Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides

2001 ◽  
Vol 49 (4) ◽  
pp. 443 ◽  
Author(s):  
Nurussaba Khanam ◽  
Cheang Khoo ◽  
Abdul G. Khan
Keyword(s):  
Secondary Xylem ◽  
Stem Elongation ◽  
Tropane Alkaloids ◽  
Dry Weight ◽  
Tropane Alkaloid ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Alkaloid Production ◽  
Cell Arrangement ◽  
Induction Stage

In non-rooted shoots regenerated from calluses induced with nine different cyokinin–auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokinin–auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 µM + naphthaleneacetic acid (NAA) 1 µM and BA 10 µM + indole-3-butyric acid (IBA) 0.1 µM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 × 10 –4 and 6.5 × 10 –4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokinin–auxin combinations. No tropane alkaloids were detected in these shoots.

Function of coprophagous beetles (Coleoptera: Scarabaeidae, Geotrupidae, Hydrophilidae) in cattle pastures inferred from pitfall trapping data

2010 ◽  
Vol 27 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Martin Šlachta ◽  
Jan Frelich ◽  
Tomáš Tonka
Keyword(s):  
Seasonal Variation ◽  
Spatial Variation ◽  
Dry Weight ◽  
Seasonal Distribution ◽  
The Other ◽  
Pitfall Trapping ◽  
Other Hand ◽  
Nested Anova ◽  
Species Groups ◽  
Dung Decomposition

Function of coprophagous beetles (Coleoptera: Scarabaeidae, Geotrupidae, Hydrophilidae) in cattle pastures inferred from pitfall trapping dataAn analysis of data on the dry weight biomass of coprophagous beetles in standardized dung (4.5 l) was conducted in order to characterize the spatial and the seasonal distribution of the beetles' biomass in cattle pastures and to elucidate their function in dung decomposition. Nested Anova with factors of farm, site (nested in farm), seasonal period and year was used to evaluate the effect of these factors on the biomass of four functional species groups: the dung dwellers ofScarabaeidae(subfamilyAphodiinae), the dung dwellers ofHydrophilidae, the small tunnellers ofScarabaeidae(subfamilyCoprinae) and the large tunnellers ofGeotrupidae. The spatial variation of biomass (between the sites and the farms) was insignificant (P>0.05) in the two dung-dweller groups and in the large-tunnellers group. On the other hand, a significant (P<0.05) seasonal variation of biomass was found in all but the large tunneller group. In dung dwellers, the spring biomass was formed mainly by two species,Aphodius prodromusandA. sphacelatus. In summer, most of the biomass was accounted for bySphaeridium lunatum, S. scarabaeoidesandA. rufipes. In the two tunneller groups,Onthophagus fracticornis, Geotrupes stercorariusandG. spinigerformed a majority of the biomass in dung.

scholarly journals Innovative Ultrasound-Assisted Approaches towards Reduction of Heavy Metals and Iodine in Macroalgal Biomass

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 649
Author(s):  
Estefanía Noriega-Fernández ◽  
Izumi Sone ◽  
Leire Astráin-Redín ◽  
Leena Prabhu ◽  
Morten Sivertsvik ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Dry Weight ◽  
The Other ◽  
Laminaria Hyperborea ◽  
Other Hand ◽  
Macroalgal Biomass ◽  
Ultrasound Assisted ◽  
I 31

The aim of this work was to evaluate the potential of ultrasound (US), alone or in combination with mild heating and/or EDTA towards reduction of As, Cd, I, and Hg content of Laminaria hyperborea. Concentrations of As, Cd, I, and Hg of 56.29, 0.596, 7340, and <0.01 mg kg−1 of dry weight, respectively, were found in L. hyperborea blades. Treatment with US at 50 °C increased approx. 2-fold the amount of As released, although did not affect significantly the content of Cd or I, as compared to control (no US) samples. Reducing the temperature to 8 °C significantly decreased the effect of US, but heating at 80 °C did not cause a significant effect as compared to treatments at 50 °C. On the other hand, treatment with 0.1 N EDTA at 50 °C enhanced the percentage of Cd released by approximately 7-fold, regardless of sonication. In the present work, the combination of US and EDTA at 50 °C for 5 min led to a significant reduction of the As (32%), Cd (52%) and I (31%) content in L. hyperborea, thus improving the product’s safety for consumers.

