Study on the Characteristics of Intestinal Flora Composition in Gastric Cancer Patients and Healthy People in the Qinghai-Tibet Plateau

AbstractThe aim of this study is to compare and analyze the structure and diversity of intestinal flora between gastric cancer patients and healthy people in the Qinghai-Tibet Plateau and to explore the characteristics of the intestinal flora composition in gastric cancer patients in the plateau area, and to determine the possible correlation between the intestinal flora and gastric cancer. Fresh feces from 22 cases of gastric cancer patients diagnosed in a tertiary hospital in Qinghai Province and 30 cases of healthy people during the same period were collected. The 52 subjects were undergone for 16S rDNA gene sequencing of intestinal bacteria to analyze and compare the diversity and compositional characteristics of intestinal flora. Analysis of the diversity of intestinal flora between the gastric cancer group and the healthy group was based on the Chao1 index of species richness, Shannon diversity index, and Simpson index. It showed that the gastric cancer group had no statistically difference from the healthy group (P > 0.05). In the Venn diagram, the number of OTU units shared by the gastric cancer group and the healthy group is 6997, and the number of unique OTU units in the healthy group is 2282, while the number of OTU units in the gastric cancer group is 896 and the difference is statistically significant (χ2 = 495.829), P < 0.000). Analysis of the composition and abundance distribution of intestinal flora showed that at the phylum level, there is no significant deference in abundance between the healthy group of Bacteroides and Firmicutes compared with the gastric cancer group (P > 0.05). However, there is a statistically significant difference in abundance between the healthy groups of Proteobacteria compared with the gastric cancer group (P < 0.05). At the genus level, the gastric cancer group of Prevotella_9 is significantly different from the healthy group (P < 0.05). Meanwhile, the gastric cancer group of Streptococcus and Lactobacillus are significantly different from the healthy group (P < 0.001). There are differences in the composition and abundance of intestinal flora between patients with gastric cancer and healthy people in plateau areas, suggesting that Proteobacteria, Prevotella_9, Streptococcus, and Lactobacillus have increased in the Qinghai-Tibet Plateau and becoming one of the factors related to the incidence of gastric cancer in the region.

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Expression and Clinical Significance of Serum Exosomal miR-375 in Patients with Gastric Cancer

Tobacco Regulatory Science ◽

10.18001/trs.7.5.1.162 ◽

2021 ◽

Vol 7 (5) ◽

pp. 3896-3904

Author(s):

Daoting Deng ◽

Hong Zhang ◽

Junxi Liu ◽

Lina Ma ◽

Xinrui Lei ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Patients ◽

Cox Regression ◽

Prognostic Significance ◽

Cox Regression Analysis ◽

Healthy Group ◽

Cancer Group ◽

Kaplan Meier ◽

Gastric Cancer Patients ◽

Gastric Cancer Group

To explore exosomal miR-375 expression in gastric cancer patients and its relationship with patient prognosis. A total of 53 patients diagnosed with gastric cancer in our hospital from May 2014 to May 2016 were included as the gastric cancer group, and 46 healthy women who came to our hospital for physical examination during the same period were enrolled as the healthy group. Exosomal miR-375 expression level was detected using qRT-PCR, and the diagnostic performance and prognostic significance of exosomal miR-375 in gastric cancer were explored. The gastric cancer group showed increased exosomal miR-375 expression than the healthy group (P< 0.05); Kaplan-Meier survival analysis exhibited that serum exosomal miR-375 has an AUC of 0.778, sensitivity of 69.57%, and specificity of 75.47%, whereas Cox regression analysis showed that the miR-375 expression in exosomes was an independent risk factor affecting the prognosis of gastric cancer patients (P< 0.05). Patient with gastric cancer showed upregulated miR-375 expression in serum exosomes. Serum exosomal miR-375 was found to has positive sensitivity and specificity in the diagnosis of gastric cancer, which may be associated with poor prognosis of gastric cancer patients.

