Improved furanocoumarin production in Ruta graveolens L. regenerated via in vitro stem internode cultures

2013 ◽  
Vol 7 (3) ◽  
pp. 399-405 ◽  
Author(s):  
Sagarika Bohidar ◽  
Suchismita Pattanaik ◽  
Manikkannan Thiruvanoukkarasu
Nova Scientia ◽  
2017 ◽  
Vol 9 (19) ◽  
pp. 273 ◽  
Author(s):  
Lucía Delgadillo Ruiz ◽  
Rómulo Bañuelos Valenzuela ◽  
Olivia Delgadillo Ruiz ◽  
Mónica Silva Vega ◽  
Perla Gallegos Flores

Introducción: Los extractos de algunas plantas han demostrado tener propiedades antimicrobianas relacionadas a ciertos compuestos químicos como son el timol, carvacrol, limoneno, linalol y terpineno. El objetivo del presente trabajo fue determinar la concentración de estos compuestos en los extractos de Larrea tridentata, Origanum vulgare, Artemisa ludoviciana y Ruta graveolens; así como evaluar su efecto antimicrobiano en Escherichia coli, Acinetobacter baumanii, Pseudomona sp y Staphylococcus aureus.Método: Los extractos se obtuvieron por destilación simple empleando alcohol etílico como solvente, la composición química se evaluó mediante cromatografía de gases. La actividad antimicrobiana de cada uno de los extractos de plantas se realizó por los métodos difusión en pozo y difusión en disco.Resultados: Las bacterias mostraron diferentes grados de sensibilidad a los extractos, presentando inhibición de crecimiento S. aureus con el extracto de O. vulgare y R. graveolens, mientras que la bacteria Pseudomona sp. con los extracto de A. ludoviciana, L. tridentata y O. vulgare.Discusión o Conclusión: La mayor concentración de timol y carvacrol se encontró en los extractos de O. vulgare y L. tridentata. El compuesto linalol se encontró en una proporción mayor en O. vulgare y en menor proporción en A. ludoviciana. Limoneno se encontró en los extractos de O. vulgare y R. graveolens. De las cuatro plantas evaluadas, el extracto de L. tridentata fue mejor debido a que presenta la mayor inhibición en comparación con los otros extractos; y con un efecto similar a los aceites empleados como control. La técnica de difusión en disco, permitió observar mejor los efectos inhibitorios de los extractos y los aceites sobre cada una de las bacterias empleadas en comparación con el método de difusión en pozo.


1999 ◽  
Vol 132 (4) ◽  
pp. 491-498 ◽  
Author(s):  
D. WILMAN ◽  
YILUN JI ◽  
E. J. MTENGETI ◽  
NAZIR AHMAD

In order to learn more about the digestibility, breakdown when eaten and physical structure of stovers and straws, seven diets were compared in one experiment and eight in another. The diets in the first experiment were: the upper and lower parts of lucerne (Medicago sativa) hay, the leaves, upper stem and lower stem of maize (Zea mays) stover and the leaves and stem of sorghum (Sorghum vulgare) stover. The diets in the second experiment were: the upper and lower parts of lucerne hay, the leaves, stems and whole stover of millet (Setaria italica), wheat (Triticum aestivum) straw, rice (Oryza sativa) straw and sweet potato (Ipomoea batatas) haulm. The diets were examined for in vitro digestibility in three forms: milled, chopped (1 cm lengths) and chewed (by cattle). The lengths and widths of the chewed particles were recorded in both experiments. Aspects of the morphology and vascular structure of plant parts of the diets and of chewed particles were recorded in the second experiment.The in vitro digestibility of chopped or chewed plant material was lower (by 11 and 12 percentage units, respectively, on average) than that of milled material. The difference between chewed and milled material in in vitro digestibility was smaller with leaves (5 percentage units, on average) than with stems (18 percentage units, on average) in maize, sorghum and millet. Digestibility of chewed material was similar to or lower than that of chopped material, except with the leaves of maize, sorghum and millet. Millet stover had higher in vitro digestibility than wheat straw or rice straw, whether the diet was milled, chopped or chewed.The thinnest stem internode vascular bundles recorded (0·14 mm) were in wheat. The percentage of stem internode cross-sectional area occupied by vascular bundles was in the range 6–8 for lucerne, millet, wheat and rice. The mean numbers of veins or vascular bundles per chewed particle were in the range 6–23. The mean length of the chewed particles varied from 10 mm with the upper stem of maize to 18 mm with sorghum stem and maize leaves. The mean width of the chewed particles varied from 1·8 mm with rice straw to 5·4 mm with maize leaves.It is suggested that the low digestibility of mature plant tissue such as stovers and straws is not particularly due to thick vascular strands or to a high proportion of vascular tissue, but rather to a high proportion of cell wall and incomplete and delayed access by rumen microorganisms to much of the cell wall.


2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Lara Soares Aleixo de Carvalho ◽  
Lucas Sales Queiroz ◽  
Ismael José Alves Junior ◽  
Ayla das Chagas Almeida ◽  
Elaine Soares Coimbra ◽  
...  

