In situ Demonstration and Characteristic Analysis of the Protease Components from Marine Bacteria Using Substrate Immersing Zymography

2014 ◽  
Vol 175 (1) ◽  
pp. 489-501 ◽  
Author(s):  
Dan Liu ◽  
XingHao Yang ◽  
JiaFeng Huang ◽  
RiBang Wu ◽  
CuiLing Wu ◽  
...  
Keyword(s):  
Marine Bacteria ◽  
Characteristic Analysis

Development of a microbe domestication pod (MD Pod) for in situ cultivation of micro‐encapsulated marine bacteria

2020 ◽  
Author(s):  
Tartela Alkayyali ◽  
Emily Pope ◽  
Sydney K. Wheatley ◽  
Christopher Cartmell ◽  
Bradley Haltli ◽  
...  
Keyword(s):  
Marine Bacteria

scholarly journals Use of Microautoradiography Combined with Fluorescence In Situ Hybridization To Determine Dimethylsulfoniopropionate Incorporation by Marine Bacterioplankton Taxa

2004 ◽  
Vol 70 (8) ◽  
pp. 4648-4657 ◽  
Author(s):  
Maria Vila ◽  
Rafel Simó ◽  
Ronald P. Kiene ◽  
Jarone Pinhassi ◽  
José M. González ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gulf Of Mexico ◽  
Fluorescence In Situ Hybridization ◽  
Marine Bacteria ◽  
Heterotrophic Bacteria ◽  
Phytoplankton Bloom ◽  
Mediterranean Coast ◽  
Marine Bacterioplankton ◽  
Significant Uptake

ABSTRACT The fraction of planktonic heterotrophic bacteria capable of incorporating dissolved dimethylsulfoniopropionate (DMSP) and leucine was determined at two coastal sites by microautoradioagraphy (AU). In Gulf of Mexico seawater microcosm experiments, the proportion of prokaryotes that incorporated sulfur from [35S]DMSP ranged between 27 and 51% of 4′,6-diamidino-2-phenylindole (DAPI)-positive cells, similar to or slightly lower than the proportion incorporating [3H]leucine. In the northwest Mediterranean coast, the proportion of cells incorporating sulfur from [35S]DMSP increased from 5 to 42% from January to March, coinciding with the development of a phytoplankton bloom. At the same time, the proportion of cells incorporating [3H]leucine increased from 21 to 40%. The combination of AU and fluorescence in situ hybridization (FISH) revealed that the Roseobacter clade (α-proteobacteria) accounted for 13 to 43% of the microorganisms incorporating [35S]DMSP at both sampling sites. Significant uptake of sulfur from DMSP was also found among members of the γ-proteobacteria and Cytophaga-Flavobacterium groups. Roseobacter and γ-proteobacteria exhibited the highest percentage of DAPI-positive cells incorporating 35S from DMSP (around 50%). Altogether, the application of AU with [35S]DMSP combined with FISH indicated that utilization of S from DMSP is a widespread feature among active marine bacteria, comparable to leucine utilization. These results point toward DMSP as an important substrate for a broad and diverse fraction of marine bacterioplankton.

Fluorescence in situ hybridization biomarkers of gastric cancer risk in intestinalized Helicobacter pylori pangastritis.

2011 ◽  
Vol 29 (4_suppl) ◽  
pp. 17-17
Author(s):  
B. Kim ◽  
M. P. Bronner
Keyword(s):  
Gastric Cancer ◽  
Optimal Choice ◽  
Antral Mucosa ◽  
Characteristic Analysis ◽  
Chromosomal Alterations ◽  
Interphase Fish ◽  
Gastric Neoplasia ◽  
H Pylori ◽  
Antral Biopsy

17 Background: H. pylori (HP) intestinalized pangastritis predisposes to gastric adenocarcinoma. Biomarkers to identify the less than 10% subset of chronic gastritis patients who will develop gastric cancer are greatly needed to focus surveillance efforts onto those patients most likely to benefit. Investigations in ulcerative colitis and Barrett's esophagus reveal that chromosomal alterations in far distant and nondysplastic mucosa are powerful cancer biomarkers. We hypothesized a similar situation in gastric tumorigenesis. Methods: The full morphologic spectrum of gastric neoplasia was examined using flow-sorted gastric epithelial cells from 10 gastric cancer resections versus 20 normal gastric control patients. Then we addressed whether we could distinguish the subset of HP intestinalized pangastritis patients who develop cancer (progressors), from those who remain neoplasia-free (nonprogressors). A single nondysplastic intestinalized gastric antral biopsy collected using chromoendoscopy on each of 41 Korean progressors with gastric cancer and 30 nonprogressors without dysplasia. Interphase FISH on whole epithelial cell preparations using 4q32-q33, 10q22.1, 18q21.2 and 19p11-q11 chromosomal arm probes was performed. Results: Increasing chromosomal alterations occurred throughout the morphologic spectrum of gastric neoplasia. Significantly increased FISH alterations for chromosomes 4q32-q33 (p = 0.016) and 10q22.1 (p = 0.028) occurred as early in the neoplastic spectrum as nondysplastic distant intestinal metaplasia. Subsequent nondysplastic antral biopsy testing of progressors versus nonprogressors revealed parallel FISH results for 4q32-q33 (p = 0.039) and 10q22.1 (p = 0.023), and 19p11-q11 (p = 0.019). Receiver operator characteristic analysis for these 3 combined probes demonstrated a sensitivity of 0.53 and specificity of 0.92 with optimal choice of thresholds. Conclusions: Interphase FISH for 4q32-q33, 10q22.1, and 19p11-q11 chromosomal probes on nondysplastic intestinalized antral mucosa shows promise as a biomarker of gastric cancer in multifocal HP intestinalized pangastritis. No significant financial relationships to disclose.

