Genetic relatedness of Trichoderma isolates antagonistic against Fusarium oxysporum f.sp. dianthi inflicting carnation wilt

2008 ◽  
Vol 53 (2) ◽  
pp. 130-138 ◽  
Author(s):  
V. Shanmugam ◽  
Vivek Sharma ◽  
Ananthapadmanaban
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
Trichoderma Isolates

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

Characterisation of Australian isolates of Fusarium oxysporum f. sp. cubense by DNA fingerprinting analysis

2000 ◽  
Vol 51 (8) ◽  
pp. 945 ◽  
Author(s):  
K. S. Gerlach ◽  
S. Bentley ◽  
N. Y. Moore ◽  
K. G. Pegg ◽  
E. A. B. Aitken
Keyword(s):  
Fusarium Oxysporum ◽  
Dna Fingerprinting ◽  
Genetic Relatedness ◽  
Dna Amplification ◽  
Vegetative Compatibility ◽  
Dna Fingerprint ◽  
Vegetative Compatibility Groups ◽  
Fingerprinting Analysis ◽  
Race 1 ◽  
Race 2

Genetic variation among Australian isolates of the fungus Fusarium oxysporum f. sp. cubense (Foc), which causes Fusarium wilt in banana, was examined using DNA amplification fingerprinting (DAF). Ninety-four isolates which represented Races 1, 2, 3, and 4, and vegetative compatibility groups (VCGs) 0120, 0124, 0125, 0128, 0129, 01211, 01213/16, and 01220 were analysed. The genetic relatedness among isolates within each VCG, and between the 8 different VCGs of Foc present in Australia was determined. The DNA fingerprint patterns were VCG-specific, with each VCG representing a unique genotype. The genetic similarity among isolates within each VCG ranged from 97% to 100%. Among the different VCGs of Foc, 3 major clusters were distinguished which corresponded with race. All Race 1 and 2 isolates (VCGs 0124, 0125, 0128, and 01220) were closely related and clustered together, the Race 3 isolates from Heliconia clustered separately, and all Race 4 isolates (VCGs 0120, 0129, 01211, and 01213/16) clustered together. Fifteen isolates from Alstonville, NSW, were characterised because although they were classified as Race 2 based on their recovery from cooking banana cultivars, they belonged in VCG 0124, which had previously contained only Race 1 isolates. The occurrence of more than one race within a VCG means that vegetative compatibility grouping cannot be used to assign pathotype to pathogenic race as previously thought. It was possible to distinguish the Race 1 and Race 2 isolates within VCG 0124 using DNA fingerprinting, as each race produced a unique DNA fingerprint pattern. Among the Australian isolates, DNA fingerprinting analysis identified 9 different VCGs and genotypes of Foc.

scholarly journals Antagonistic Action of Trichoderma Isolates against Fusarium oxysporum f. sp. lycopersci

2017 ◽  
Vol 6 (2) ◽  
pp. 258-265
Author(s):  
Akash Tomar ◽  
Lakshman Prasad ◽  
Abhishek Mishra ◽  
Sushma Sagar
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Action ◽  
Trichoderma Isolates

scholarly journals Selection of Potential Antagonistic Bacillus and Trichoderma Isolates from Tomato Rhizospheric Soil Against Fusarium oxysporum f.sp. lycoperscisi

2011 ◽  
Vol 6 (10) ◽  
pp. 523-531 ◽  
Author(s):  
D. Praveen Kumar ◽  
R. Thenmozhi ◽  
P.D. Anupama ◽  
A. Nagasathya ◽  
N. Thajuddin ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Rhizospheric Soil ◽  
Trichoderma Isolates ◽  
Selection Of

scholarly journals Phylogeny and Optimization of Trichoderma harzianum for Chitinase Production: Evaluation of Their Antifungal Behaviour against the Prominent Soil Borne Phyto-Pathogens of Temperate India

2021 ◽  
Vol 9 (9) ◽  
pp. 1962
Author(s):  
Fayaz A. Mohiddin ◽  
Shahid A. Padder ◽  
Arif H. Bhat ◽  
Mohammad A. Ahanger ◽  
Asif B. Shikari ◽  
...  
Keyword(s):  
Disease Control ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Chitinase Activity ◽  
Morphological Identification ◽  
Formulation Development ◽  
Chitinase Production ◽  
Trichoderma Isolates ◽  
Wilt Incidence

Trichoderma is the most commonly used fungal biocontrol agent throughout the world. In the present study, various Trichoderma isolates were isolated from different vegetable fields. In the isolated microflora, the colony edges varied from wavy to smooth. The mycelial forms were predominantly floccose with hyaline color and conidiophores among all the strains were highly branched. Based on morphological attributes, all the isolates were identified as Trichoderma harzianum. The molecular identification using multilocus sequencing ITS, rpb2 and tef1α, genes further confirmed the morphological identification. The average chitinase activity varied from 1.13 units/mL to 3.38 units/mL among the various isolates, which increased linearly with temperature from 15 to 30 °C. There was an amplified production in the chitinase production in the presence of Mg+ and Ca2+ and Na+ metal ions, but the presence of certain ions was found to cause the down-regulated chitinase activity, i.e., Zn2+, Hg2+, Fe2+, Ag+ and K+. All the chitinase producing Trichoderma isolates inhibited the growth of tested pathogens viz., Dematophora necatrix, Fusarium solani, Fusarium oxysporum and Pythium aphanidermatum at 25% culture-free filtrate concentration under in vitro conditions. Also, under in vivo conditions, the lowest wilt incidence and highest disease control on Fusarium oxysporum was observed in isolate BT4 with mean wilt incidence and disease control of 21% and 48%, respectively. The Trichoderma harzianum identified in this study will be further used in formulation development for the management of diseases under field conditions.

scholarly journals Biological control of fusarium wilt of tomato by Trichoderma isolates

2016 ◽  
Vol 69 ◽  
pp. 57-63 ◽  
Author(s):  
H. Ghazalibiglar ◽  
D.R.W. Kandula ◽  
J.G. Hampton
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Tomato Plant ◽  
Culture Collection ◽  
Research Centre ◽  
Presence And Absence ◽  
Trichoderma Isolates

Th is study investigated the ability of ten Trichoderma isolates to control the fusarium wilt pathogen of tomato Fusarium oxysporum f sp lycopersici as well as the eff ect of these isolates on tomato plant growth in the presence and absence of the pathogen The isolates were obtained from the Lincoln BioProtection Research Centre Culture Collection and were inoculated into seed raising mix (05 w/w) in two glasshouse studies Two Trichoderma isolates significantly (P

Characterization of Trichoderma isolates and assessment of antagonistic potential against Fusarium oxysporum f. sp. cumini

2020 ◽  
Vol 22 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Kavita Yadav ◽  
T. Damodaran ◽  
Nidhi Kumari ◽  
Kakoli Dutt ◽  
Ram Gopal ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Potential ◽  
Trichoderma Isolates
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