Characterization and differential expression of sucrose and starch metabolism genes in contrasting chickpea (Cicer arietinum L.) genotypes under low temperature

2021 ◽  
Vol 100 (2) ◽  
Author(s):  
KAMAL DEV SHARMA ◽  
GAURAV PATIL ◽  
ASHA KIRAN
Keyword(s):  
Low Temperature ◽  
Differential Expression ◽  
Cicer Arietinum ◽  
Starch Metabolism ◽  
Cicer Arietinum L

DIFERENCIACIÓN DE ACCESIONES DE GARBANZO MEDIANTE CARACTERES MORFOLÓGICOS Y MOLECULARES

2018 ◽  
Vol 41 (4) ◽  
pp. 469-476
Author(s):  
Ernestina Valadez-Moctezuma ◽  
Anselmo de J. Cabrera-Hidalgo
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L

El garbanzo (Cicer arietinum L.) es una de las principales leguminosas de grano cultivadas en el mundo. México es de los diez principales productores a nivel mundial con una producción total de 171 mil toneladas de grano. Este cultivo presenta alto nivel de autogamia y de monotonía genética, lo que dificulta la diferenciación de genotipos élite. En este estudio preliminar se evaluó la variabilidad de 57 genotipos de diferentes áreas geográficas mediante caracteres morfológicos y marcadores moleculares tipo RAPD e ISSR. El análisis de correspondencia múltiple mostró que los mayores valores discriminantes fueron el color de la flor azul y semillas grandes y de forma redondeada, pero el agrupamiento respectivo no diferenció a las accesiones, incluyendo las especies silvestres; sin embargo, el análisis UPGMA logró una mejor separación. Los marcadores RAPD aun cuando generaron perfiles de ADN, no fueron informativos, mientras que los ISSR diferenciaron a las 57 accesiones de C. arietinum utilizadas y a la especie silvestre C. reticulatum, lo que los hace buenos candidatos para caracterizar este cultivo. Este estudio sirvió como base para desarrollar otro sistema de marcadores moleculares más eficiente en esta especie.

scholarly journals The Candidate Genes Underlying a Stably Expressed QTL for Low Temperature Germinability in Rice (Oryza sativa L.)

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Tifeng Yang ◽  
Lian Zhou ◽  
Junliang Zhao ◽  
Jingfang Dong ◽  
Qing Liu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Differential Expression ◽  
Candidate Genes ◽  
Complex Traits ◽  
Molecular Breeding ◽  
Genome Wide Association Study ◽  
Oryza Sativa L ◽  
Sequence Comparisons ◽  
Cold Environments ◽  
Promising Source

Abstract Background Direct seeding is an efficient cultivation technique in rice. However, poor low temperature germinability (LTG) of modern rice cultivars limits its application. Identifying the genes associated with LTG and performing molecular breeding is the fundamental way to address this issue. However, few LTG QTLs have been fine mapped and cloned so far. Results In the present study, the LTG evaluation of 375 rice accessions selected from the Rice Diversity Panel 2 showed that there were large LTG variations within the population, and the LTG of Indica group was significantly higher than that of Japonica and Aus groups (p < 0.01). In total, eleven QTLs for LTG were identified through genome-wide association study (GWAS). Among them, qLTG_sRDP2–3/qLTG_JAP-3, qLTG_AUS-3 and qLTG_sRDP2–12 are first reported in the present study. The QTL on chromosome 10, qLTG_sRDP2–10a had the largest contribution to LTG variations in 375 rice accessions, and was further validated using single segment substitution line (SSSL). The presence of qLTG_sRDP2–10a could result in 59.8% increase in LTG under 15 °C low temperature. The expression analysis of the genes within qLTG_sRDP2–10a region indicated that LOC_Os10g22520 and LOC_Os10g22484 exhibited differential expression between the high and low LTG lines. Further sequence comparisons revealed that there were insertion and deletion sequence differences in the promoter and intron region of LOC_Os10g22520, and an about 6 kb variation at the 3′ end of LOC_Os10g22484 between the high and low LTG lines, suggesting that the sequence variations of the two genes could be the cause for their differential expression in high and low LTG lines. Conclusion Among the 11 QTLs identified in this study, qLTG_sRDP2–10a could also be detected in other three studies using different germplasm under different cold environments. Its large effect and stable expression make qLTG_sRDP2–10a particularly valuable in rice breeding. The two genes, LOC_Os10g22484 and LOC_Os10g22520, were considered as the candidate genes underlying qLTG_sRDP2–10a. Our results suggest that integrating GWAS and SSSL can facilitate identification of QTL for complex traits in rice. The identification of qLTG_sRDP2–10a and its candidate genes provide a promising source for gene cloning of LTG and molecular breeding for LTG in rice.

Enhancement in Plant Growth and Zinc Biofortification of Chickpea (Cicer arietinum L.) by Bacillus altitudinis

2021 ◽  
Author(s):  
Prity Kushwaha ◽  
Ruchi Srivastava ◽  
Kuppusamy Pandiyan ◽  
Arjun Singh ◽  
Hillol Chakdar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Cicer Arietinum ◽  
Bacillus Altitudinis ◽  
Cicer Arietinum L
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