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The effect of CTLA-4 and CD28 gene variants and circulating protein levels in patients with gastric cancer

Turkish Journal of Biochemistry ◽

10.1515/tjb-2017-0024 ◽

2017 ◽

Vol 42 (5) ◽

Author(s):

Soykan Arikan ◽

Alper Gümüş ◽

Özlem Küçükhüseyin ◽

Cihan Coşkun ◽

Saime Turan ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Patients ◽

Control Group ◽

Protein Levels ◽

Cancer Group ◽

Pcr Rflp ◽

Gastric Cancer Patients ◽

Turkish Patients ◽

Circulating Levels ◽

Gastric Cancer Group

AbstractObjective:Gastric cancer is one of the most common malignancies worldwide. The risk factors for gastric cancer include environmental and genetic factors. Inflammation and the immune system are known to contribute to the development of the gastric cancer. We examined the influence of critical polymorphisms of CTLA-4 and CD28 genes and circulating protein levels on the etiology of gastric cancer.Methods:Genotyping of SNPs was performed in 55 gastric cancer patients and 105 healthy individuals using the PCR-RFLP method, and circulating levels of sCTLA-4 and sCD28 were measured.Results:There were no significant differences in the genotype and allele distributions of the evaluated SNPs [CTLA-4-318 C>T (rs5742909), CTLA-4+49 A>G (rs231775), CD28 C>T (rs3116496)] between gastric cancer patients and controls (p=0.36, p=0.78, and p=0.80, respectively). The circulating levels of sCTLA-4 and sCD28 were significantly different between the gastric cancer group and the control group (p<0.001 and p<0.001, respectively).Conclusion:The present results suggest that the CTLA-4 and CD28 gene polymorphisms that were evaluated do not play an important role in Turkish patients with gastric cancer. However, sCTLA4 and sCD28 levels were higher in cancer patients and may be useful as an auxiliary parameter in the diagnosis and monitoring of gastric cancer.

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Increased Oxidative Stress in Gastric Cancer Patients and Their First-Degree Relatives: A Prospective Study from North-Eastern Brazil

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/6657434 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Manuel B. Braga-Neto ◽

Deiziane V. S. Costa ◽

Dulciene M. M. Queiroz ◽

Felipe S. Maciel ◽

Michelle S. de Oliveira ◽

...

Keyword(s):

Oxidative Stress ◽

Gastric Cancer ◽

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Cancer Patients ◽

Catalase Activity ◽

First Degree Relatives ◽

Cancer Group ◽

Gastric Cancer Patients ◽

Gastric Cancer Group

Background and Aims. First-degree relatives of gastric cancer patients are at increased risk of developing gastric cancer. Increased oxidative stress, including lipid peroxidation, has been associated with gastric carcinogenesis. Whether first-degree relatives of gastric cancer patients have increased oxidative stress remains unknown. We aimed to compare oxidative stress in patients with gastric cancer, their first-degree relatives, and dyspeptic controls. Methods. A total of 155 patients undergoing upper endoscopy were prospectively enrolled, including 50 with gastric cancer, 49 first-degree relatives of gastric cancer patients, and 56 controls. Serum concentrations of malondialdehyde (MDA) and glutathione) and activities of superoxide dismutase (SOD) and catalase were measured. Multivariate analysis adjusting for sex, age, smoking status, and alcohol consumption was performed. Results. Lipid peroxidation, as measured by concentration of MDA (nmol/mL), was higher ( p = 0.04 ), and glutathione levels were lower ( p < 0.001 ) in the gastric cancer group compared to controls. There was no difference in the catalase activity among the groups. There was no difference in glutathione and MDA concentration or catalase activity between the different stages of gastric cancer based on the TNM classification. Relatives of gastric cancer patients had higher glutathione concentration (μmol/mL) compared to gastric cancer patients (262.5 vs. 144.6; p = 0.018 ), while there was no difference in MDA concentration. Catalase and superoxide dismutase activity were lower in the gastric cancer group (3.82 vs. 0.91; p < 0.001 and 1.04 vs. 0.6; p < 0.001 ) compared to their first-degree relatives. Interestingly, MDA concentration in the first-degree relative group was higher than in the control group (7.9 vs. 5.1; p = 0.03 ). Conclusions. In this study, similarly to gastric cancer patients, their first-degree relatives were found to have increased oxidative stress compared to controls. Further studies are warranted to validate this observation and to better understand the role of oxidative stress as a possible biomarker in this population.

