scholarly journals Mathematical modeling for bioprocess optimization of a protein drug, uricase, production by Aspergillus welwitschiae strain 1–4

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
S. A. Haroun ◽  
Eman M. El-Weshy ◽  
E. A. Metwally ◽  
A. A. Sherief
Keyword(s):  
Uric Acid ◽  
Yeast Extract ◽  
Incubation Time ◽  
Biological Fluids ◽  
Its Region ◽  
Clinical Analysis ◽  
Inoculum Size ◽  
Protein Drug ◽  
Statistical Screening ◽  
Coloring Agents

Abstract Microbial uricase is effective protein drug used to treat hyperuricemia and its complications, including chronic gout, also in prophylaxis and treatment of tumor lysis and organ transplants hyperuricemia. Uricase is commonly used as diagnostic reagent in clinical analysis for quantification of uric acid in blood and other biological fluids. Also, it can be used as an additive in formulations of hair coloring agents. A newly isolated strain, Aspergillus sp. 1–4, was able to produce extracellular uricase on a medium containing uric acid as inducer. Phylogenetic analysis based on ITS region sequence analysis and phenotypic characteristics showed that Aspergillus sp. strain 1–4 is closely related to Aspergillus welwitschiae and its nucleotide sequence was deposited in the GenBank database and assigned sequence accession number MG323529. Statistical screening using Plackett-Burman design with 20 runs was applied to screen fifteen factors for their significance on uricase production by Aspergillus welwitschiae. Results of statistical analysis indicated that incubation time has the most significant positive effect on uricase production followed by yeast extract and inoculum size with the highest effect values of 13.48, 5.26 and 4.75; respectively. The interaction effects and optimal levels of these factors were evaluated using central composite design. The maximum uricase production was achieved at incubation time (5 days), yeast extract (2 g/L) and inoculum size (4 mL/50 mL medium) are the optimum levels for maximum uricase production (60.03 U/mL). After optimization, uricase production increased by 3.02-folds as compared with that obtained from the unoptimized medium (19.87 U/mL).

Interaction of uranium(VI) with bioligands present in human biological fluids: the case study of urea and uric acid

2013 ◽  
Vol 101 (3) ◽  
pp. 139-148 ◽  
Author(s):  
Alfatih A. A. Osman ◽  
Gerhard Geipel ◽  
Gert Bernhard
Keyword(s):  
Uric Acid ◽  
Biological Fluids

Determination of Uric Acid in Biological Fluids by Ceria Nanoparticles Doped Reduced Graphene Oxide Nanocomposite Voltammetric Sensor

2021 ◽  
Author(s):  
Peihong Deng ◽  
Jinxia Feng ◽  
Jingyun Xiao ◽  
Yanping Wei ◽  
Jinsong Zuo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Uric Acid ◽  
Electrochemical Impedance ◽  
Kidney Diseases ◽  
Biological Fluids ◽  
Neurological Diseases ◽  
High Sensitivity ◽  
Ceria Nanoparticles ◽  
Structure And Property ◽  
Reduced Graphene

Abstract High levels of uric acid (UA) in the human body usually cause diabetes, hypertension and atherosclerosis, kidney diseases, and neurological diseases. Hence, it is important to develop sensitive methods for UA determination. In this paper, nanocomposite composed of ceria nanoparticles and reduced graphene was successfully modified on the surface of glassy carbon electrode (ceria NPs-rGO/GCE) by a simple electroreduction method. The morphology, structure and property of the ceria NPs-rGO/GCE was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The electrocatalytic activity of the ceria NPs-rGO/GCE for uric acid (UA) oxidation was studied in detail. The results showed that the ceria NPs-rGO/GCE exhibited excellent selectivity and high sensitivity for UA detection. In 0.05 M H2SO4 solution, a linear range of 0.02-20 M and a low detection limit of 8.0 nM of UA were obtained on the ceria NPs-rGO/GCE. This developed method was successfully applied for the detection of UA in human serum and urine samples, and its recoveries reached 95.8%-105.0%.

