scholarly journals Inhibitory Power Test of Two Trichoderma Isolates in In Vitro Way Againts Fusarium oxysporum The Cause of Red Chilli Stem Rot

2019 ◽  
Vol 1232 ◽  
pp. 012020
Author(s):  
A Wachid ◽  
Sutarman
2020 ◽  
Vol 18 (4) ◽  
pp. 143
Author(s):  
MESAK TOMBE ◽  
DARMAWAN PANGERAN ◽  
TRI SAPTARI HARYANI

<p>ABSTRAK<br />Busuk batang vanili (BBV) yang disebabkan oleh Fusarium<br />oxysporum f.sp. vanillae (Fov) merupakan patogen penting pada vanili dan<br />menyebabkan kerugian besar setiap tahunnya di Indonesia. Jumlah<br />kerugian diperkirakan sebesar 3.000 ton atau sekitar US$ 16 juta.<br />Penelitian dilaksanakan di laboratorium dan rumah kaca Kelti Hama dan<br />Penyakit, Balai Penelitian Tanaman Rempah dan Obat, Bogor pada bulan<br />Februari 2010 sampai Agustus 2010. Tujuan penelitian adalah untuk<br />mengetahui pengaruh formula dengan bahan aktif minyak cengkeh dan<br />serai wangi terhadap penyakit BBV dan dampaknya terhadap kehidupan<br />mikroorganisme tanah. Dalam pelaksanaannya, kegiatan penelitian dilaku-<br />kan dalam 3 tahap yaitu : (1) uji formula in vitro, (2) uji formula in vivo,<br />dan (3) uji dampak formula terhadap mikroorganisme tanah. Percobaan<br />tahap 1, 2, dan 3 masing-masing terdiri atas 10, 6, dan 6 perlakuan,<br />dan.masing-masing disusun menggunakan rancangan acak lengkap dengan<br />3 ulangan. Materi yang dikaji dalam penelitian ini adalah (1) formula<br />dengan bahan aktif minyak cengkeh (formula standar), (2) formula dengan<br />bahan aktif minyak cengkeh dan serai wangi (formula baru), dan (3)<br />fungisida sintetis berbahan aktif mankozeb sebagai pembanding. Hasil uji<br />in vitro menunjukkan bahwa fungisida nabati CS (minyak cengkeh + serai<br />wangi) pada ke 3 tingkatan konsentrasi (terutama konsentrasi 400 ppm)<br />memperlihatkan efektivitas yang tinggi dalam menghambat pertumbuhan<br />miselium dan produksi spora patogen Fov.F117 dibandingkan perlakuan<br />lain dan kontrol. Hasil uji in vivo menunjukkan hal yang sama bahwa<br />fungisida nabati CS memperlihatkan efektivitas yang tinggi dalam<br />menekan intensitas serangan patogen BBV, terutama pada dosis aplikasi 5<br />ml/l. Dampak perlakuan terhadap kehidupan mikroorganisme tanah (fungi<br />dan bakteri) memperlihatkan bahwa penggunaan fungisida sintetis<br />mankozeb menghambat kehidupan mikroorganisme tanah 90-100% jika<br />dibandingkan dengan kontrol. Populasi mikroorganisme tanah pada semua<br />perlakuan fungisida nabati lebih tinggi jika dibandingkan dengan fungisida<br />mankozeb. Populasi mikroorganisme pada aplikasi fungisida nabati pada<br />dosis 5 ml/l tidak berbeda nyata dengan kontrol. Penelitian ini<br />menunjukkan bahwa penggunaan fungisida nabati minyak cengkeh<br />terutama jika dikombinasikan dengan minyak serai wangi mempunyai<br />prospek untuk digunakan dalam pengendalian penyakit BBV dengan<br />interval aplikasi 3-4 minggu sekali.<br />Kata kunci : Vanilla planifolia, Fusarium oxysporum f.sp, vanillae, busuk<br />batang vanili, minyak cengkeh, minyak serai wangi</p><p>ABSTRACT<br />Vanilla stem rot (VSR) disease caused by Fusarium oxysporum f.sp.<br />vanillae (Fov) is an important disease on vanilla and causes severe loss<br />annually in Indonesia. The total annual loss in production due to the VSR<br />was estimated to be 3,000 ton or about US$ 16 million. This research was<br />carried out in the laboratory and green house of Pest and Disease of<br />Research Institute for Spices and Medicinal Crops, Bogor from February<br />2010 until August 2010. The objective of this research was to study the<br />effect of formula using active compounds of clove and citronella oils on<br />the VSR disease and its impact on the survival of soil microorganisms.<br />The research activities were conducted in three stages, namely (1) in vitro<br />formula test; (2) in vivo formula test, and (3) impact test of formula on the<br />soil microorganisms. Materials studied in this research were (1) formula<br />with active compound of clove oil (standard), (2) formula with active<br />compound of clove and citronella oils (new formula), and (3) synthetic<br />fungicide with mancozeb active ingredient as comparison. Every phase of<br />this 3 stage experiment was arranged using completely randomized design<br />with three replicates. First, second, and third phases of the experiment<br />consisted of 10, 6, and 6 treatments, respectively. In vitro test results<br />carried out in the laboratory indicated that botanical formula CS (clove and<br />citronella oils of the three concentration levels, especially on the 400 ppm)<br />showed high effectiveness on inhibiting mycelium growth and spore<br />production of Fov.F117 pathogen compared to other treatments and<br />control. In vivo test in the green house indicated the same result that<br />botanical fungicide CS (clove dan citronella oils) showed high<br />effectiveness in suppressing VSR disease infection intensity, especially on<br />5 ml/l dosage. Application of mancozeb synthetic fungicide inhibited 90 –<br />100% soil microorganism livelihood compared to control. However, the<br />soil microorganism population on all botanical fungicides was higher than<br />on mancozeb fungicide. The microorganism population on the botanical<br />fungicide of 5 ml/l dosage was not significantly different from the control<br />(natural soil with no treatment). This research indicates that aplication of<br />botanical fungicide (combination between clove and citronella oils) with<br />3 - 4 weeks interval is prospectous to control VSR disease.<br />Key words : Vanilla planifolia, Fusarium oxysporum, f.sp. vanillae,<br />vanilla stem rot, clove oil, citronella oil</p>


