Kinetic model of asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester using Saccharomyces cerevisiae CGMCC No.2266

2010 ◽  
Vol 27 (6) ◽  
pp. 1841-1846 ◽  
Author(s):  
Xiaoyan Chen ◽  
Zhimin Ou ◽  
Guoqing Ying
2012 ◽  
Vol 560-561 ◽  
pp. 273-278
Author(s):  
Zhi Min Ou ◽  
Ying Kang Nan

An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid ethyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 20 g/L, cell concentration 140 g/L, reaction time 36 h, temperature 30 0C. Conversion and enantiometric excess of (R)-(-)-mandelic acid ethyl ester reached 99.8 % and 100%.


ChemInform ◽  
2010 ◽  
Vol 27 (41) ◽  
pp. no-no
Author(s):  
A. CHADHA ◽  
M. MANOHAR ◽  
T. SOUNDARARAJAN ◽  
T. S. LOKESWARI

Fermentation ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 66
Author(s):  
Braschi ◽  
Ricci ◽  
Grazia ◽  
Versari ◽  
Patrignani ◽  
...  

: The production of volatile compounds has become one of the major technological features for yeast selection. In fact, although the aromatic profile of the wine is the sum of varietal-, pre-, post-, and fermentative-aroma compound, yeasts affect the quality of the grape from maturation throughout fermentation, metabolizing sugars and other components into alcohols, esters, organic acids, and aldehydes. Among the new technological features, the production of mannoproteins has gained interest. From this perspective, the main aim of this work was to characterize 9 strains of Saccharomyces cerevisiae and 1 of Saccharomyces bayanus for their volatile profiles and the release of mannoproteins. The strains were inoculated in Trebbiano musts and incubated at 15 °C; at the end of fermentation the wines were evaluated by GC/MS/SPME for their volatile profiles and mannoprotein content by enzymatic assay. The strains were inoculated at level ranging between 4.9 and 6.3 log CFU/mL but only the strains L318 and 12233X6167 were able to reach values of 7.5 log CFU/mL. The aromatic profiles resulted in a strain-specific fingerprinting. According to the principal component analysis, the wines produced by the strains L288, L234, and L318 were characterized by the presence of propanoic acid, butanol, octanoic acid, and 3 methyl pentanol while the wine obtained by the strain 12233x35G2 was characterized by the presence of propanoic acid, butanol, octanoic acid and 3 methyl pentanol while the strain 12233x35G2 was characterized by the presence of decanoic acid ethyl ester, heptanoic acid ethyl ester, and acetic acid 2 phenetyl ester. Regarding mannoproteins, the highest concentration was achieved by strain12233x6167 (104 mg/L). The data allowed to select the strains endowed with the best fermentation performances in terms of aroma and mannoproteins release.


1996 ◽  
Vol 7 (6) ◽  
pp. 1571-1572 ◽  
Author(s):  
A. Chadha ◽  
M. Manohar ◽  
T. Soundararajan ◽  
T.S. Lokeswari

2006 ◽  
Vol 281 (7) ◽  
pp. 4446-4456 ◽  
Author(s):  
Sofie M.G. Saerens ◽  
Kevin J. Verstrepen ◽  
Stijn D.M. Van Laere ◽  
Arnout R.D. Voet ◽  
Patrick Van Dijck ◽  
...  

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