Chitosan–Fe3O4 nanoparticle enzymatic electrodes on paper as an efficient assay for glucose and uric acid detection in biological fluids

2020 ◽  
Vol 74 (8) ◽  
pp. 2675-2687
Author(s):  
Ahmad Manbohi ◽  
Seyyed Hamid Ahmadi
Keyword(s):  
Uric Acid ◽  
Biological Fluids ◽  
Fe3o4 Nanoparticle

Interaction of uranium(VI) with bioligands present in human biological fluids: the case study of urea and uric acid

2013 ◽  
Vol 101 (3) ◽  
pp. 139-148 ◽  
Author(s):  
Alfatih A. A. Osman ◽  
Gerhard Geipel ◽  
Gert Bernhard
Keyword(s):  
Uric Acid ◽  
Biological Fluids

Determination of Uric Acid in Biological Fluids by Ceria Nanoparticles Doped Reduced Graphene Oxide Nanocomposite Voltammetric Sensor

2021 ◽  
Author(s):  
Peihong Deng ◽  
Jinxia Feng ◽  
Jingyun Xiao ◽  
Yanping Wei ◽  
Jinsong Zuo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Uric Acid ◽  
Electrochemical Impedance ◽  
Kidney Diseases ◽  
Biological Fluids ◽  
Neurological Diseases ◽  
High Sensitivity ◽  
Ceria Nanoparticles ◽  
Structure And Property ◽  
Reduced Graphene

Abstract High levels of uric acid (UA) in the human body usually cause diabetes, hypertension and atherosclerosis, kidney diseases, and neurological diseases. Hence, it is important to develop sensitive methods for UA determination. In this paper, nanocomposite composed of ceria nanoparticles and reduced graphene was successfully modified on the surface of glassy carbon electrode (ceria NPs-rGO/GCE) by a simple electroreduction method. The morphology, structure and property of the ceria NPs-rGO/GCE was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The electrocatalytic activity of the ceria NPs-rGO/GCE for uric acid (UA) oxidation was studied in detail. The results showed that the ceria NPs-rGO/GCE exhibited excellent selectivity and high sensitivity for UA detection. In 0.05 M H2SO4 solution, a linear range of 0.02-20 M and a low detection limit of 8.0 nM of UA were obtained on the ceria NPs-rGO/GCE. This developed method was successfully applied for the detection of UA in human serum and urine samples, and its recoveries reached 95.8%-105.0%.

Highly sensitive colorimetric detection of glucose and uric acid in biological fluids using chitosan-modified paper microfluidic devices

The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4749-4756 ◽  
Author(s):  
Ellen F. M. Gabriel ◽  
Paulo T. Garcia ◽  
Thiago M. G. Cardoso ◽  
Flavio M. Lopes ◽  
Felipe T. Martins ◽  
...  
Keyword(s):  
Uric Acid ◽  
Biological Fluids ◽  
Colorimetric Detection ◽  
Microfluidic Devices ◽  
Analytical Performance ◽  
Highly Sensitive ◽  
Colorimetric Measurements ◽  
Paper Microfluidic ◽  
Enzymatic Bioassays

This paper describes the modification of microfluidic paper-based analytical devices (μPADs) with chitosan to improve the analytical performance of colorimetric measurements associated with enzymatic bioassays.

scholarly journals Mathematical modeling for bioprocess optimization of a protein drug, uricase, production by Aspergillus welwitschiae strain 1–4

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
S. A. Haroun ◽  
Eman M. El-Weshy ◽  
E. A. Metwally ◽  
A. A. Sherief
Keyword(s):  
Uric Acid ◽  
Yeast Extract ◽  
Incubation Time ◽  
Biological Fluids ◽  
Its Region ◽  
Clinical Analysis ◽  
Inoculum Size ◽  
Protein Drug ◽  
Statistical Screening ◽  
Coloring Agents

Abstract Microbial uricase is effective protein drug used to treat hyperuricemia and its complications, including chronic gout, also in prophylaxis and treatment of tumor lysis and organ transplants hyperuricemia. Uricase is commonly used as diagnostic reagent in clinical analysis for quantification of uric acid in blood and other biological fluids. Also, it can be used as an additive in formulations of hair coloring agents. A newly isolated strain, Aspergillus sp. 1–4, was able to produce extracellular uricase on a medium containing uric acid as inducer. Phylogenetic analysis based on ITS region sequence analysis and phenotypic characteristics showed that Aspergillus sp. strain 1–4 is closely related to Aspergillus welwitschiae and its nucleotide sequence was deposited in the GenBank database and assigned sequence accession number MG323529. Statistical screening using Plackett-Burman design with 20 runs was applied to screen fifteen factors for their significance on uricase production by Aspergillus welwitschiae. Results of statistical analysis indicated that incubation time has the most significant positive effect on uricase production followed by yeast extract and inoculum size with the highest effect values of 13.48, 5.26 and 4.75; respectively. The interaction effects and optimal levels of these factors were evaluated using central composite design. The maximum uricase production was achieved at incubation time (5 days), yeast extract (2 g/L) and inoculum size (4 mL/50 mL medium) are the optimum levels for maximum uricase production (60.03 U/mL). After optimization, uricase production increased by 3.02-folds as compared with that obtained from the unoptimized medium (19.87 U/mL).

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