Soybean (Glycine max) Cultivar Response to Metribuzin in Solution Culture

Weed Science ◽  
1979 ◽  
Vol 27 (3) ◽  
pp. 278-279 ◽  
Author(s):  
W. S. Hardcastle
Keyword(s):  
Glycine Max ◽  
Dry Weight ◽  
Solution Culture ◽  
Hydroponic Culture ◽  
The Other ◽  
Top Dry Weight

Twenty-eight commercial soybean [Glycine max(L.) Merr.] cultivars of maturity classes V through VIII were evaluated for differences in response to metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] 0.125 ppm w/w in hydroponic culture. Top dry weight (TDW) of treated ‘FFR 666’ soybeans equaled that of the cultivar check and five other cultivars were not significantly different (P = 5%). ‘Semmes' was most sensitive to the herbicide with TDW 40% of cultivar check. ‘Tracy’ and ‘Coker 156’ were not significantly different (P = 5%) from Semmes. The other cultivars tested were intermediate in response to metribuzin.

Effect of nitrogen fertilizer on growth and yield of semi-dwarf and tall varieties of winter wheat

1978 ◽  
Vol 91 (1) ◽  
pp. 31-45 ◽  
Author(s):  
I. Pearman ◽  
S. M. Thomas ◽  
G. N. Thorne
Keyword(s):  
Winter Wheat ◽  
Grain Yield ◽  
Leaf Area ◽  
Dry Weight ◽  
The Other ◽  
Growth And Yield ◽  
Shoot Dry Weight ◽  
Phosphorus And Potassium ◽  
Main Effects ◽  
Dwarf Variety

SummaryEight amounts of nitrogen ranging from 0 to 210 kg N/ha were applied to two tall and one semi-dwarf variety of winter wheat in the spring of 1975 and 1976. The tall varieties were Cappelle-Desprez and Maris Huntsman; the semi-dwarf variety was Maris Fundin in 1975 and Hobbit in 1976. Interactions between varieties and nitrogen were few and small compared with the main effects. All varieties produced their maximum grain yields with 180 kg N/ha. The yield of the semi-dwarf varieties, but not the others, decreased slightly with more nitrogen.Cappelle-Desprez yielded less grain than the other varieties in both years. In 1975 the yields of Maris Fundin and Maris Huntsman were similar and in 1976 Hobbit yielded more than Maris Huntsman. The varieties had similar numbers of ears at maturity and similar patterns of tillering. The semi-dwarf varieties had most grains per spikelet, and hence grains per ear, and Cappelle-Desprez had least. The semi-dwarf varieties had the smallest grains. The semi-dwarf varieties had less straw than the other varieties and hence the largest ratios of grain to total above-ground dry weight. The decrease in dry weight of stem and leaves between anthesis and maturity was similar for all varieties. In 1975 the efficiency of the top two leaves plus top internode in producing grain was the same for all varieties, but in 1976 Hobbit was more efficient than the other two. There were some small differences between varieties in nutrient uptake that were not related to differences in growth. Maris Fundin tended to have a greater phosphorus and potassium content than the tall varieties. Hobbit contained slightly less nitrogen than the tall varieties at maturity, and had a smaller concentration of nitrogen in the grain.Applying 210 kg N/ha doubled grain yield in 1975. Applying nitrogen resulted in a largeincrease in number of ears and a small increase in number of grains per ear due to the development of more fertile spikelets per ear. Nitrogen decreased dry weight per grain, especially of the semi-dwarf varieties. With extra nitrogen, straw dry weight at maturity, shoot dry weight atanthesis and leaf area were all increased relatively more than grain yield, and stems lost moredry weight between anthesis and maturity than without nitrogen. The year 1976 was exceptionallydry and nitrogen had only small effects in that it affected neither straw dry weight nor numberof ears but slightly increased grain yield by increasing the number of spikelets and number of grains per spikelet. It also increased leaf area proportionately to grain yield. In 1975 nitrogen increased evaporation of water from the crop before anthesis but decreased it after anthesis, even though it continued to increase the extraction of water from below 90 cm.

scholarly journals Comparison of methods for obtaining doubled haploids of carrot

2017 ◽  
Vol 86 (2) ◽  
Author(s):  
Waldemar Kiszczak ◽  
Urszula Kowalska ◽  
Agata Kapuścińska ◽  
Maria Burian ◽  
Krystyna Górecka
Keyword(s):  
Plant Regeneration ◽  
Doubled Haploid ◽  
Aspartate Aminotransferase ◽  
Doubled Haploids ◽  
The Other ◽  
Plant Production ◽  
Comparison Of Methods ◽  
Anther Cultures ◽  
Induction Stage ◽  
Main Factor

