GATA family of transcription factors of vertebrates: phylogenetics and chromosomal synteny

2007 ◽  
Author(s):  
Chunjiang He ◽  
Hanhua Cheng ◽  
Rongjia Zhou
Keyword(s):  
Transcription Factors ◽  
Gata Family ◽  
Chromosomal Synteny

GATA family of transcription factors of vertebrates: phylogenetics and chromosomal synteny

2007 ◽  
Vol 32 (S3) ◽  
pp. 1273-1280 ◽  
Author(s):  
Chunjiang He ◽  
Hanhua Cheng ◽  
Rongjia Zhou
Keyword(s):  
Transcription Factors ◽  
Gata Family ◽  
Chromosomal Synteny

scholarly journals Development and Carcinogenesis: Roles of GATA Factors in the Sympathoadrenal and Urogenital Systems

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 299
Author(s):  
Takashi Moriguchi
Keyword(s):  
Transcription Factors ◽  
Developmental Process ◽  
Current Knowledge ◽  
Gynecologic Cancers ◽  
Inherited Diseases ◽  
Gata Factors ◽  
Transcriptional Regulatory ◽  
Gata Family

The GATA family of transcription factors consists of six proteins (GATA1-6) that control a variety of physiological and pathological processes. In particular, GATA2 and GATA3 are coexpressed in a number of tissues, including in the urogenital and sympathoadrenal systems, in which both factors participate in the developmental process and tissue maintenance. Furthermore, accumulating studies have demonstrated that GATA2 and GATA3 are involved in distinct types of inherited diseases as well as carcinogenesis in diverse tissues. This review summarizes our current knowledge of how GATA2 and GATA3 participate in the transcriptional regulatory circuitry during the development of the sympathoadrenal and urogenital systems, and how their dysregulation results in the carcinogenesis of neuroblastoma, renal urothelial, and gynecologic cancers.

scholarly journals DNA-binding specificity of GATA family transcription factors.

1993 ◽  
Vol 13 (7) ◽  
pp. 3999-4010 ◽  
Author(s):  
M Merika ◽  
S H Orkin
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Mammalian Cells ◽  
Chimeric Protein ◽  
Binding Specificity ◽  
Mobility Shift ◽  
Binding Domains ◽  
Dna Binding Specificity ◽  
Mobility Shift Assay ◽  
Gata Family

GATA-binding proteins constitute a family of transcription factors that recognize a target site conforming to the consensus WGATAR (W = A or T and R = A or G). Here we have used the method of polymerase chain reaction-mediated random site selection to assess in an unbiased manner the DNA-binding specificity of GATA proteins. Contrary to our expectations, we show that GATA proteins bind a variety of motifs that deviate from the previously assigned consensus. Many of the nonconsensus sequences bind protein with high affinity, equivalent to that of conventional GATA motifs. By using the selected sequences as probes in the electrophoretic mobility shift assay, we demonstrate overlapping, but distinct, sequence preferences for GATA family members, specified by their respective DNA-binding domains. Furthermore, we provide additional evidence for interaction of amino and carboxy fingers of GATA-1 in defining its binding site. By performing cotransfection experiments, we also show that transactivation parallels DNA binding. A chimeric protein containing the finger domain of areA and the activation domains of GATA-1 is capable of activating transcription in mammalian cells through GATA motifs. Our findings suggest a mechanism by which GATA proteins might selectively regulate gene expression in cells in which they are coexpressed.

scholarly journals Regulation of Sphingosine-1-phosphate Lyase Gene Expression by Members of the GATA Family of Transcription Factors

2005 ◽  
Vol 280 (18) ◽  
pp. 18403-18410 ◽  
Author(s):  
Babak Oskouian ◽  
Jane Mendel ◽  
Ellyn Shocron ◽  
Michael A. Lee ◽  
Henrik Fyrst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Sphingosine 1 Phosphate ◽  
Gata Family

scholarly journals Expression of GATA family of transcription factors in T-cells, monocytes and bronchial biopsies

2001 ◽  
Vol 18 (3) ◽  
pp. 466-473 ◽  
Author(s):  
G. Caramori ◽  
S. Lim ◽  
K. Ito ◽  
K. Tomita ◽  
T. Oates ◽  
...  
Keyword(s):  
T Cells ◽  
Transcription Factors ◽  
Bronchial Biopsies ◽  
Gata Family

scholarly journals Mutational Analysis of AREA, a Transcriptional Activator Mediating Nitrogen Metabolite Repression in Aspergillus nidulans and a Member of the “Streetwise” GATA Family of Transcription Factors

1998 ◽  
Vol 62 (3) ◽  
pp. 586-596 ◽  
Author(s):  
Richard A. Wilson ◽  
Herbert N. Arst
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Dna Binding ◽  
Aspergillus Nidulans ◽  
Transcriptional Activator ◽  
Structural Genes ◽  
Gata Factor ◽  
Mutational Change ◽  
Nitrogen Metabolite Repression ◽  
Gata Family