2021 ◽  
Vol 9 (9) ◽  
pp. 1962
Author(s):  
Fayaz A. Mohiddin ◽  
Shahid A. Padder ◽  
Arif H. Bhat ◽  
Mohammad A. Ahanger ◽  
Asif B. Shikari ◽  
...  

Trichoderma is the most commonly used fungal biocontrol agent throughout the world. In the present study, various Trichoderma isolates were isolated from different vegetable fields. In the isolated microflora, the colony edges varied from wavy to smooth. The mycelial forms were predominantly floccose with hyaline color and conidiophores among all the strains were highly branched. Based on morphological attributes, all the isolates were identified as Trichoderma harzianum. The molecular identification using multilocus sequencing ITS, rpb2 and tef1α, genes further confirmed the morphological identification. The average chitinase activity varied from 1.13 units/mL to 3.38 units/mL among the various isolates, which increased linearly with temperature from 15 to 30 °C. There was an amplified production in the chitinase production in the presence of Mg+ and Ca2+ and Na+ metal ions, but the presence of certain ions was found to cause the down-regulated chitinase activity, i.e., Zn2+, Hg2+, Fe2+, Ag+ and K+. All the chitinase producing Trichoderma isolates inhibited the growth of tested pathogens viz., Dematophora necatrix, Fusarium solani, Fusarium oxysporum and Pythium aphanidermatum at 25% culture-free filtrate concentration under in vitro conditions. Also, under in vivo conditions, the lowest wilt incidence and highest disease control on Fusarium oxysporum was observed in isolate BT4 with mean wilt incidence and disease control of 21% and 48%, respectively. The Trichoderma harzianum identified in this study will be further used in formulation development for the management of diseases under field conditions.


