The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion

2014 ◽  
Vol 31 (3) ◽  
Author(s):  
Nina Petrović ◽  
Vesna Mandušić ◽  
Boban Stanojević ◽  
Silvana Lukić ◽  
Lidija Todorović ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Invasion ◽  
Breast Cancers ◽  
Expression Levels ◽  
Non Invasive ◽  
Breast Cancer Invasion ◽  
The Difference

Suppressions of metastatic breast cancer invasion and metastasis to brain/cross talk HER2/ERK1/2/MMP-9 signaling pathway

2018 ◽  
Vol 6 (4) ◽  
pp. 349-361
Author(s):  
Yu-Chun Lin ◽  
Dong-Qing Chin
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Signaling Pathway ◽  
Metastatic Breast ◽  
Cancer Invasion ◽  
Breast Cancer Cell Lines ◽  
Breast Cancers ◽  
Invasion And Metastasis ◽  
Matrigel Invasion ◽  
Breast Cancer Invasion

Understanding the molecular pathways that contribute to the development of metastatic breast cancer invasion and metastasis to brain is needed to improve the clinical utility of novel agents, and to predict the success of targeted personalized therapy based on tumor-specific mutations. Little is known about the clinical significance of HER2/ERK1/2/MMP-9 signaling pathway in breast cancer. We performed Global exon array to study the expression of ERK1/2/MMP-9 signaling pathway in metastatic breast cancer to brain, compared its expression in primary breast cancer and breast cancers metastatic to other organs, and validated the findings by RT-PCR. Immunohistochemistry was performed to study the expression and localization of ERK1/2/MMP-9 proteins in primary and metastatic breast cancer tissues and breast cancer cell lines. We performed matrigel invasion, transendothelial migration and membrane potential assays in established lines of normal breast cells.

Suppressions of metastatic breast cancer invasion and metastasis to brain/cross talk HER2/ERK1/2/MMP-9 signaling pathway

2016 ◽  
Vol 4 (2) ◽  
pp. 227-239
Author(s):  
Yu-Chun Lin ◽  
Dong-Qing Chin
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Signaling Pathway ◽  
Metastatic Breast ◽  
Cancer Invasion ◽  
Breast Cancer Cell Lines ◽  
Breast Cancers ◽  
Invasion And Metastasis ◽  
Matrigel Invasion ◽  
Breast Cancer Invasion

Understanding the molecular pathways that contribute to the development of metastatic breast cancer invasion and metastasis to brain is needed to improve the clinical utility of novel agents, and to predict the success of targeted personalized therapy based on tumor-specific mutations. Little is known about the clinical significance of HER2/ERK1/2/MMP-9 signaling pathway in breast cancer. We performed Global exon array to study the expression of ERK1/2/MMP-9 signaling pathway in metastatic breast cancer to brain, compared its expression in primary breast cancer and breast cancers metastatic to other organs, and validated the findings by RT-PCR. Immunohistochemistry was performed to study the expression and localization of ERK1/2/MMP-9 proteins in primary and metastatic breast cancer tissues and breast cancer cell lines. We performed matrigel invasion, transendothelial migration and membrane potential assays in established lines of normal breast cells.

scholarly journals A Non-Invasive Metabolic Investigation of Breast Cancer Invasion

2019 ◽  
Vol 116 (3) ◽  
pp. 550a
Author(s):  
Austin E.Y.T. Lefebvre ◽  
Freddie A. Adame ◽  
Michelle A. Digman
Keyword(s):  
Breast Cancer ◽  
Cancer Invasion ◽  
Non Invasive ◽  
Breast Cancer Invasion ◽  
Metabolic Investigation

scholarly journals Pre-metastatic niche drives breast cancer invasion by modulating MSC homing and CAF differentiation

2021 ◽  
Author(s):  
Neha Saxena ◽  
Garvit Bhardwaj ◽  
Sameer Jadhav ◽  
Hamim Zafar ◽  
Shamik Sen
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Cancer Progression ◽  
Cancer Invasion ◽  
Shear Stresses ◽  
Dependent Manner ◽  
Breast Cancers ◽  
Cancer Associated Fibroblast ◽  
Breast Cancer Invasion ◽  
A Cell

AbstractThe extent to which cancer-associated alterations in extracellular matrix stiffness influences the crosstalk between cancer cells and mesenchymal stem cells (MSCs) remains unclear. By analyzing multiple singlecell RNA sequencing datasets, we establish the existence of a cell sub-population co-expressing MSC and cancer associated fibroblast (CAF) markers in highly aggressive triple-negative breast cancers in primary tumor, secondary sites, and in circulatory tumor cell clusters. Using hydrogels of varying stiffness corresponding to different stages of cancer progression, we show that on pre-metastatic stroma mimetic 2 kPa gels, MDA-MB-231 breast cancer cell secreted conditioned media drives efficient MSC chemotaxis and induces stable CAF differentiation in a TGFβ/contractility-dependent manner. In addition to enhancing cancer cell proliferation, 2 kPa CAFs maximally boost local invasion and confer resistance to flow-induced shear stresses. Together, our results suggest that homing of MSCs at the pre-metastatic stage and their differentiation into CAFs actively drives breast cancer invasion and metastasis.

scholarly journals Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component

2015 ◽  
Vol 67 (4) ◽  
pp. 1285-1295 ◽  
Author(s):  
Nina Petrovic ◽  
Snezana Jovanovic-Cupic ◽  
Goran Brajuskovic ◽  
Silvana Lukic ◽  
Jelena Roganovic ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Micro Rna ◽  
Prognostic Parameters ◽  
Breast Carcinomas ◽  
Expression Levels ◽  
Non Invasive ◽  
Invasive Component ◽  
Her 2 ◽  
Ductal Carcinomas ◽  
The Difference

Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (?=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.

scholarly journals High-content siRNA 3D co-cultures to identify myoepithelial cell-derived breast cancer suppressor proteins

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Hendrika M. Duivenvoorden ◽  
Natasha K. Brockwell ◽  
Cameron J. Nowell ◽  
Kaylene J. Simpson ◽  
Belinda S. Parker
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Early Breast Cancer ◽  
Myoepithelial Cell ◽  
Myoepithelial Cells ◽  
Cancer Invasion ◽  
Breast Cancers ◽  
Analysis Pipeline ◽  
Breast Cancer Invasion ◽  
Insight Into

AbstractUnderstanding how cancer cells interact with the surrounding microenvironment early in breast cancer development can provide insight into the initiation and progression of invasive breast cancers. The myoepithelial cell layer surrounding breast ducts acts as a physical barrier in early breast cancer, preventing cancer cells from invading the surrounding stroma. Changes to the expression profile and properties of myoepithelial cells have been implicated in progression to invasive carcinoma. Identifying the molecular drivers of myoepithelial cell-mediated tumour suppression may offer new approaches to predict and block the earliest stages of cancer invasion. We employed a high-content approach to knock down 87 different genes using siRNA in an immortalised myoepithelial cell line, prior to co-culture with invasive breast cancer cells in 3D. Combined with high-content imaging and a customised analysis pipeline, this system was used to identify myoepithelial proteins that are necessary to control cancer cell invasion. This dataset has identified prospective myoepithelial suppressors of early breast cancer invasion which may be used by researchers to investigate their clinical validity and utility.

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