Isolation and Characterization of Antidermatophytic Bioactive Molecules from Piper longum L. Leaves

Fermented foods are known for several health benefits, and they are generally used among the Asian people. Microorganisms involved in the fermentation process are most responsible for the final quality of the food. Traditional fermented (spontaneous fermentation) foods are a versatile source of bioactive molecules and bioactive microbes. Several reports are available regarding the isolation and characterization of potent strains from traditional fermented foods. A collection of information for easy literature analysis of bioactive microbes derived from Thai fermented food is not yet available. The current manuscript compiled information on bioactive (antimicrobial- and enzyme-producing probiotic) microbes isolated from naturally fermented Thai foods.

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Olive Mill Wastes: A Source of Bioactive Molecules for Plant Growth and Protection against Pathogens

Biology ◽

10.3390/biology9120450 ◽

2020 ◽

Vol 9 (12) ◽

pp. 450

Author(s):

Fabio Sciubba ◽

Laura Chronopoulou ◽

Daniele Pizzichini ◽

Vincenzo Lionetti ◽

Claudia Fontana ◽

...

Keyword(s):

Plant Growth ◽

Plant Pathogens ◽

Basic Research ◽

Bioactive Molecules ◽

Isolation And Characterization ◽

Long Time ◽

Olive Mill Wastes ◽

Positive Effect ◽

Olive Mill

Olive oil production generates high amounts of liquid and solid wastes. For a long time, such complex matrices were considered only as an environmental issue, due to their polluting properties. On the other hand, olive mill wastes (OMWs) exert a positive effect on plant growth when applied to soil due to the high content of organic matter and mineral nutrients. Moreover, OMWs also exhibit antimicrobial activity and protective properties against plant pathogens possibly due to the presence of bioactive molecules including phenols and polysaccharides. This review covers the recent advances made in the identification, isolation, and characterization of OMW-derived bioactive molecules able to influence important plant processes such as plant growth and defend against pathogens. Such studies are relevant from different points of view. First, basic research in plant biology may benefit from the isolation and characterization of new biomolecules to be potentially applied in crop growth and protection against diseases. Moreover, the valorization of waste materials is necessary for the development of a circular economy, which is foreseen to drive the future development of a more sustainable agriculture.

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Identification and Evaluation of New Potential Inhibitors of Human Neuraminidase 1 Extracted from Olyra latifolia L.: A Preliminary Study

Biomedicines ◽

10.3390/biomedicines9040411 ◽

2021 ◽

Vol 9 (4) ◽

pp. 411

Author(s):

Camille Albrecht ◽

Zachée Louis Evariste Akissi ◽

Philomène Akoua Yao-Kouassi ◽

Abdulmagid Alabdul Magid ◽

Pascal Maurice ◽

...

Keyword(s):

Bioactive Molecules ◽

Biological Functions ◽

Inhibitory Effects ◽

Sialidase Activity ◽

Isolation And Characterization ◽

Pharmacological Targets ◽

The Family ◽

Therapeutic Properties ◽

Potential Inhibitors

Sialidases, also called neuraminidases, are involved in several human pathologies such as neurodegenerative disorders, cancers, as well as infectious and cardiovascular diseases. Several studies have shown that neuraminidases, such as neuraminidase 1 (NEU-1), may be promising pharmacological targets. Therefore, the discovery of new selective inhibitors of NEU-1 are necessary to better understand the biological functions of this sialidase. In the present study, we describe the isolation and characterization of nine known compounds from Olyra latifolia L. leaves. This plant, known to have several therapeutic properties, belongs to the family of Poaceae and is found in the neotropics and in tropical Africa and Madagascar. Among the purified compounds, feddeiketone B, 2,3-dihydroxy-1-(4-hydroxy-3,5-diméthoxyphényl)-l-propanone, and syringylglycerol were shown to present structural analogy with DANA, and their effects on membrane NEU-1 sialidase activity were evaluated. Our results show that they possess inhibitory effects against NEU-1-mediated sialidase activity at the plasma membrane. In conclusion, we identified new natural bioactive molecules extracted from Olyra latifolia as inhibitors of human NEU-1 of strong interest to elucidate the biological functions of this sialidase and to target this protein involved in several pathophysiological contexts.