Author(s):  
Ni Luh Gede Ambaradewi

Sclerotium rolfsii Sacc. is one of the important pathogens in peanuts. The controls that have been undertaken by farmers generally use synthetic pesticides. However, the use of synthetic pesticides has various negative impacts on the environment and human health. Therefore a technology is needed to produce environmentally friendly pesticides and effectively control stem rot. This study aims to determine the effectiveness of the Jeringau rhizome extract against S. Rolfsii fungi. The results showed that the crude extract of the Jeringau rhizome was able to inhibit the growth of S. rolfsii fungi in vitro on PDA media. Minimum Inhibitory Concentration (MIC) crude extract of jeringau rhizome was 0.50%. The inhibitory power of the extract with a concentration of 0.10% to 0.50% increased from 48.17% to 100%. The treatment of extract concentration of 0.50% showed fungicidal properties against S. rolfsii.


2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.


BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (17) ◽  
Author(s):  
Elena Pérez-Nadales ◽  
Antonio Di Pietro

2016 ◽  
Vol 34 (8) ◽  
pp. 135-141
Author(s):  
S. Al-Maghribi ◽  
◽  
Y. Hammad ◽  
B. Rezk ◽  
◽  
...  
Keyword(s):  

2021 ◽  
Vol 7 (3) ◽  
pp. 194
Author(s):  
Carmen Gómez-Lama Cabanás ◽  
Antonio J. Fernández-González ◽  
Martina Cardoni ◽  
Antonio Valverde-Corredor ◽  
Javier López-Cepero ◽  
...  

This study aimed to disentangle the structure, composition, and co-occurrence relationships of the banana (cv. Dwarf Cavendish) root endophytome comparing two phenological plant stages: mother plants and suckers. Moreover, a collection of culturable root endophytes (>1000) was also generated from Canary Islands. In vitro antagonism assays against Fusarium oxysporum f.sp. cubense (Foc) races STR4 and TR4 enabled the identification and characterization of potential biocontrol agents (BCA). Eventually, three of them were selected and evaluated against Fusarium wilt of banana (FWB) together with the well-known BCA Pseudomonas simiae PICF7 under controlled conditions. Culturable and non-culturable (high-throughput sequencing) approaches provided concordant information and showed low microbial diversity within the banana root endosphere. Pseudomonas appeared as the dominant genus and seemed to play an important role in the banana root endophytic microbiome according to co-occurrence networks. Fungal communities were dominated by the genera Ophioceras, Cyphellophora, Plecosphaerella, and Fusarium. Overall, significant differences were found between mother plants and suckers, suggesting that the phenological stage determines the recruitment and organization of the endophytic microbiome. While selected native banana endophytes showed clear antagonism against Foc strains, their biocontrol performance against FWB did not improve the outcome observed for a non-indigenous reference BCA (strain PICF7).


Mycoses ◽  
2021 ◽  
Author(s):  
Hamid Badali ◽  
Connie Cañete‐Gibas ◽  
Hoja Patterson ◽  
Carmita Sanders ◽  
Barbara Mermella ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 683
Author(s):  
Yang Cao ◽  
Wei Ding ◽  
Chang Liu

Seedling blight, caused by the fungus Fusarium oxysporum, significantly lowers rice production globally. Earlier reports have opined that endophytic bacteria strains could be possible biocontrol agents, but the mechanistic actions involved are still unclear. Therefore, this study aimed to isolate the endophytic bacteria with high inhibitory activity and elucidate its possible mechanisms for inducing resistance by metabolomics. The results showed that mdj-36 had the strongest in vitro pathogen inhibition of F. oxysporum, while mdj-34 displayed the lowest inhibitory activity identified as Bacillus velezensis strains. Metabolomic analyses demonstrated that B. velezensis mdj-36 growth medium could produce higher organic acids, terpenes, and diterpene than B. velezensis mdj-34. Further investigation revealed that ‘secondary bile acid biosynthesis’ and ‘glycerophospholipid metabolism’ pathways played essential roles in defense response towards F. oxysporum. This study’s findings provide a credible theoretical basis for the possible use of the B. velezensis strain against rice seedling blight.


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