Comparative expression profiling of miRNAs between the cytoplasmic male sterile line MeixiangA and its maintainer line MeixiangB during rice anther development

Planta ◽  
2014 ◽  
Vol 241 (1) ◽  
pp. 109-123 ◽  
Author(s):  
Junjie Yan ◽  
Hongyuan Zhang ◽  
Yinzheng Zheng ◽  
Yi Ding
Keyword(s):  
Expression Profiling ◽  
Anther Development ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile

scholarly journals Expression profiling of immature florets of IR58025A, a wild-abortive cytoplasmic male sterile line of rice and its cognate, isonuclear maintainer line, IR58025B

3 Biotech ◽  
2019 ◽  
Vol 9 (7) ◽  
Author(s):  
K. Pranathi ◽  
M. B. Kalyani ◽  
B. C. Viraktamath ◽  
S. M. Balachandran ◽  
S. K. Hajira ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile

A comparative light and electron microscopic study of microsporogenesis in male-fertile and cytoplasmic male-sterile pepper (Capsicum annuum)

1974 ◽  
Vol 52 (3) ◽  
pp. 435-441 ◽  
Author(s):  
Harry T. Horner Jr. ◽  
Milton A. Rogers
Keyword(s):  
Anther Development ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Electron Microscopic ◽  
Tetrad Stage ◽  
Cytoplasmic Male Sterile ◽  
Tapetal Cells ◽  
Fertile Line ◽  
Pollen Stage ◽  
Further Development

In the male-fertile line of pepper, microsporogenesis and pollen development are normal. During meiosis, the meiocytes become encased in callose and a locular cavity forms. A rudimentary pollen wall, preceded by primexine deposition, is formed at the tetrad stage around the microspores before their release from the callose. The tapetum remains peripheral in the locule until the vacuolate pollen stage when it disappears. The sporogenous cells of the cytoplasmic male-sterile line complete meiosis, and the callose-encased microspores also deposit a primexine. Further development of the microspores is arrested. Before and during meiosis the tapetal cells become highly vacuolate and remain appressed to the meiocytes; a locular cavity is not formed. After primexine deposition, the tetrads of microspores, which are still encased in callose, seem to collapse as they are encroached upon by the vacuolate tapetum. After abortion of the microspores the outer tapetal layer degenerates, followed by the inner tapetal layer. The aborted mass late in anther development consists of crushed microspore tetrads, primary walls of the sporogenous cells and tapetum, callose, and the collapsed tapetum. The manner of abortion in pepper is compared with previously described mechanisms.

scholarly journals Breeding of Indica glutinous cytoplasmic male sterile line WX209A via CRISPR/Cas9 mediated genomic editing

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 93-100 ◽  
Author(s):  
Xin Wang ◽  
Yue Han ◽  
Xuan Feng ◽  
Yun-Zhen Li ◽  
Bao-Xiang Qin ◽  
...  
Keyword(s):  
Amylose Content ◽  
Rice Variety ◽  
High Yield ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile ◽  
Glutinous Rice ◽  
Short Period ◽  
Cms Line

Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. Our study provides a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR/Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.  

scholarly journals Comparison on the Biochemical Characteristics between Alfalfa Sterile Line and Maintainer Line

2020 ◽  
Vol 203 ◽  
pp. 02002
Author(s):  
YAN Dong ◽  
XU Xiaobo ◽  
GUO Qiang ◽  
XU Bo
Keyword(s):  
Antioxidant Enzyme ◽  
Soluble Protein ◽  
Soluble Sugar ◽  
Pollen Grain ◽  
Abnormal Development ◽  
Free Proline ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile

To study on the morphological character of cytology and physiological and biochemical mechanism of alfalfa cytoplasmic male sterile line, this paper took sterile line MS-GN1A and maintainer line MS-GN1B as raw material, separately conducted microscopic observation to anther in different development stage and determined the content of soluble sugar, starch, soluble protein, free proline and malondialdehyde, and the activity of antioxidant enzyme (catalase, peroxidase and superoxide dismutase). The results showed that: 1. Compared with maintainer line, the pollen grain of alfalfa cytoplasmic male sterile line presented shriveled appearance, smaller size with deletion and few; during the tetrad period of pollen, tapetum cell abnormally grew; callose and epidermis didn’t disintegrate and couldn’t release pollen grain; pollen abortion of alfalfa cytoplasmic sterile line was related to the abnormal development of connective vascular bundle. 2. With the growth and development of bud and the development and maturity of pollen, the content of soluble sugar, starch, soluble protein, and free proline showed deficient phenomenon in each development period of bud of sterile line MS-GN1A; as peroxidation index of membranous substance, the activity of malondialdehyde and antioxidant enzyme was higher than it of homotype maintainer line MS-GN1B.

scholarly journals Combined Transcriptome and Proteome Analysis of Anthers of AL-type Cytoplasmic Male Sterile Line and Its Maintainer Line Reveals New Insights into Mechanism of Male Sterility in Common Wheat

2021 ◽  
Vol 12 ◽  
Author(s):  
Miaomiao Hao ◽  
Wenlong Yang ◽  
Tingdong Li ◽  
Muhammad Shoaib ◽  
Jiazhu Sun ◽  
...  
Keyword(s):  
Male Sterility ◽  
Proteome Analysis ◽  
Acid Synthesis ◽  
Anther Development ◽  
Ros Scavenging ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
New Perspective

Cytoplasmic male sterility (CMS) plays an essential role in hybrid seeds production. In wheat, orf279 was reported as a CMS gene of AL-type male sterile line (AL18A), but its sterility mechanism is still unclear. Therefore, transcriptomic and proteomic analyses of the anthers of AL18A and its maintainer line (AL18B) were performed to interpret the sterility mechanism. Results showed that the electron transport chain and ROS scavenging enzyme expression levels changed in the early stages of the anther development. Biological processes, i.e., fatty acid synthesis, lipid transport, and polysaccharide metabolism, were abnormal, resulting in pollen abortion in AL18A. In addition, we identified several critical regulatory genes related to anther development through combined analysis of transcriptome and proteome. Most of the genes were enzymes or transcription factors, and 63 were partially homologous to the reported genic male sterile (GMS) genes. This study provides a new perspective of the sterility mechanism of AL18A and lays a foundation to study the functional genes of anther development.

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