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DETERMINATION OF P53 GENE CODON 72 POLYMORPHISMS IN PATIENTS WITH GASTRIC CANCER

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2013.2.2 ◽

2013 ◽

pp. 11-17

Author(s):

Thi Tuy Ha Nguyen ◽

Thi Minh Thi Ha

Keyword(s):

Gastric Cancer ◽

Cancer Patients ◽

P53 Gene ◽

Diffuse Gastric Cancer ◽

Codon 72 ◽

Cancer Group ◽

Pcr Rflp ◽

Gastric Cancer Patients ◽

The Relationship

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.

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Discovering Biomarkers in Gastric Cancer by Urine Metabolomics

10.21203/rs.3.rs-820790/v1 ◽

2021 ◽

Author(s):

Yuan Kong ◽

Hongya Zhang ◽

Shuang Li ◽

Jian Suo ◽

Shaopeng Zhang ◽

...

Keyword(s):

Gastric Cancer ◽

Cluster Analysis ◽

Early Gastric Cancer ◽

Partial Least Square ◽

Least Square ◽

Urine Samples ◽

Diagnostic Ability ◽

Healthy Group ◽

Cancer Group ◽

Gastric Cancer Group

Abstract IntroductionGastric cancer is one of the most common gastrointestinal tumors, ranking forth in incidence and second in mortality worldwide. Discovering molecular biomarkers for early gastric cancer diagnosis is of great importance. MethodsUrine and related clinical data of 40 patients with gastric cancer (20 in advanced stage and 20 in early stage) and 20 healthy volunteers from Jilin University First Hospital were collected. Liquid chromatography-mass spectrometry (LC-MS) was used to detect urine samples and the metabolic differences between the three groups of urine samples were analyzed. The principal component analysis was performed after data processing, and different metabolites were found using analysis of variance. Partial least square discriminant analysis was performed to further narrow the range of different metabolites. The precise mass to charge ratios of different metabolites were imported into the Human Metabolomics Database (HMDB). Finally, the identified different metabolites were further screened by cluster analysis and ROC curve. ResultsUrine samples of the healthy group (NOR), the early gastric cancer group (EGC), and the advanced gastric cancer group (AGC) were different metabolites. 324 statistically significant metabolites are screened out. The cluster analysis showed 7-Methylguanine, vinylacetylglycine, butyric acid, 4-Vinylphenol sulf, 5`-biotinyl-AMP, and 3-Amino-2-piperido in EGC, AGC and NGO were similar. 7-Methylguanine, vinylacetylglycine and 4-Vinylphenolsulfate had good diagnostic ability in EGC and NOR (p<0.05), and gastric cancer and NOR (p<0.05). ConclusionDifferences in the metabolites in urine between the early gastric cancer group and the healthy group were found. 7-Methylguanine, Vinylacetylglycine, and 4-Vinylphenolsulfate have good diagnostic ability and may be potential biomarkers of early gastric cancer.

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Platelets and Inflammatory Markers in Patients with Gastric Cancer

Clinical and Developmental Immunology ◽

10.1155/2013/401623 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 24

Author(s):

Joanna Matowicka-Karna ◽

Zbigniew Kamocki ◽

Beata Polińska ◽

Joanna Osada ◽

Halina Kemona

Keyword(s):

