Genetic diversity and differentiation of pedunculate (Quercus robur) and sessile (Q. petraea) oaks

Abstract This study was conducted to determine the parent–offspring genetic structure of the pedunculate oak (Quercus robur L.), sessile oak (Q. petraea [Matt.] Liebl.) and their hybrids. Forty half-sib Quercus families and their maternal trees originating from one tree stand in southern Lithuania were analyzed using SSR and RAPD markers. Based on a preliminary study of leaf morphological traits, the individuals separated into six groups. The studied half-sib oak families were also compared for allelic diversity, including group variations; genotypic structure; genetic diversity; and the degree of genetic subdivision and differentiation. The level of genetic variation and subdivision was lower in the hybrid families than in the families of the parental species. Genotypic analysis of the half-sibling offspring showed the asymmetric nature of interspecific hybridization processes of pedunculate and sessile oaks in mixed stands.

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Genetic diversity and differentiation among populations of the pedunculate oak (Quercus robur) at the eastern margin of its range based on a new set of 95 SNP loci

Journal of Forestry Research ◽

10.1007/s11676-020-01265-w ◽

2020 ◽

Author(s):

Bernd Degen ◽

Yulai Yanbaev ◽

Ruslan Ianbaev ◽

Svetlana Bakhtina ◽

Albina Tagirova

Keyword(s):

Genetic Diversity ◽

Quercus Robur ◽

Spatial Genetic Structure ◽

Allelic Diversity ◽

Genetic Distances ◽

Fixation Index ◽

Pedunculate Oak ◽

Migration Routes ◽

Volga River ◽

Eastern Margin

AbstractThe aim of this study is to determine the genetic variability and differentiation among populations of pedunculate oak (Quercus robur) at the eastern margin of the species distribution range. We applied new set of 95 geographically informative nuclear SNP (single nucleotide polymorphism) loci developed using Double Digest Restriction Site Associated DNA (ddRAD), a new generation DNA sequencing technology. The study area is located in the basins of the Volga River and its two main tributaries, Kama and Belaya. Despite the strong reduction and fragmentation of pedunculate oak forests over the last several centuries and comparatively small sizes of present stands, a relatively similar genetic diversity of seven populations was observed (on average, allelic diversity υа = 1.472–1.603, observed heterozygosity HO = 0.305–0.358, expected heterozygosity HE = 0.272–0.343). The genetic distances between populations vary between 0.102 and 0.170 (on average, d0 = 0.128). We have identified the absence of a spatial genetic structure and the existence of genetically peculiar populations in the territory studied. The genetic distances of Gregorius between populations vary between 0.102 and 0.170 (on average, d0 = 0.128). Genetic differentiation among populations was statistically significant with a mean of the fixation index FST of 0.075. Populations from the Privolzhye Upplands and from northern parts of the study area make the most contribution to overall inter-population differentiation (d0 = 0.137). No significant differences exist among the samples at lowlands along the Volga river (d0 = 0.110). It was concluded that the multiplicity and different ages of local populations and the complexity of migration routes of the pedunculate oak from Pleistocene refugia and secondary Holocene shelters can cause this phenomenon.

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Temporal dynamics of allelic diversity in isolated population of pedunculate oak Quercus robur L. (Fagaceae)

Russian Journal of Genetics ◽

10.1134/s1022795412010048 ◽

2012 ◽

Vol 48 (1) ◽

pp. 123-124

Author(s):

J. Buschbom ◽

Yu. A. Yanbaev ◽

B. Degen ◽

A. A. Gabitova

Keyword(s):

Quercus Robur ◽

Temporal Dynamics ◽

Allelic Diversity ◽

Pedunculate Oak ◽

Isolated Population

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Genetic Diversity and Differentiation of Pedunculate Oak (Quercus robur L.) Populations at the Southern Margin of Its Distribution Range—Implications for Conservation

Diversity ◽

10.3390/d13080371 ◽

2021 ◽

Vol 13 (8) ◽

pp. 371

Author(s):

Lazar Kesić ◽

Klára Cseke ◽

Saša Orlović ◽

Dejan B. Stojanović ◽

Saša Kostić ◽

...