Simulated in situ growth rates of pelagic marine bacteria

1978 ◽  
Vol 65 (10) ◽  
pp. 541-542 ◽  
Author(s):  
A. F. Carlucci ◽  
P. M. Williams
Keyword(s):  
Marine Bacteria ◽  
Growth Rates ◽  
In Situ Growth

Activity of marine bacteria under incubated and in situ conditions

1999 ◽  
Vol 20 ◽  
pp. 213-223 ◽  
Author(s):  
EB Sherr ◽  
BF Sherr ◽  
CT Sigmon
Keyword(s):  
Marine Bacteria ◽  
In Situ Conditions

Simulated or measured soil moisture: which one is adding more value to regional landslide early warning?

2021 ◽  
Vol 25 (8) ◽  
pp. 4585-4610
Author(s):  
Adrian Wicki ◽  
Per-Erik Jansson ◽  
Peter Lehmann ◽  
Christian Hauck ◽  
Manfred Stähli
Keyword(s):  
Soil Moisture ◽  
Early Warning ◽  
Prediction Models ◽  
Forecast Model ◽  
Early Warning Systems ◽  
Transfer Model ◽  
Characteristic Analysis ◽  
Statistical Framework ◽  
Landslide Early Warning

Abstract. The inclusion of soil wetness information in empirical landslide prediction models was shown to improve the forecast goodness of regional landslide early warning systems (LEWSs). However, it is still unclear which source of information – numerical models or in situ measurements – is of higher value for this purpose. In this study, soil moisture dynamics at 133 grassland sites in Switzerland were simulated for the period of 1981 to 2019, using a physically based 1D soil moisture transfer model. A common parameterization set was defined for all sites, except for site-specific soil hydrological properties, and the model performance was assessed at a subset of 14 sites where in situ soil moisture measurements were available on the same plot. A previously developed statistical framework was applied to fit an empirical landslide forecast model, and receiver operating characteristic analysis (ROC) was used to assess the forecast goodness. To assess the sensitivity of the landslide forecasts, the statistical framework was applied to different model parameterizations, to various distances between simulation sites and landslides and to measured soil moisture from a subset of 35 sites for comparison with a measurement-based forecast model. We found that (i) simulated soil moisture is a skilful predictor for regional landslide activity, (ii) that it is sensitive to the formulation of the upper and lower boundary conditions, and (iii) that the information content is strongly distance dependent. Compared to a measurement-based landslide forecast model, the model-based forecast performs better as the homogenization of hydrological processes, and the site representation can lead to a better representation of triggering event conditions. However, it is limited in reproducing critical antecedent saturation conditions due to an inadequate representation of the long-term water storage.

scholarly journals In-Situ Monitoring and Characteristic Analysis of Freezing-Thawing Cycles in a Deep Vadose Zone

Water ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1261
Author(s):  
Ce Zheng ◽  
Yudong Lu ◽  
Xiuhua Liu ◽  
Jiří Šimůnek ◽  
Yijian Zeng ◽  
...  
Keyword(s):  
Soil Temperature ◽  
Water Content ◽  
Vadose Zone ◽  
In Situ Monitoring ◽  
Characteristic Analysis ◽  
Soil Layers ◽  
Vapor Flux ◽  
Freeze Thaw ◽  
Soil Temperature And Moisture

Freeze-thaw cycles play a critical role in affecting ecosystem services in arid regions. Monitoring studies of soil temperature and moisture during a freeze-thaw process can generate data for research on the coupled movement of water, vapor, and heat during the freezing-thawing period which can, in turn, provide theoretical guidance for rational irrigation practices and ecological protection. In this study, the soil temperature and moisture changes in the deep vadose zone were observed by in-situ monitoring from November 2017 to March 2018 in the Mu Us Desert. The results showed that changes in soil temperatures and temperature gradients were largest in soil layers above the 100-cm depth, and variations decreased with soil depth. The relationship between soil temperature and unfrozen water content can be depicted well by both theoretical and empirical models. Due to gradients of the matric potential and temperature, soil water flowed from deeper soil layers towards the frozen soil, increasing the total water content at the freezing front. The vapor flux, which was affected mainly by temperature, showed diurnal variations in the shallow 20-cm soil layer, and its rate and variations decreased gradually with increasing soil depths. The freeze-thaw process can be divided into three stages: the initial freezing stage, the downward freezing stage, and the thawing stage. The upward vapor flux contributed to the formation of the frozen layer during the freezing process.

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