Gastric Cancer ◽

Inflammatory Markers ◽

Metastatic Cancer ◽

Fold Increase ◽

Tumor Stage ◽

Cancer Group ◽

Group A ◽

Gastric Cancer Patients ◽

Gastric Cancer Group ◽

Development Of Gastric Cancer

The aim of the study was to assess the contribution of platelets and inflammatory markers in gastric cancer. We studied 50 patients. Taking into consideration the advancement of gastric cancer, patients were divided into 3 groups. Group (E)—13 patients with early gastric cancer, group (A)—18 patients with regionally advanced cancer, and group (M)—19 patients with metastatic cancer. The determinations were performed twice prior to surgery and after surgery. In patients with gastric cancer, there is an increase in IL-6 and IL-23 compared with the healthy group. The highest values of IL-6 were obtained in early cancer (more than 8-fold increase), which seems to confirm the presence of acute inflammation. The lowest value of both of these cytokines was obtained in patients with metastatic cancer. In all patients, regardless of tumor stage, there was an increase in the concentration of CRP. An increase of PLT, higher proportion of the percentage of large platelets (LPLT), and increased mean platelet volume (MPV) were observed in the process of disease development. A positive correlation between MPV and LPLT and the accompanying decrease in the concentration of proinflammatory cytokines indicates the presence of an existing relationship between the platelet morphological parameters and the inflammation process in the development of gastric cancer.

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Diagnostic value of MRI-DWI signal intensity value combined with serum PGI, PGII and CA199 in early gastric cancer

Cellular and Molecular Biology ◽

10.14715/cmb/2021.67.2.14 ◽

2021 ◽

Vol 67 (2) ◽

pp. 95-100

Author(s):

Minhong Li ◽

GuangHui Zheng ◽

Lin Yu ◽

Li-lian Tan ◽

Xi Li ◽

...

Keyword(s):

Gastric Cancer ◽

Early Gastric Cancer ◽

Signal Intensity ◽

Statistical Significance ◽

Gastric Wall ◽

Diagnostic Value ◽

Wall Thickening ◽

Healthy Group ◽

Cancer Group ◽

Gastric Cancer Group

To explore the diagnostic value of MRI-DWI signal intensity value combined with serum PGI. PGII and CA199 in early gastric cancer. Sixty cases of gastric cancer patients admitted to our hospital from December 2019 to December 2020 were selected as the gastric cancer group and 80 cases of healthy volunteers who underwent physical examination in our hospital during the same period were selected as the healthy group. All the 60 patients underwent MRI-DWI examination, and the pathological diagnosis results were regarded as the gold standard. MRI-DWI images, MRI-DWI signal intensity values of patients with different degrees of gastric cancer differentiation. Serum PGI, PGII and CA199 levels of subjects in the two groups were compared. AUC was used to evaluate the diagnostic value of MRI-DWI signal intensity value combined with serum PGI, PG II and CA199 for early gastric cancer. In the healthy group, T1W1 showed relatively uniform low signal intensity. While T2WI showed no significant increase in signal intensity. In the gastric cancer group. There was diffuse gastric wall thickening, local thickening or mass formation; T1WI and WATS showed slightly lower signal intensity in the lesion area. T2WI, FLAIR and B-TFE showed slightly uneven or moderately increased signal intensity. DWI showed limited diffusion, and the signal intensity increased uniformly or more uniformly, and the range of increase was clear. The signal intensity of MRI-DWI was 89.12 ± 8.14 in patients with low differentiation, 82.17 ± 6.35 in patients with moderate differentiation, and 74.52 ± 4.53 in patients with high differentiation. There were significant differences in the signal intensity of MRI-DWI among the three groups, and the difference was statistically significant (F=12.214, P <0.05). Serum PGI levels of subjects in the gastric cancer group were significantly lower than those in the healthy group, and the levels of PGII and CA199 were significantly higher than that in the healthy group, with statistical significance (P <0.05). The AUC, sensitivity and specificity of MRI-DWI signal intensity value and serum PGI, PGII and CA199 combined indexes in the diagnosis of gastric cancer were significantly higher than those of the independent indexes, with statistical significance (P <0.05). Conclusion: MRI-DWI signal strength value, serum PGI, PGII and CA199 levels are closely related to the occurrence and development of early gastric cancer. The combined detection and diagnosis efficiency is higher, which is helpful to improve the detection rate of early gastric cancer and is worthy of extensive clinical application.

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