Keyword(s):

Genetic Diversity ◽

Quercus Robur ◽

Allelic Richness ◽

Distribution Range ◽

Gene Pools ◽

Pedunculate Oak ◽

Rear Edge ◽

Southern Margin ◽

Genetic Diversity And Structure

Understanding intraspecific genetic variation is one of the principal requirements for the evaluation of tree species capacity to cope with intensive climatic changes, as well as designing long-term conservation programs. Herein, we evaluated the genetic diversity and genetic structure of seven pedunculate oak (Quercus robur L.) populations, located at the southern margin of its distribution range on the Balkan Peninsula (Serbia). The objective of the study was to propose future in situ conservation measures aimed at protection of pedunculate oak adaptive and neutral genetic diversity at the species rear-edge. Genetic diversity and structure were estimated using twelve highly polymorphic simple sequence repeat (SSR) markers. The mean expected heterozygosity (He) was 0.769, allelic richness (AR) 9.63, and private allelic richness (pAR) 0.79, indicating high genetic diversity in the studied populations. Genetic differentiation among the populations was low (Fst = 0.032). Structure analysis, the unweighted pair group method with arithmetic mean (UPGMA) showed the existence of two gene pools unrelated to the populations’ area of occurrence. Taking into consideration the results of the current study and previous conservation activities on the pedunculate oak in Serbia, as well as the importance of rear-edge populations in the long-term conservation of the species genetic diversity, we suggested establishing three additional gene conservation units for securing long-term sustainability of the species.

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Genetic Diversity of Pedunculate Oak (Quercus robur L.) in Clonal Seed Orchards in Croatia, Assessed by Nuclear and Chloroplast Microsatellites

South-east European forestry ◽

10.15177/seefor.18-09 ◽

2018 ◽

Vol 9 (1) ◽

Author(s):

Ida Katičić Bogdan ◽

Davorin Kajba ◽

Zlatko Šatović ◽

Silvio Schüler ◽

Saša Bogdan ◽

...

Keyword(s):

Genetic Diversity ◽

Quercus Robur ◽

Chloroplast Microsatellites ◽

Pedunculate Oak ◽

Seed Orchards

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Variability of wood extractives among Quercus robur and Quercus petraea trees from mixed stands and their relation to wood anatomy and leaf morphology

Canadian Journal of Forest Research ◽

10.1139/x98-066 ◽

1998 ◽

Vol 28 (7) ◽

pp. 994-1006 ◽

Cited By ~ 13

Author(s):

J R Mosedale ◽

F Feuillat ◽

R Baumes ◽

J -L Dupouey ◽

J -L Puech

Keyword(s):

Quercus Robur ◽

Leaf Morphology ◽

Wood Anatomy ◽

Chemical Constituents ◽

Morphological Characteristics ◽

Quercus Petraea ◽

Pedunculate Oak ◽

Species Trees ◽

Mixed Stands ◽

Cis And Trans

Heartwood ellagitannins and volatile extractives were studied by high-pressure liquid chromatography and gas chromatography - mass spectrometry after extraction of wood samples taken from 54 mature trees of pedunculate oak (Quercus robur L.) and sessile oak (Quercus petraea (Matt.) Liebl.) from 14 mixed stands located in the forest of Cîteaux (Bourgogne). These same trees had been previously subject to detailed study of leaf morphology and wood anatomy. Over 50 compounds, of diverse chemical types, were identified in the wood extracts and over 30 of these compounds were quantified. No species-specific extractives were identified, but the amounts of ellagitannins and a small number of volatile compounds, including cis- and trans-β-methyl-γ-octalactone isomers, were found to vary significantly between the two species. Multivariate analyses of these extractives allowed the classification of samples according to species, although less distinctly than similar analyses using leaf morphological characteristics. No strong intraspecific correlations between chemical constituents and anatomical or morphological characteristics were found that were common for both species. Nonetheless, sessile oaks displaying atypical wood anatomy were also less morphologically and chemically representative of their species. Trees possessing the lowest levels of cis- and trans-β-methyl-γ-octalactone are found to be those with a morphology the least distinct of either species.

