scholarly journals RNA Editing Responses to Oxidative Stress between a Wild Abortive Type Male-Sterile Line and Its Maintainer Line

2017 ◽  
Vol 8 ◽  
Author(s):  
Jie Xiong ◽  
Tao Tao ◽  
Zhi Luo ◽  
Shuaigang Yan ◽  
Yi Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rna Editing ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line

scholarly journals Differential RNA Editing of Mitochondrial Genes in WA-Cytoplasmic Based Male Sterile Line Pusa 6A, and Its Maintainer and Restorer Lines

Rice Science ◽  
2019 ◽  
Vol 26 (5) ◽  
pp. 282-289
Author(s):  
Umakanta Ngangkham ◽  
Swarup Kumar Parida ◽  
Ashok Kumar Singh ◽  
Trilochan Mohapatra
Keyword(s):  
Rna Editing ◽  
Mitochondrial Genes ◽  
Male Sterile ◽  
Male Sterile Line

scholarly journals Breeding of Indica glutinous cytoplasmic male sterile line WX209A via CRISPR/Cas9 mediated genomic editing

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 93-100 ◽  
Author(s):  
Xin Wang ◽  
Yue Han ◽  
Xuan Feng ◽  
Yun-Zhen Li ◽  
Bao-Xiang Qin ◽  
...  
Keyword(s):  
Amylose Content ◽  
Rice Variety ◽  
High Yield ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile ◽  
Glutinous Rice ◽  
Short Period ◽  
Cms Line

Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. Our study provides a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR/Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.  

scholarly journals Comparison on the Biochemical Characteristics between Alfalfa Sterile Line and Maintainer Line

2020 ◽  
Vol 203 ◽  
pp. 02002
Author(s):  
YAN Dong ◽  
XU Xiaobo ◽  
GUO Qiang ◽  
XU Bo
Keyword(s):  
Antioxidant Enzyme ◽  
Soluble Protein ◽  
Soluble Sugar ◽  
Pollen Grain ◽  
Abnormal Development ◽  
Free Proline ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile

To study on the morphological character of cytology and physiological and biochemical mechanism of alfalfa cytoplasmic male sterile line, this paper took sterile line MS-GN1A and maintainer line MS-GN1B as raw material, separately conducted microscopic observation to anther in different development stage and determined the content of soluble sugar, starch, soluble protein, free proline and malondialdehyde, and the activity of antioxidant enzyme (catalase, peroxidase and superoxide dismutase). The results showed that: 1. Compared with maintainer line, the pollen grain of alfalfa cytoplasmic male sterile line presented shriveled appearance, smaller size with deletion and few; during the tetrad period of pollen, tapetum cell abnormally grew; callose and epidermis didn’t disintegrate and couldn’t release pollen grain; pollen abortion of alfalfa cytoplasmic sterile line was related to the abnormal development of connective vascular bundle. 2. With the growth and development of bud and the development and maturity of pollen, the content of soluble sugar, starch, soluble protein, and free proline showed deficient phenomenon in each development period of bud of sterile line MS-GN1A; as peroxidation index of membranous substance, the activity of malondialdehyde and antioxidant enzyme was higher than it of homotype maintainer line MS-GN1B.

scholarly journals USE OF MALE STERILITY AND SYNTHESIS OF MAINTAINER LINE FOR HYBRID SEED PRODUCTION IN ONION (Allium cepa L.)

2016 ◽  
Vol 29 (1) ◽  
pp. 31-38
Author(s):  
G. M. Mohsin ◽  
Farruk Ahmed ◽  
M. S. Rahman ◽  
M. S. Islam
Keyword(s):  
Male Sterility ◽  
F1 Hybrids ◽  
Recurrent Parent ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Local Cultivar ◽  
Allium Cepa L ◽  
Hybrid Variety

Field experiment was carried out and one cytoplasmic genetic male sterile line (Smsms) and two local lines were identified as maintainer lines (Fmsms). These two crossed materials namely 3(Shallot*Local) and 4 (Shallot *Local) produced 100 per cent male sterile progeny in full sib and backcross generations. The Shallot * market cultivar produced both male fertile and male sterile segregating progenies. It indicated that the market material is probably determined by dominant and recessive independently acting genes which genetically impure lines. All other crossed materials produced 100 per cent male fertile progeny upon crossing with shallot. So, in backcross generations, the male sterile plants were more when local was used as recurrent parent. Thus, the local cultivar can be used as maintainer line for “Shallot”. The performance of 904 F1 and 905 F1 hybrids over check and better parent was found to be preferably better using CMS system. So, considering the genetics and stability of the male sterility mechanism, further studies are needed towards hybrid variety development in Bangladesh.

scholarly journals RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A

2019 ◽  
Vol 52 (1) ◽  
Author(s):  
Xiangjun Kong ◽  
Dongmei Liu ◽  
Jie Zheng ◽  
Aziz Khan ◽  
Bin Li ◽  
...  
Keyword(s):  
Rna Editing ◽  
Atp Synthase ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Cytoplasmic Male Sterile
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