Boron Attenuates Heat Stress–Induced Apoptosis by Inhibiting Endoplasmic Reticulum Stress in Mouse Granulosa Cells

2020 ◽  
Vol 199 (2) ◽  
pp. 611-621 ◽  
Author(s):  
Yongjie Xiong ◽  
Erhui Jin ◽  
Qirun Yin ◽  
Chuanyan Che ◽  
Shaojun He
Keyword(s):  
Endoplasmic Reticulum ◽  
Heat Stress ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Induced Apoptosis

Oxidative and endoplasmic reticulum stress defense mechanisms of bovine granulosa cells exposed to heat stress

2018 ◽  
Vol 110 ◽  
pp. 130-141 ◽  
Author(s):  
Teshome Wondie Alemu ◽  
Hari Om Pandey ◽  
Dessie Salilew Wondim ◽  
Samuel Gebremedhn ◽  
Christiane Neuhof ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Heat Stress ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Defense Mechanisms ◽  
Stress Defense

scholarly journals NRF2-mediated signaling is a master regulator of transcription factors in bovine granulosa cells under oxidative stress condition

2021 ◽  
Author(s):  
Mohamed Omar Taqi ◽  
Mohammed Saeed-Zidane ◽  
Samuel Gebremedhn ◽  
Dessie Salilew-Wondim ◽  
Ernst Tholen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Endoplasmic Reticulum ◽  
Transcription Factors ◽  
Endoplasmic Reticulum Stress ◽  
Granulosa Cells ◽  
Mitochondrial Activity ◽  
Small Interference Rna ◽  
Nrf2 Signaling Pathway

AbstractTranscription factors (TFs) are known to be involved in regulating the expression of several classes of genes during folliculogenesis. However, the regulatory role of TFs during oxidative stress (OS) is not fully understood. The current study was aimed to investigate the regulation of the TFs in bovine granulosa cells (bGCs) during exposure to OS induced by H2O2 in vitro. For this, bGCs derived from ovarian follicles were cultured in vitro till their confluency and then treated with H2O2 for 40 min. Twenty-four hours later, cells were subjected to various phenotypic and gene expression analyses for genes related to TFs, endoplasmic reticulum stress, apoptosis, cell proliferation, and differentiation markers. The bGCs exhibited higher reactive oxygen species accumulation, DNA fragmentation, and endoplasmic reticulum stress accompanied by reduction of mitochondrial activity after exposure to OS. In addition, higher lipid accumulation and lower cell proliferation were noticed in H2O2-challenged cells. The mRNA level of TFs including NRF2, E2F1, KLF6, KLF9, FOS, SREBF1, SREBF2, and NOTCH1 was increased in H2O2-treated cells compared with non-treated controls. However, the expression level of KLF4 and its downstream gene, CCNB1, were downregulated in the H2O2-challenged group. Moreover, targeted inhibition of NRF2 using small interference RNA resulted in reduced expression of KLF9, FOS, SREBF2, and NOTCH1 genes, while the expression of KLF4 was upregulated. Taken together, bovine granulosa cells exposed to OS exhibited differential expression of various transcription factors, which are mediated by the NRF2 signaling pathway.

Heat stress aggravates oxidative stress, apoptosis, and endoplasmic reticulum stress in the cerebellum of male C57 mice

2021 ◽  
Author(s):  
Hajar Oghbaei ◽  
Leila Hosseini ◽  
Fereshteh Farajdokht ◽  
Sepideh Rahigh Aghsan ◽  
Alireza Majdi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endoplasmic Reticulum ◽  
Heat Stress ◽  
Endoplasmic Reticulum Stress
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