SUMMARY The transcriptional activator AREA is a member of the GATA family of transcription factors and mediates nitrogen metabolite repression in the fungus Aspergillus nidulans. The nutritional versatility of A. nidulans and its amenability to classical and reverse genetic manipulations make the AREA DNA binding domain (DBD) a useful model for analyzing GATA family DBDs, particularly as structures of two AREA-DNA complexes have been determined. The 109 extant mutant forms of the AREA DBD surveyed here constitute one of the highest totals of eukaryotic transcription factor DBD mutants, are discussed in light of the roles of individual residues, and are compared to corresponding mutant sequence changes in other fungal GATA factor DBDs. Other topics include delineation of the DBD using both homology and mutational truncation, use of frameshift reversion to detect regions of tolerance to mutational change, the finding that duplication of the DBD can apparently enhance AREA function, and use of the AREA system to analyze a vertebrate GATA factor DBD. Some major points to emerge from work on the AREA DBD are (i) tolerance to sequence change (with retention of function) is surprisingly great, (ii) mutational changes in a transcription factor can have widely differing, even opposing, effects on expression of different structural genes so that monitoring expression of one or even several structural genes can be insufficient and possibly misleading, and (iii) a mutational change altering local hydrophobic packing and DNA binding target specificity can markedly influence the behavior of mutational changes elsewhere in the DBD.

scholarly journals Members of the GATA Family of Transcription Factors Bind to the U3 Region of Cas-Br-E and Graffi Retroviruses and Transactivate Their Expression

1998 ◽  
Vol 72 (7) ◽  
pp. 5579-5588 ◽  
Author(s):  
Corinne Barat ◽  
Eric Rassart
Keyword(s):  
Transcription Factors ◽  
Binding Sites ◽  
Mobility Shift ◽  
Dnase I Footprinting ◽  
Nuclear Extracts ◽  
Gel Mobility Shift ◽  
Leukemia Viruses ◽  
Immature Cells ◽  
Granulocytic Leukemia ◽  
Gata Family

ABSTRACT Cas-Br-E and Graffi are two murine viruses that induce myeloid leukemia in mice: while Cas-Br-E induces mostly non-T, non-B leukemia composed of very immature cells, Graffi causes exclusively a granulocytic leukemia (E. Rassart, J. Houde, C. Denicourt, M. Ru, C. Barat, E. Edouard, L. Poliquin, and D. Bergeron, Curr. Top. Microbiol. Immunol. 211:201–210, 1995). In an attempt to understand the basis of the myeloid specificity of these two retroviruses, we used DNase I footprinting analysis and gel mobility shift assays to identify a number of protein binding sites within the Cas-Br-E and Graffi U3 regions. Two protected regions include potential GATA binding sites. Methylation interference analysis with different hematopoietic nuclear extracts showed the importance of the G residues in these GATA sites, and supershift assays clearly identified the binding factors as GATA-1, GATA-2, and GATA-3. Transient assays with long terminal repeat (LTR)-chloramphenicol acetyltransferase constructs showed that these three GATA family members are indeed able to transactivate Cas-Br-E and Graffi LTRs. Thus, the availability and relative abundance of the various members of the GATA family of transcription factors in a given cell type could influence the transcriptional tissue specificity of murine leukemia viruses and hence their disease specificity.

scholarly journals The Level of DAL80 Expression Down-Regulates GATA Factor-Mediated Transcription inSaccharomyces cerevisiae

2000 ◽  
Vol 182 (23) ◽  
pp. 6584-6591 ◽  
Author(s):  
Thomas S. Cunningham ◽  
Rajendra Rai ◽  
Terrance G. Cooper
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Binding Sites ◽  
Nitrogen Availability ◽  
Transport Proteins ◽  
Catabolic Gene ◽  
Nitrogen Catabolite Repression ◽  
Gata Factor ◽  
Regulatory Circuit ◽  
Gata Family

ABSTRACT Nitrogen-catabolic gene expression in Saccharomyces cerevisiae is regulated by the action of four GATA family transcription factors: Gln3p and Gat1p/Nil1p are transcriptional activators, and Dal80 and Deh1p/Gzf3p are repressors. In addition to the GATA sequences situated upstream of all nitrogen catabolite repression-sensitive genes that encode enzyme and transport proteins, the promoters of the GAT1, DAL80, andDEH1 genes all contain multiple GATA sequences as well. These GATA sequences are the binding sites of the GATA family transcription factors and are hypothesized to mediate their autogenous and cross regulation. Here we show, using DAL80 fused to the carbon-regulated GAL1,10 or copper-regulated CUP1 promoter, that GAT1expression is inversely regulated by the level of DAL80expression, i.e., as DAL80 expression increases,GAT1 expression decreases. The amount of DAL80expression also dictates the level at which DAL3, a gene activated almost exclusively by Gln3p, is transcribed. Gat1p was found to partially substitute for Gln3p in transcription. These data support the contention that regulation of GATA-factor gene expression is tightly and dynamically coupled. Finally, we suggest that the complicated regulatory circuit in which the GATA family transcription factors participate is probably most beneficial as cells make the transition from excess to limited nitrogen availability.

scholarly journals The GATA Family of Transcription Factors in Arabidopsis and Rice

2004 ◽  
Vol 134 (4) ◽  
pp. 1718-1732 ◽  
Author(s):  
José C. Reyes ◽  
M. Isabel Muro-Pastor ◽  
Francisco J. Florencio
Keyword(s):  
Transcription Factors ◽  
Gata Family
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