
Physiological Performance ◽  
Performance Factors ◽  
Freely Moving

The in vitro physiological performance factors of a catalase-based microelectrochemical biosensor, developed for real-time neurochemical monitoring of hydrogen peroxide.

scholarly journals High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana

2019 ◽  
Vol 139 (3) ◽  
pp. 563-575
Author(s):  
M. Pavicic ◽  
F. Wang ◽  
K. Mouhu ◽  
K. Himanen
Keyword(s):  
High Throughput ◽  
Genetic Background ◽  
Seed Quality ◽  
Quality Attributes ◽  
Adult Plant ◽  
In Vitro Germination ◽  
E3 Ligases ◽  
Ring Type ◽  
Physiological Performance

Abstract Seed quality is an important factor for seedling vigour as well as adult plant resilience. The key quality attributes are related to physical characteristics, physiological performance, genetic background and health status of the seeds. Many ways to address seed quality attributes have been developed and recently many of them have featured automated high throughput methods. In our study, we addressed two of the seed quality attributes, namely physiological performance and genetic background by analysing germination rates in our mutant collection. These mutants represent ubiquitin E3 ligases that transcriptionally respond to abscisic acid (ABA). This plant hormone is an important regulator of germination and seedling establishment. To facilitate in vitro germination screens of large seed collections a high throughput image-based assay was developed. As a read out of the germination on ABA treatment the cotyledon emergence was detected with top view chlorophyll fluorescence camera. By applying the ABA treatment during germination, RING-type ubiquitin E3 ligase mutants were identified, showing either resistant or sensitive responses to ABA. In conclusion, a scalable high throughput screen for in vitro germination assay was established that allowed fast screening of tens of mutants in a hormone supplemented media.

scholarly journals Parvovirus uncoating in vitro reveals a mechanism of DNA release without capsid disassembly and striking differences in encapsidated DNA stability

Virology ◽  
2006 ◽  
Vol 345 (1) ◽  
pp. 137-147 ◽  
Author(s):  
Carlos Ros ◽  
Claudia Baltzer ◽  
Bernhard Mani ◽  
Christoph Kempf
Keyword(s):  
Dna Stability ◽  
Dna Release ◽  
Capsid Disassembly

Oxidative Stress-induced Toxicity and DNA Stability in Some Agri-field Based Livestock/Insect by Widely used Pesticides

2020 ◽  
Vol 23 (9) ◽  
pp. 972-980 ◽  
Author(s):  
Balaram Manna ◽  
Sangita M. Dutta ◽  
Subrata Dalapati ◽  
Smarajit Maiti
Keyword(s):  
Agricultural Productivity ◽  
Enzymatic Activities ◽  
Marker Enzymes ◽  
Pest Population ◽  
Dna Stability ◽  
Poultry Birds ◽  
In Vitro Exposure ◽  
Chemical Cycle ◽  
Insect Gut

Aim and Objectives: Humans continuously use pesticides in the field to control the pest population and weeds for considerable agricultural productivity. Side-by species like grazinganimals, insects and other species are adversely affected by or become resistant to pesticides. Insects, birds and cattle are highly abundant dwellers of the agriculture-field and represent three distinct phyla having versatile physiological features. Besides higher agricultural-productivity, protection to several species will maintain ecological/environmental balance. Studies on the effect of widely used pesticides on their DNA-stability and important enzymatic-activities are insufficient. Materials and Methods: Antioxidant-activity (Superoxide-dismutase; SOD/Catalase- by gelzymogram- assay) and DNA-stability (fragmentation-assay) in hepatic/gut tissues were studied after in vitro exposure of Chlorpyrifos, Fenvalerate, Nimbecidine or Azadirachtin to goat/cow/poultry-hen/insect. Results: In general, all pesticides were found to impair enzymatic-activities. However, lower organisms were affected more than higher vertebrates by azadirachtin-treatment. DNA fragmentation was found more in insects/poultry-birds than that of the cattle in hepatic/gut tissues. Inversely, toxicity/antioxidant marker-enzymes were more responsive in insect gut-tissues. However, mitochondrialtoxicity revealed variable effects on different species. It has been noticed that chlorpyrifos is the most toxic pesticide, followed by Fenvalerate/Nimbecidine (Azadirachtin, AZT). Nevertheless, AZT revealed its higher DNA-destabilizing effects on the field-insects as compared to the other animals. Conclusion: Field-insects are highly integrated into the ecosystem and the local bio-geo-chemical cycle, which may be impaired. Pesticides may have toxic effects on higher vertebrates and may sustain in the soil after being metabolized into their different derivatives. Some of the sensitive biochemical parameters of this organism may be used as a biomarker for pesticide toxicity.

scholarly journals Evaluation of plasmid DNA stability against ultrasonic shear stress and its in vitro delivery efficiency using ionic liquid [Bmim][PF6]

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 29225-29231 ◽  
Author(s):  
Satya Ranjan Sarker ◽  
Andrew S. Ball ◽  
Suresh K. Bhargava ◽  
Sarvesh K. Soni
Keyword(s):  
Shear Stress ◽  
Ionic Liquid ◽  
Plasmid Dna ◽  
Electrostatic Interactions ◽  
Dna Stability ◽  
Functional Nanostructures ◽  
Hydrophobic Ionic Liquid ◽  
Ultrasonic Shear ◽  
Delivery Efficiency

The hydrophobic ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate [Bmim][PF6] forms ultrasonically stable and functional nanostructures with negatively charged plasmid DNA through electrostatic interactions.

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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