Aluminum elicits tropane alkaloid production and antioxidant system activity in micropropagated Datura innoxia plantlets

In non-rooted shoots regenerated from calluses induced with nine different cyokinin–auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokinin–auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 µM + naphthaleneacetic acid (NAA) 1 µM and BA 10 µM + indole-3-butyric acid (IBA) 0.1 µM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 × 10 –4 and 6.5 × 10 –4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokinin–auxin combinations. No tropane alkaloids were detected in these shoots.

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Tropane alkaloid production inDatura stramonium root cultures

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/bf02823021 ◽

1992 ◽

Vol 28 (2) ◽

pp. 67-72 ◽

Cited By ~ 16

Author(s):

Ignacio E. Maldonado-Mendoza ◽

Terasa R. Ayora-Talavera ◽

Victor M. Loyola-Vargas

Keyword(s):

Tropane Alkaloid ◽

Root Cultures ◽

Alkaloid Production

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Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children’s Saliva

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/3695683 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Heitor Ceolin Araujo ◽

Ana Cláudia Melo Stevanato Nakamune ◽

Wilson Galhego Garcia ◽

Juliano Pelim Pessan ◽

Cristina Antoniali

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Oxidative Damage ◽

Antioxidant System ◽

Oxidative Stress Biomarkers ◽

System Activity ◽

Enzymatic Antioxidant ◽

Stress Biomarkers ◽

Salivary Biomarkers ◽

Carious Lesions

Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage.

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