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Antiamoebic Activity of Marine Sponge Spongia officinalis var. ceylonensis Dendy

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v17i1.22312 ◽

2015 ◽

Vol 17 (1) ◽

pp. 38-42 ◽

Cited By ~ 1

Author(s):

Vijai Lakshmi ◽

Sheela Ghosal

Keyword(s):

Soluble Fraction ◽

Pharmaceutical Journal ◽

Climatic Conditions ◽

Bioactive Molecules ◽

Isolation And Characterization ◽

Antiamoebic Activity ◽

Spongia Officinalis

Human amoebiasis due to infection by Entamoeba histolytica is mainly associated with morbidity thus affecting the quality of life and pace of developmental activities of countries with warm climatic conditions. A consistently high global incidence of this disease has been reported. In an endeavor to identify novel molecules with potent antiamoebic activity and lower side-effect, the present study was designed to investigate antiamoebic activity in Spongia officinalis extracts/fractions followed by isolation and characterization of bioactive molecules. The methanol extract of the S. officinalis showed promising in vitro and in vivo antiamoebic activity. On further fractionation, the activity was localized in the n-butanol soluble fraction from which the major purified fraction was identified as a mixture of alkaloids (xestospongins and araguspongins) by LCMS studies of the n-butanol soluble fraction DOI: http://dx.doi.org/10.3329/bpj.v17i1.22312 Bangladesh Pharmaceutical Journal 17(1): 38-42, 2014

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Canine colostrum exosomes: characterization, anti-oxidative capacity and influence on canine mesenchymal stem cell

10.21203/rs.2.15189/v1 ◽

2019 ◽

Author(s):

Antonio J Villatoro ◽

María del Carmen Martín-Astorga ◽

Cristina Alcoholado ◽

José Becerra

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Oxidative Capacity ◽

Antioxidant Effect ◽

Bioactive Molecules ◽

Passive Immunity ◽

Isolation And Characterization ◽

First Time

Abstract Background: Colostrum is a specific secretion of the mammary gland fundamental for the survival of the puppy during the first weeks after birth. It contains important bioactive molecules involved in the passive immunity and the maturation of various organs, highlighting small vesicles named exosomes. Exosomes have not been described yet in canine colostrum milk, where its role is crucial in dam-newborn communication for the development of the neonate. Results: Exosomes were abundant in canine colostrum milk and appeared with a characteristic cup-shaped morphology and well-defined round vesicles. Their size distribution was between 37−140 nm and western blot analysis showed an expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosomes cargo. We also found that exosomes modified proliferation and secretory profile in canine mesenchymal stem cells derived from bone marrow and adipose tissue, differently according to their origin. Besides, the exosomes of canine colostrum demonstrated a potent antioxidant effect. Conclusions: We described for the first time the isolation and characterization of milk exosomes from canine colostrum. Our findings highlight the abundant presence of exosomes in the colostrum of the canine species and contribute to explain their important antioxidant capacity after parturition and their role in the modulation of cell development and tissue differentiation in the newborn, where mesenchymal stem cells seem to play a key role.

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Canine colostrum exosomes: characterization, influence on canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

10.21203/rs.2.15189/v2 ◽

2019 ◽

Author(s):

Antonio J Villatoro ◽

María del Carmen Martín-Astorga ◽

Cristina Alcoholado ◽

José Becerra

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Oxidative Capacity ◽

Antioxidant Effect ◽

Bioactive Molecules ◽

Passive Immunity ◽

Isolation And Characterization ◽

First Time

Abstract Background Colostrum is a specific secretion of the mammary gland fundamental for the survival of the puppy during the first weeks after birth. It contains important bioactive molecules involved in the passive immunity and the maturation of various organs, highlighting small vesicles named exosomes. Exosomes have not been described yet in canine colostrum milk, where its role is crucial in dam-newborn communication for the development of the neonate. Results Exosomes were abundant in canine colostrum milk and appeared with a characteristic cup-shaped morphology and well-defined round vesicles. Their size distribution was between 37−140 nm and western blot analysis showed an expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosomes cargo. We also found that exosomes modified proliferation and secretory profile in canine mesenchymal stem cells derived from bone marrow and adipose tissue, differently according to their origin. Besides, the exosomes of canine colostrum demonstrated a potent antioxidant effect on canine fibroblast. Conclusions We described for the first time the isolation and characterization of milk exosomes from canine colostrum. Our findings highlight the abundant presence of exosomes in the colostrum of the canine species and contribute to explain their important antioxidant capacity after parturition and their role in the modulation of cell development and tissue differentiation in the newborn, where mesenchymal stem cells seem to play a key role.

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