Mitochondrial RNA editing of F0-ATPase subunit 9 gene (atp9) transcripts of Yunnan purple rice cytoplasmic male sterile line and its maintainer line

Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. Our study provides a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR/Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.

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Comparative expression profiling of miRNAs between the cytoplasmic male sterile line MeixiangA and its maintainer line MeixiangB during rice anther development

Planta ◽

10.1007/s00425-014-2167-2 ◽

2014 ◽

Vol 241 (1) ◽

pp. 109-123 ◽

Cited By ~ 40

Author(s):

Junjie Yan ◽

Hongyuan Zhang ◽

Yinzheng Zheng ◽

Yi Ding

Keyword(s):

Expression Profiling ◽

Anther Development ◽

Male Sterile ◽

Male Sterile Line ◽

Maintainer Line ◽

Cytoplasmic Male Sterile

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Comparison on the Biochemical Characteristics between Alfalfa Sterile Line and Maintainer Line

E3S Web of Conferences ◽

10.1051/e3sconf/202020302002 ◽

2020 ◽

Vol 203 ◽

pp. 02002

Author(s):

YAN Dong ◽

XU Xiaobo ◽

GUO Qiang ◽

XU Bo

Keyword(s):

Antioxidant Enzyme ◽

Soluble Protein ◽

Soluble Sugar ◽

Pollen Grain ◽

Abnormal Development ◽

Free Proline ◽

Male Sterile ◽

Male Sterile Line ◽

Maintainer Line ◽

Cytoplasmic Male Sterile

To study on the morphological character of cytology and physiological and biochemical mechanism of alfalfa cytoplasmic male sterile line, this paper took sterile line MS-GN1A and maintainer line MS-GN1B as raw material, separately conducted microscopic observation to anther in different development stage and determined the content of soluble sugar, starch, soluble protein, free proline and malondialdehyde, and the activity of antioxidant enzyme (catalase, peroxidase and superoxide dismutase). The results showed that: 1. Compared with maintainer line, the pollen grain of alfalfa cytoplasmic male sterile line presented shriveled appearance, smaller size with deletion and few; during the tetrad period of pollen, tapetum cell abnormally grew; callose and epidermis didn’t disintegrate and couldn’t release pollen grain; pollen abortion of alfalfa cytoplasmic sterile line was related to the abnormal development of connective vascular bundle. 2. With the growth and development of bud and the development and maturity of pollen, the content of soluble sugar, starch, soluble protein, and free proline showed deficient phenomenon in each development period of bud of sterile line MS-GN1A; as peroxidation index of membranous substance, the activity of malondialdehyde and antioxidant enzyme was higher than it of homotype maintainer line MS-GN1B.

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