Construction of Hyperuricemia Model Rats with Yeast Extract Combined with Oteracil Potassium

2020 ◽  
Author(s):  
Dilidaer Xilifu ◽  
Alimu Kateer ◽  
Nijiati Rehemu ◽  
Zhao-yong Li ◽  
jie Jiang ◽  
...  
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Yeast Extract ◽  
Intraperitoneal Injection ◽  
Rat Kidney ◽  
Optimal Dose ◽  
Serum Levels ◽  
Male Rats ◽  
Control Group ◽  
Optimal Dosage

Abstract Background: Hyperuricemia is the most important risk factor for gout, hypertension, coronary artery disease and other cardiovascular diseases. The incidence of hyperuricemia gradually increased in recent years and it is very necessary to explore the medications of the prevention and treatment of hyperuricemia using hyperuricemia animal models. Objective: The objective of present study is to explore the optimal dose of yeast extract and oteracil potassium in the establishment of hyperuricemia rat model. Method: Sixty-four male rats were randomly divided into 8 experimental groups. Rats were treated with yeast extract by intraperitoneal injection or yeast extract by intraperitoneal injection combined with various doses of oteracil potassium by intragastric feeding or intraperitoneal injection for 28 days. The serum uric acid, urea nitrogen and creatinine levels of different groups were measured at 0th day, 7th day, 14th day, 21th day and 28th day. Results: The serum levels of uric acid in the groups of intraperitoneal injection with yeast extract alone, yeast extract by intraperitoneal injection combined with 50-200 mg/kg oteracil potassium by intragastric feeding and yeast extract by intraperitoneal injection combined with 50-100 mg/kg oteracil potassium by intraperitoneal injection were higher than that in the control group. But we found no significant effect on rat kidney, heart or artery in the above groups. In the group of yeast extract by intraperitoneal injection combined with 200 mg/kg oteracil potassium by intraperitoneal injection, we observed the significantly high level of serum uric acid and morphological and pathological changes in rat kidney, heart and artery. Conclusion: In the present study, we found that continuously treated with yeast extract combined with oteracil potassium is an effective method to establish rat hyperuricemia model. Intraperitoneal injection of yeast extract combined with 200 mg/kg oteracil potassium is an optimal dosage for the construction of a persistent and stable hyperuricemia animal model.

Solid-phase radioimmunoassay of human complement fragment C5a.

1988 ◽  
Vol 34 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
S Chang ◽  
A Leo-Mensah ◽  
J Campbell ◽  
M Stastny ◽  
R A Patrick
Keyword(s):  
Incubation Time ◽  
Solid Phase ◽  
Biological Fluids ◽  
Reference Method ◽  
Human Complement ◽  
Analytical Recovery ◽  
Solid Phase Radioimmunoassay

Abstract In this competitive RIA for determining concentrations of human C5a in biological fluids and in buffers, labeled C5a and sample are allowed to compete for binding to a limited amount of goat antibody to human C5a in solution. Free and bound tracer are then separated by a second antibody (rabbit anti-goat IgG) immobilized on paramagnetic particles. Total incubation time for this assay is 70 min. Sensitivity, precision, and analytical recovery of this assay compare well with those of a reference method.

Effect of Four Presumptive Coliform Test Media, Incubation Time and Product Inoculum Size on Recovery of Coliforms from Dairy Products

1985 ◽  
Vol 48 (5) ◽  
pp. 388-392 ◽  
Author(s):  
B. C. COOKE ◽  
M. A. JORGENSEN ◽  
A. B. MacDONALD
Keyword(s):  
Bile Salt ◽  
Media Studies ◽  
Incubation Time ◽  
Dairy Products ◽  
Limited Range ◽  
Inoculum Size ◽  
Media Type ◽  
Defined Media ◽  
Media Comparison ◽  
Better Than

The need to consider the interrelationship of relevant procedural parameters in media comparison studies has been shown. In this study, the influence of media-type on recovery of coliforms from dairy products was found to be of less importance than the effects of incubation time and inoculum size. Statistically significant media-type effects were found, however, and these contrasted with the observations of other workers in that bile-salt-based media performed better than chemically-defined media. These findings indicate that media studies too specifically designed may yield conclusions with limited range of application.

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