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Norway maple (Acer platanoides) and pedunculate oak (Quercus robur) demonstrate different patterns of genetic variation within and among populations on the eastern border of distribution ranges

Journal of Forest Science ◽

10.17221/78/2021-jfs ◽

2021 ◽

Vol 67 (No. 11) ◽

pp. 522-532

Author(s):

Artur Akhmetov ◽

Ruslan Ianbaev ◽

Svetlana Boronnikova ◽

Yulai Yanbaev ◽

Aygul Gabitova ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Genetic Differentiation ◽

Population Genetic ◽

Quercus Robur ◽

Distribution Area ◽

Pedunculate Oak ◽

Acer Platanoides ◽

Norway Maple ◽

Key Species

Norway maple (Acer platanoides L.) is a key species of broadleaved forests whose population genetics is poorly studied using modern genetic tools. We used ISSR analysis to explore genetic diversity and differentiation among 10 Russian populations on the eastern margin of the species range of distribution, and to compare the revealed patterns with the results of our population genetic studies of pedunculate oak (Quercus robur L.). In the first set comparatively high heterozygosity and allelic diversity were found (expected heterozygosity HE = 0.160 ± 0.033, number of alleles na = 1.440 ± 0.080, effective number of alleles ne = 1.271 ± 0.062) in comparison with strongly fragmented and geographically isolated small maple stands of the second set (HE = 0.083 ± 0.011, na = 1.281 ± 0.031, ne = 1.136 ± 0.019). A relatively high genetic differentiation among populations was detected (the proportion of the inter-population component of total genetic variation, GST = 0.558 ± 0.038). In the Cis-Urals, local groups of populations that are confined to the northern, middle and southern parts of the Urals were identified. On the contrary, the current significant fragmentation of the pedunculate oak distribution area in the same study area did not lead to any noticeable genetic differentiation among the majority of populations, the values of the population genetic diversity were very similar in different parts of the Southern Urals.

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GENETIC DIVERSITY OF SNP LOCI IN POPULATIONS OF OAK IN THE SOUTHERN FOREST-STEPPE ZONE OF RUSSIA

Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology ◽

10.17072/1994-9952-2020-3-198-203 ◽

2020 ◽

pp. 198-203

Author(s):

B. Degen ◽

◽

Y. A. Yanbaev ◽

R. Y. Ianbaev ◽

S. Y. Bakhtina ◽

...

Keyword(s):

Genetic Diversity ◽

Allelic Diversity ◽

Steppe Zone ◽

Inbreeding Coefficient ◽

Oak Forests ◽

Forest Steppe ◽

Pedunculate Oak ◽

New Genera ◽

Sequencing Technology ◽

Hardy Weinberg Equilibrium

The level of genetic diversity of populations of pedunculate oak (Quercus robur L.) of the southern part of the forest-steppe zone of Russia was studied using 79 SNP loci, developed on the base of the new genera-tion DNA sequencing technology ddRAD. Despite the significant decrease of the area of oak forests in the agricultural period, populations from the Lipetsk, Orel and Penza regions maintain a comparatively high and relatively similar levels of genetic diversity (observed heterozygosity HO = 0.319–0.344, ex-pected heterozygosity HE = 0.317–0.342, allelic diversity υа = 1.537–1.561). The observed distribution of genotypes corresponds to the frequencies expected by the Hardy-Weinberg equilibrium, the inbreeding coefficient varies from F = ‒ 0.019 to = F = + 0.011. The genetic distance of Gregorius between the sam-ples Orel and Penza (d0 = 0.138), which are geographically the most distant from each other, were statisti-cally significant. The results obtained are discussed in regard to the dynamics of oak forests in the histori-cal past and to problems of conservation and rational use of the gene pool of local populations.

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