Doubled haploid lines of carrot can be obtained through androgenesis in anther cultures and in isolated microspore cultures. The two methods were compared using three carrot cultivars (‘Kazan F1’, ‘Feria F1’, and ‘Narbonne F1’) at the androgenesis induction stage, during plant regeneration from embryos, and during acclimatization of androgenetic plants as well as their characterization. It was found that cultivar was the main factor affecting the efficiency at each stage of plant production in both anther and isolated microspore cultures. The efficiency of androgenesis in anther cultures of ‘Feria F1’ was considerably higher in comparison with isolated microspore cultures, and more plants were obtained from the embryos of androgenesis-cultured plants. In ‘Kazan F1’ and ‘Narbonne F1’, more acclimatized androgenetic plants were produced from anther cultures. Ploidy assessment of acclimatized plants of ‘Narbonne F1’ showed that the majority of the plants in the population derived from anther cultures had a doubled chromosome (DH) set. On the other hand, the majority of plants obtained from isolated microspore cultures were haploids. When assessing homozygosity, it was found among plants obtained in anther cultures that the percentage of homozygotes for phosphoglucose isomerase (PGI) and aspartate aminotransferase (AAT) depended on the cultivar. In contrast, the majority of plants derived from isolated microspore cultures were homozygous regardless of cultivar.

scholarly journals Efecto de la inoculación de Azospirillum spp. sobre el contenido nutrimental de moringa (Moringa oleifera Lam.)

2020 ◽  
Vol 38 (1) ◽  
pp. 29
Author(s):  
Mario Alejandro Hernández-Chontal ◽  
Dinora Vazquez-Luna ◽  
Ariadna Linares-Gabriel ◽  
Gloria Esperanza De Dios-León ◽  
Armando Guerrero-Peña ◽  
...  
Keyword(s):  
Crude Protein ◽  
Moringa Oleifera ◽  
Calcium Content ◽  
Dry Weight ◽  
The Other ◽  
Nutritional Content ◽  
Inoculum Concentration ◽  
Nutrient Contents ◽  
Animal Consumption ◽  
Moringa Oleifera Lam

Moringa is used for human and animal consumption due to its high content of proteins, vitamins and minerals. The present study analyzed and evaluated the effectiveness of Azospirillum spp. over the nutritional content of moringa (Moringa oleifera Lam.) under nursery conditions. The experimental design consisted of four treatments with six repetitions in complete blocks at random, which consisted of the inoculation at 0.5, 1 and 1.5 mL of suspension with 9.8 × 105 CFU mL-1 of Azospirillum spp. The study variables: biomass, protein, macro (P, K. Ca and Mg) and micronutriments (Na, Fe, Cu and Mn) in leaves and stems were determined 70 days after sowing. The analysis of variance showed signif icant statistical differences between treatments only for the variables: potassium content in leaves and calcium content, iron and sodium in stem. According to the comparison of means (P ≤ 0.05) the best treatments were those that received 0.5 and 1 mL of suspension. The inoculation of Azospirillum, failed to increase the dry weight and protein contents; however, the highest values (30.2% crude protein) were associated with its application. The absence of significant effects in the other variables evaluated is associated with inoculum concentration, however, higher values are shown in the nutrient contents in relation to results reported in other research.

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

Tropane Alkaloid Production by the Establishment of Hairy Root Cultures of Brugmansia candida and Elicitation

2020 ◽  
pp. 123-132
Author(s):  
Alejandra B. Cardillo ◽  
María Perassolo ◽  
Juan M. Minoia ◽  
Julián Rodríguez Talou ◽  
Ana M. Giulietti
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Brugmansia Candida

scholarly journals Assessing Quantitative Criteria for Characterization of Quality Categories for Grafted Watermelon Seedlings

Horticulturae ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 16 ◽  
Author(s):  
Filippos Bantis ◽  
Athanasios Koukounaras ◽  
Anastasios Siomos ◽  
Georgios Menexes ◽  
Christodoulos Dangitsis ◽  
...  
Keyword(s):  
Dry Weight ◽  
The Other ◽  
Time Intervals ◽  
Important Species ◽  
Shoot Dry Weight ◽  
Shoot Ratio ◽  
Quality Categories ◽  
Critical Limits ◽  
Experienced Personnel

Vegetable grafting is a practice employed worldwide since it helps prevent biotic and abiotic disorders, and watermelon is one of the most important species grafted. The objective of this study was to set critical limits for the characterization of quality categories for grafted watermelon seedlings. Specifically, watermelon (scion) seedlings were grafted onto squash (rootstock) seedlings, moved into a healing chamber for 7 days, and then transferred into a greenhouse for seven more days. At 7 and 14 days after grafting, experienced personnel assessed grafted seedling quality by categorizing them. The categories derived were Optimum and Acceptable for both time intervals, plus Not acceptable at 14 days after grafting. Optimum seedlings showed greater leaf area, and shoot and root fresh and dry weights at both time intervals. Moreover, they had greater stem diameter, root-to-shoot ratio, shoot dry weight-to-length ratio and Dickson’s quality index compared to the other category at 14 days after grafting. Therefore, Optimum seedlings would likely develop into marketable plants of high quality, with better establishment in the field. Not acceptable seedlings showed considerably inferior development, while Acceptable seedlings were between the other categories, but were still marketable.

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