Schistosomiasis is a neglected tropical disease that affects million people worldwide, mostly in developing countries. Ruta graveolens (Rutaceae) is a plant used in folk medicine to treat several diseases, including parasitic infections. In this study, we reported the in vitro schistosomicidal activity of the R. graveolens extract (Rg) and its active fraction (Rg-FAE). Also, the characterization of Rg-FAE by UPLC-ESI-QTOF-MS analysis and its in vitro antileishmanial activity against Leishmania braziliensis were also performed. In vitro schistosomicidal assays were assessed against adult worms of S. mansoni, while cell viability against peritoneal macrophages was measured by MTT assay. Rg (100 μg/mL) exhibited noticeable schistosomicidal activity, causing 100% mortality and decreasing motor activity of all adult male and female schistosomes, but with low activity against L. braziliensis. After chromatographic fractionation of Rg, fraction Rg-FAE was obtained, showing high activity against adult schistosomes. UPLC-ESI-QTOF-MS analysis of Rg-FAE revealed the presence of eleven alkaloids and one furanocoumarin. No significant antileishmanial activity was found for Rg, while Rg-FAE exhibited activity against L. braziliensis promastigotes. We demonstrated, for the first time, that the R. graveolens extract (Rg) and its alkaloid-rich fraction (Rg-FAE) are active against adult worms of S. mansoni, with no significant cytotoxicity on macrophages. Our findings open the route to further antiparasitic studies with the active fraction of R. graveolens and its identified compounds, especially alkaloids.


1986 ◽  
Vol 64 (6) ◽  
pp. 1089-1096 ◽  
Author(s):  
U. Eilert ◽  
B. Wolters ◽  
F. Constabel

Histological analysis of Ruta graveolens L. roots and in vitro grown cell suspensions revealed idioblasts with vacuoles containing clusters of droplets thought to be the storage compartment of acridone alkaloids. These idioblasts contained numerous vacuoles of varying sizes rather than the large, single, central vacuole characteristic of most adjacent parenchyma cells. The structure of idioblasts in roots and suspension cultures was identical. Treatment of suspension cultures with fungal elicitors known to increase alkaloid accumulation greatly did not affect the structure of idioblasts.


2009 ◽  
Vol 35 (3) ◽  
pp. 196-201 ◽  
Author(s):  
Taís Ferreira Almeida ◽  
Margarete Camargo ◽  
Rita de Cássia Panizzi

A flor preta do morangueiro, causada por Colletotrichum acutatum, acarreta sérios problemas à cultura. Com o objetivo de verificar a utilização de extratos vegetais no controle da doença, testes "in vitro" foram realizados com 11 extratos vegetais hidroalcoólicos produzidos de plantas utilizadas na medicina popular. Os extratos foram preparados a partir de diferentes partes da planta, de acordo com a espécie, utilizando água e álcool no processo de extração por maceração. Foi verificada a influência dos extratos no crescimento micelial, esporulação e germinação de esporos de C. acutatum, e também no controle do patógeno em folhas e frutos destacados. De acordo com a metodologia utilizada, os extratos vegetais que apresentaram maior eficiência foram os de folha e ramos de Ruta graveolens, Artemisia absinthium e bulbos de Allium sativum, indicando ter essas plantas potencial fungitóxico para o controle de C. acutatum.


1990 ◽  
Vol 41 (4) ◽  
pp. 719 ◽  
Author(s):  
RA Ballard ◽  
RJ Simpson ◽  
GR Pearce

Changes in the digestibility and chemical composition of a L. rigidum cv. Wimmera sward sown in May, 1985 were measured from 21 d pre-anthesis (9 Oct.) until 69 d after anthesis (7 Jan.) when the plants were dead. Max. yield of 11.7 t DM/ha was reached 8 d pre-anthesis. The in vitro DM digestibility (IVDMD) of whole plants decreased from 58% at anthesis to 36% 69 d after anthesis. This was associated with a decrease in the IVDMD of stem, leaf blades and sheaths. In the 3rd stem internode, which was considered representative of the stem, the loss of digestible yield was due to loss of DM soluble in neutral detergent (NDS). The NDS consisted mainly of non-structural carbohydrates. Similar losses of NDS contributed to loss of digestibility in the uppermost leaf blade and leaf sheath. The digestibility of NDS was generally 80-95% but in the leaf blade this declined to 45% as NDS was mobilized during leaf senescence. NDF digestibility of the stem declined from 35% at anthesis to 19% when dead; corresponding values for the uppermost leaf blade were 83 and 54%, resp., and for the leaf sheath 46 and 37%, resp. These characteristics of a senescing grass sward are discussed in relation to options for improving digestibility of dead grass pastures.


2000 ◽  
Vol 69 (4) ◽  
pp. 466-471
Author(s):  
Tadashi Fujioka ◽  
Masayoshi Fujita ◽  
Yoshiki Miyamoto

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