Deciphering the Epitranscriptomic Signatures in Cell Fate Determination and Development

2019 ◽  
Vol 15 (4) ◽  
pp. 474-496
Author(s):  
Varun Haran ◽  
Nibedita Lenka
Keyword(s):  
Cell Fate ◽  
Cell Fate Determination ◽  
Fate Determination

scholarly journals DISTAG/TBCCd1 Is Required for Basal Cell Fate Determination in Ectocarpus

2017 ◽  
Vol 29 (12) ◽  
pp. 3102-3122 ◽  
Author(s):  
Olivier Godfroy ◽  
Toshiki Uji ◽  
Chikako Nagasato ◽  
Agnieszka P. Lipinska ◽  
Delphine Scornet ◽  
...  
Keyword(s):  
Cell Fate ◽  
Basal Cell ◽  
Cell Fate Determination ◽  
Fate Determination

scholarly journals The Natural and Engineered 3D Microenvironment as a Regulatory Cue During Stem Cell Fate Determination

2009 ◽  
Vol 15 (3) ◽  
pp. 371-380 ◽  
Author(s):  
Amanda W. Lund ◽  
Bülent Yener ◽  
Jan P. Stegemann ◽  
George E. Plopper
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Cell Fate Determination ◽  
Stem Cell Fate ◽  
Fate Determination ◽  
3D Microenvironment

scholarly journals Cooperation of BMP and IHH signaling in interdigital cell fate determination

PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0197535 ◽  
Author(s):  
Arunima Murgai ◽  
Sara Altmeyer ◽  
Stephanie Wiegand ◽  
Przemko Tylzanowski ◽  
Sigmar Stricker
Keyword(s):  
Cell Fate ◽  
Cell Fate Determination ◽  
Fate Determination

IMMU-50. GLIOBLASTOMA MANIPULATES HEMATOPOIETIC STEM CELL DIFFERENTIATION OUTCOMES AND DRIVES ALTERED IMMUNE RECONSTITUTION

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi104-vi104
Author(s):  
Bayli DiVita Dean ◽  
Tyler Wildes ◽  
Joseph Dean ◽  
David Shin ◽  
Connor Francis ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Progenitor Cells ◽  
Rna Sequencing ◽  
Cell Fate ◽  
Immune Reconstitution ◽  
Stem Cell Differentiation ◽  
Cell Fate Determination ◽  
Hematopoietic Stem ◽  
Fate Determination

Abstract INTRODUCTION Bone marrow-derived hematopoietic stem and progenitor cells (HSPCs) give rise to the cellular components of the immune system. Unfortunately, immune reconstitution from HSPCs are negatively impacted by solid cancers, including high-grade gliomas. For example, an expansion of myeloid progenitor cells has been previously described across several cancers that originate outside the CNS. A similar expansion of MDSCs coupled with diminished T cell function has also been described in the peripheral blood of patients with newly-diagnosed GBM. Alterations in both lymphoid and myeloid compartments due to CNS malignancy led us to determine how intracranial gliomas impact HSPCs in both their capacity to reconstitute the immune compartment and in their cell fate determination. This is important to better understand the impact of gliomas on immunity and how we can leverage these findings to better develop cellular immunotherapeutics. METHODS HSPCs were isolated from bone marrow of C57BL/6 mice with orthotopic KR158B glioma, or age-matched naïve mice. Experiments were conducted to compare relative changes in: gene expression (RNA-sequencing), precursor frequencies, cell fate determination, and cellular function of cells derived from HSPCs of glioma-bearing mice. RESULTS RNA-sequencing revealed 700+ genes whose expression was significantly up- or downregulated in HSPCs from glioma-bearing mice, particularly those involved with stemness and metabolic activity. Importantly, HSPCs from glioma-bearing mice expressed upregulation of genes involved in myelopoiesis relative to naïve mice. This was coupled with an expansion of granulocyte macrophage precursors (GMPs), the progenitors to gMDSCs. Next, differentiation assays revealed that HSPCs from glioma-bearing mice had higher propensity of differentiating into MDSC under homeostatic conditions relative to controls both in vitro and in vivo. Furthermore, mice bearing intracranial gliomas possess an expansion of MDSCs which are more suppressive on T cell proliferation and hinders T cell-mediated tumor cell killing relative to MDSCs derived from naïve control mice.

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